Characterization of Glaciecola sp. enzymes involved in the late steps of degradation of sulfated polysaccharide ulvan extracted from Ulva ohnoi

Mondal, Ratna; Ohnishi, Kouhei

doi:10.1016/j.bbrc.2019.12.081

Cited by 9 publications

(5 citation statements)

References 23 publications

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“…LOR, F. agariphila KMM 3901, and Glaciecola sp. (Foran et al 2017;Salinas and French 2017;Reisky et al 2019;Mondal and Ohnishi 2020). Comparison of the CAZymes and sulfatases in Vibrio FNV 38 to these organisms reveals that the overall ulvan saccharifying capability of this organism is comparatively limited.…”

Section: Discussionmentioning

confidence: 99%

“…These bacteria had no homology to the previously reported ulvan lyases, were aerobic and isolated using conventional media. Several other ulvan lyases from organisms such as Formosa agariphila, Glaciecola sp., Pseudoalteromonas sp., Catenovulum maritimum, and Thalassomonas sp., have been identified and characterised since then and classified into five different CAZy families-PL24, PL25, PL28, PL37 and PL40 (Konasani et al 2018;Qin et al 2018;Li et al 2020;Mondal and Ohnishi 2020;Xu et al 2021;Wang et al 2022;Tang et al 2023). Ulvan lyases are the first enzymes that act on ulvan thereby breaking down the polysaccharide to smaller oligomers and creating a unsaturated end in the process.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical characterisation of a PL24 ulvan lyase from seaweed-associated Vibrio sp. FNV38

Rodrigues,

Jouanneau,

Fernandez-Fuentes

et al. 2023

J Appl Phycol

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Ulvan is a green macroalgal cell wall polysaccharide that has tremendous potential for valorisation due to its unique composition of sulphated rhamnose, glucuronic acid, iduronic acid and xylose. Several potential applications such as production of biofuels, bioplastics and other value-added products necessitate the breakdown of the polysaccharide to oligomers or monomers. Research on ulvan saccharifying enzymes has been continually increasing over the last decade, with the increasing focus on valorisation of seaweed biomass for a biobased economy. Lyases are the first of several enzymes that are involved in saccharifying the polysaccharide and several ulvan lyases have been structurally and biochemically characterised to enable their effective use in the valorisation processes. This study investigates the whole genome of Vibrio sp. FNV38, an ulvan metabolising organism and biochemical characteristics of a PL24 ulvan lyase that it possesses. The genome of Vibrio sp. FNV38 has a diverse CAZy profile with several genes involved in the metabolism of ulvan, cellulose, agar, and alginate. The enzyme exhibits optimal activity at pH 8.5 in 100 mM Tris–HCl buffer and 30 °C. However, its thermal stability is poor with significant loss of activity after 2 h of incubation at temperatures above 25 °C. Breakdown product analysis reveals that the enzyme depolymerised the polysaccharide predominantly to disaccharides and tetrasaccharides.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biochemical characterisation of a PL24 ulvan lyase from seaweed-associated Vibrio sp. FNV38

Rodrigues,

Jouanneau,

Fernandez-Fuentes

et al. 2023

J Appl Phycol

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“…To date, three PULs responsible for ulvan depolymerization have been researched in Glaciecola sp., Alteromonas sp., and F. agariphila. − Ulvan can be transformed into monosaccharides via a cascade system involving ulvan lyases, sulfatases, and unsaturated glucuronyl hydrolase in marine bacteria. On the basis of previous reports, we speculate that ulvan has the following metabolic pathways in marine bacteria: ulvan lyases (PLs) of the families PL24, PL25, PL28, PL37, or PL40 participate in the initial cleavage between Rha3S and GlcA/IdoA and form unsaturated uronic acid residues at the end of oligosaccharides, which is the first step in the full depolymerization of ulvan.…”

Section: Metabolic Pathways Of Algal Polysaccharidesmentioning

confidence: 99%

“…Schematic diagram of the metabolic pathway of ulvan. ,,, Rha3S, rhamnose-3-sulfate; Xyl, xylose; Xyl2S, xylose-2-sulfate; GlcA, d -glucuronic acid; IdoA, l -iduronic acid; and Δ, unsaturated uronic acid.…”

Section: Metabolic Pathways Of Algal Polysaccharidesmentioning

confidence: 99%

Insights into Algal Polysaccharides: A Review of Their Structure, Depolymerases, and Metabolic Pathways

Liang

et al. 2022

J. Agric. Food Chem.

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In recent years, marine macroalgae with extensive biomass have attracted the attention of researchers worldwide. Furthermore, algal polysaccharides have been widely studied in the food, pharmaceutical, and cosmetic fields because of their various kinds of bioactivities. However, there are immense barriers to their application as a result of their high molecular size, poor solubility, hydrocolloid nature, and low physiological activities. Unique polysaccharides, such as laminarin, alginate, fucoidan, agar, carrageenan, porphyran, ulvan, and other complex structural polysaccharides, can be digested by marine bacteria with many carbohydrate-active enzymes (CAZymes) by breaking down the limitation of glycosidic bonds. However, structural elucidation of algal polysaccharides, metabolic pathways, and identification of potential polysaccharide hydrolases that participate in different metabolic pathways remain major obstacles restricting the efficient utilization of algal oligosaccharides. This review focuses on the structure, hydrolase families, metabolic pathways, and potential applications of seven macroalgae polysaccharides. These results will contribute to progressing our understanding of the structure of algal polysaccharides and their metabolic pathways and will be valuable for clearing the way for the compelling utilization of bioactive oligosaccharides.

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“…Several other ulvan lyases from organisms such as Formosa agariphila, Glaciecola sp., Pseudoalteromonas sp., Catenovulum maritimum ., and Thalassomonas sp., have been identi ed and characterised since then and classi ed into ve different CAZY families-PL24, PL25, PL28, PL37 andPL40 (Konasani et al, 2018;Li et al, 2020;Mondal & Ohnishi, 2020;Qin et al, 2018;Wang et al, 2022;Xu et al, 2021b). In this study, we have explored the genome of Vibrio sp.…”

Section: Introductionmentioning

confidence: 99%

Biochemical Characterisation of a PL24 Ulvan Lyase from Seaweed-Associated Vibrio sp. FNV38

Rodrigues¹,

Jouanneau

Fernández-Fuentes³

et al. 2023

Preprint

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Ulvan is a green macroalgal cell wall polysaccharide that has tremendous potential for valorisation due to its unique composition of sulphated rhamnose, glucuronic acid, iduronic acid and xylose. Several potential applications necessitate the breakdown of the polysaccharide to oligomers or monomers. Research on ulvan saccharifying enzymes has been continually increasing over the last decade, with the increasing focus on valorisation of seaweed biomass for a biobased economy. Lyases are the first of several enzymes that are involved in saccharifying the polysaccharide and several ulvan lyases are being structurally and biochemically characterised to enable their effective use in the valorisation processes. This study investigates the whole genome of Vibrio sp. FNV38, an ulvan metabolising organism and biochemical characteristics of a PL24 ulvan lyase that it possesses. The genome of Vibrio sp. FNV38 has a diverse CAZy profile with several genes involved in the metabolism of ulvan, cellulose, agar, and alginate. The enzyme exhibits optimal activity at pH 8.5 in 100mM Tris-HCl buffer and 30oC. However, its thermal stability is poor with significant loss of activity after 2 hours of incubation at temperatures above 25oC. Breakdown product analysis reveals that the enzyme depolymerised the polysaccharide predominantly to disaccharides and tetrasaccharides. The enzyme is the first to be characterised from Vibrio sp. and has the potential to be genetically modified to improve its thermal stability for use in biorefinery processes.

show abstract

Characterization of Glaciecola sp. enzymes involved in the late steps of degradation of sulfated polysaccharide ulvan extracted from Ulva ohnoi

Cited by 9 publications

References 23 publications

Biochemical characterisation of a PL24 ulvan lyase from seaweed-associated Vibrio sp. FNV38

Biochemical characterisation of a PL24 ulvan lyase from seaweed-associated Vibrio sp. FNV38

Insights into Algal Polysaccharides: A Review of Their Structure, Depolymerases, and Metabolic Pathways

Biochemical Characterisation of a PL24 Ulvan Lyase from Seaweed-Associated Vibrio sp. FNV38

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