Characterization of glycerol kinase from baker's yeast: Response surface modeling of the enzymatic reaction

Aragon, Caio Casale; Ferreira‐Dias, Suzana; Gattás, Edwil Aparecida de Lucca; Peres, Maristela de Freitas Sanches

doi:10.1016/j.molcatb.2007.11.009

Cited by 7 publications

(3 citation statements)

References 46 publications

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“…d, i and ii) for interactions between lipid:surfactant ratio and stearin and between lipid+surfactant:water ratio and stearin ratio. This suggests that both the interactions did not produce a defined plateau or a maximum point, hence demonstrating that the interactions could not be optimized (Aragon et al, ). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Application of Response Surface Methodology for the Optimization of β‐Carotene‐Loaded Nanostructured Lipid Carrier from Mixtures of Palm Stearin and Palm Olein

Rohmah

Raharjo

Hidayat

et al. 2019

J Americ Oil Chem Soc

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The main purpose of this study was to optimize a β-carotene-loaded nanostructured lipid carrier (βC-NLC) using the lipid matrix of palm stearin and palm olein and Tween 80 as a surfactant. The NLC was prepared by using the high shear homogenization method. Box-Behnken Design (BBD) response surface methodology (RSM) was applied to optimize the process and formulation. A three-factor experimental model was used to optimize the combination of palm stearin ratio (A, %w/w), lipid:surfactant ratio (B, %w/w), and (lipid+surfactant):water ratio (C, %w/w). The formulations were evaluated for their responses on particle size (Y1), polydispersity index (Y2), zeta potential (Y3), and encapsulation efficiency (Y4). Subsequently, Fourier-transform infrared spectroscopy (FTIR), thermal (DT-TGA), x-ray diffraction (XRD), transmission electron microscopy (TEM), and in vitro release (Franz diffusion cell) analyses were utilized to observe the resulting optimum formulation. The optimum formulation was obtained at a combination of A (5.5:4.5), B (1:4.9), and C (24:76) %w/w. This resulted in βC-NLC having a particle size of 166 nm, polydispersity index of 0.35, zeta potential of −26.9 mV, and an encapsulation efficiency of 91.2%. No strong interaction between different NLC components was observed based on FTIR, DT-TGA, and XRD profiles. Round-shaped NLC particles were observed under TEM.Franz diffusion cell observation resulted in diffusion profile of β-carotene of 110.6 μg cm −2 with a flux of 1.06 (μg cm −2 hour −1 ). This indicates that palm stearin and palm olein can be prospectively developed as βC-NLC.Keywords β-Carotene Á Nanostructured lipid carriers Á Response surface methodology Á Box-Behnken design J Am Oil Chem Soc (2020) 97: 213-223.

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Section: Resultsmentioning

confidence: 99%

Application of Response Surface Methodology for the Optimization of β‐Carotene‐Loaded Nanostructured Lipid Carrier from Mixtures of Palm Stearin and Palm Olein

Rohmah

Raharjo

Hidayat

et al. 2019

J Americ Oil Chem Soc

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“…ε – molar extinction coefficient of the 4‐aminoantipyrine (6.65 × 10 3 m /cm as described by Aragon et al., ); and…”

Section: Methodsmentioning

confidence: 99%

Content of plasmatic glycerol and activity of hepatic glycerol kinase in broiler chickens fed diets containing different sources and concentrations of glycerine

Bernardino

Rodrigues

Naves

et al. 2013

Animal Physiology Nutrition

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The objective of this study was to evaluate the effect of three sources of glycerine (crude glycerine from soya bean oil - CGSO, mixed crude glycerine from frying oil and lard--MCG, and a semipurified glycerine from soya bean oil--SPGSO) in four concentrations in the diet (17.5, 35.0, 52.5 and 70.0 g of each type of glycerine per kg of feed) on the levels of plasmatic glycerol and the activity of the hepatic enzyme glycerol kinase in broilers of 22-35 days old (experiment I) and 33-42 days old (experiment II). The highest (p < 0.05) plasmatic glycerol level was detected in broilers fed diet containing CGSO. Independent of the source, increasing the concentration of glycerine led to a linear increase (p < 0.05) in the plasmatic glycerol concentration. In experiment I, all the diets containing glycerine resulted in increased concentrations of plasmatic glycerol, in relation to the control diet without glycerine. However, in experiment II, only the diet containing 17.5 g of CGSO per kg of feed and the diets formulated with any of the three types of glycerine in the inclusion concentrations of 35, 52.5 and 70 g/kg of feed resulted in higher plasmatic glycerol levels than those observed in the control broilers. The source of glycerine influenced the glycerol kinase activity only in experiment II, where the use of CGSO in the diet increased the enzyme activity. For both experiments, the glycerol kinase activity increased with the inclusion of glycerine in the diet. In conclusion, based on the absence of saturation of the glycerol kinase activity for the three glycerine sources and for both rearing periods evaluated, the broilers can metabolize the glycerol (at the level of the phosphorylation of the glycerol to glycerol-3-phosphate in the liver) present in the glycerine when the diet is supplemented with up to 70 g of glycerine per kg of feed.

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“…The RSM algorithm of action involves simultaneous tests of the variables that significantly affect the process, by using a minimal number of runs, therefore the RSM method is less expensive and less time-consuming as compared to the classical methods. [ 23 ] It has successfully been applied for development, improvement and also optimization of biotechnological processes. [ 24–27 ] The method has also been used to improve permeabilization processes.…”

Section: Introductionmentioning

confidence: 99%

Optimization of permeabilization process of yeast cells for catalase activity using response surface methodology

Trawczyńska

Wójcik

2015

Biotechnology & Biotechnological Equipment

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Biotransformation processes accompanied by whole yeast cells as biocatalyst are a promising area of food industry. Among the chemical sanitizers currently used in food technology, hydrogen peroxide is a very effective microbicidal and bleaching agent. In this paper, permeabilization has been applied to Saccharomyces cerevisiae yeast cells aiming at increased intracellular catalase activity for decomposed H2O2. Ethanol, which is non-toxic, biodegradable and easily available, has been used as permeabilization factor. Response surface methodology (RSM) has been applied in determining the influence of different parameters on permeabilization process. The aim of the study was to find such values of the process parameters that would yield maximum activity of catalase during decomposition of hydrogen peroxide. The optimum operating conditions for permeabilization process obtained by RSM were as follows: 53% (v/v) of ethanol concentration, temperature of 14.8 °C and treatment time of 40 min. After permeabilization, the activity of catalase increased ca. 40 times and its maximum value equalled to 4711 U/g.

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Characterization of glycerol kinase from baker's yeast: Response surface modeling of the enzymatic reaction

Cited by 7 publications

References 46 publications

Application of Response Surface Methodology for the Optimization of β‐Carotene‐Loaded Nanostructured Lipid Carrier from Mixtures of Palm Stearin and Palm Olein

Application of Response Surface Methodology for the Optimization of β‐Carotene‐Loaded Nanostructured Lipid Carrier from Mixtures of Palm Stearin and Palm Olein

Content of plasmatic glycerol and activity of hepatic glycerol kinase in broiler chickens fed diets containing different sources and concentrations of glycerine

Optimization of permeabilization process of yeast cells for catalase activity using response surface methodology

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