Characterization of glucose‐stimulated insulin release protocols in african green monkeys (Chlorocebus aethiops)

Abstract

Background Management of diabetes remains a major health and economic challenge, demanding test systems in which to develop new therapies. These studies assessed different methodologies for determining glucose tolerance in green monkeys. Methods Twenty‐eight African green monkeys between 4 and 24 years old underwent single or repeat intravenous glucose tolerance testing ( IVGTT ), oral glucose tolerance testing ( OGTT ), and/or graded glucose infusion testing. Results Geriatric monkeys exhibited glucose intolerance with impaired glucose‐stimulated insulin secretion following IVGTT . Repeat IVGTT and OGTT assessments were inconsistent. Monkeys with low glucose‐stimulated insulin secretion after graded glucose infusion exhibited elevated blood glucose levels. Conclusion IVGTT and graded glucose infusion protocols revealed differences in glucose tolerance among green monkeys at single time points, including age‐dependent differences suggestive of shifts in pancreatic beta‐cell functional capacity, but care should be applied to study design and the interpretation of data in the setting of longitudinal studies.

Ocular examination findings and selected ophthalmic diagnostic tests in African green monkeys (Chlorocebus aethiops sabaeus)

“…Mean IOP values ± SD were 14.1 ± 2.2 mmHg in JAGM and 18.0 ± 2.8 mmHg in AAGM (Table 2). The median (range) IOP for GAGM was 16.5 (27) mmHg. All values were found to be normally distributed except for IOP values for GAGM.…”

“…The subjects were grouped into three age categories informed by previous definitions, 27,28 and labeled as either juvenile (15-19 months), adult (5-9 years), or geriatric (19-30 years). The juvenile animals were housed in outdoor peer groups consisting of approximately 15 animals of similar ages and both sexes.…”