Characterization of Glycopeptide-Resistant Enterococci from a Swiss Hospital

Liassine, Nadia; Frei, Reno; Jan, Isabelle; Auckenthaler, Raymond

doi:10.1128/jcm.36.7.1853-1858.1998

Cited by 58 publications

(24 citation statements)

References 34 publications

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“…Enterococci are intrinsically resistant to several antibiotics. This natural resistance includes the cephalosporins, quinolones, aminosides, clindamycin and erythromycin (McDonald et al, 1997;Liassine et al, 1998). This is partly in accord with our results, which show that all isolates are resistant to cefoperazone, erythromycin and nalidix acid.…”

Section: Discussionsupporting

confidence: 90%

The Importance of Pets as Reservoirs of Resistant Enterococcus Strains, with Special Reference to Vancomycin

Rodrigues

Poeta

Martins

et al. 2002

Journal of Veterinary Medicine, Series B

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The purpose of the present study was to determine the prevalence of antibiotic resistance, with special attention to vancomycin, in 104 strains of Enterococcus, which is the sentinel bacterium isolated from dog and cat faeces samples. The phenotypic characterization of the isolates classified them as E. faecium (58%), E. avium (41%) and E. faecalis (1%). Sensitivity testing used the diffusion method according to the recommendations of CASFM (Comite de l'Antibiogramme de la Société Française de Microbiogie), to oxacillin, amoxycillin, amoxycilin + clavulanic acid, ampicillin, piperacillin, cefoperazone, imipenem, enrofloxacin, ciprofloxacin, ofloxacin, nalidixic acid, tetracycline, lincomycin, erythromicin and vancomycin. Minimum inhibitory concentration (MIC) determination by E test revealed a MIC to vancomycin of between 0.5 microg/ml and 3 microg/ml. All the strains were resistant to nalidixic acid, erythromycin, cefoperazone and oxacillin. We detected resistance to amoxycillin + clavulanic acid in 1.9% of isolates, to amoxycillin in 4.8%, to piperacillin in 13.5%, and to ampicillin in 21.2% of strains. A high prevalence of antibiotic resistance to lincomycin, tetracycline, enrofloxacin, ciprofloxacin and ofloxacin was found in 98.1%, 95.2%, 76.9%, 73.1% and 64.4% of strains, respectively. Resistance to vancomycin was not found, which indicates that there is no transmission risk of vancomycin-resistant enterococcal strains to man or transfer of their resistance genes to other bacteria belonging to the endogenous flora of humans.

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Section: Discussionsupporting

confidence: 90%

The Importance of Pets as Reservoirs of Resistant Enterococcus Strains, with Special Reference to Vancomycin

Rodrigues

Poeta

Martins

et al. 2002

Journal of Veterinary Medicine, Series B

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“…VanC confers only lowlevel resistance to vancomycin [24,25]. Compared to other phenotypes, the VanA is the most common in European countries [26,27]. Our results confirmed this fact: 100% of VREfm strains belong toVanA phenotype.…”

Section: Discussionsupporting

confidence: 81%

“…For identification, detection of the genes encoding D-alanine-D-alanine ligases specific for E. faecium (ddl E. faecium ) and for E. faecalis (ddl E. faecalis ) was performed. For detection of the vancomycin resistance genes, the attempt was made to identify the commonest ones, i.e., vanA, vanB, and vanC genotypes (vanC1 gene or vanC2/C3 gene) [12,13]. E. faecium BM4147 (vanA positive strain) was used as a positive control strain.…”

Section: Multiplex Polymerase Chain Reactionmentioning

confidence: 99%

The phenotypic and genotypic characterization of vancomycin-resistant enterococci in outpatients’ urine culture

et al. 2017

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Introduction/Objective In the era of emerging antibacterial resistance, the major burden of resistant strains is on hospitalized patients. Although community factors are also important in the spread of resistance, less attention has been paid to non-healthcare settings. The aim of the study is to determine the prevalence of vancomycin-resistant enterococci (VRE) in the outpatient's urine culture and to perform phenotypic and genotypic characterization of VRE strains. Methods During an 18-month period, a total of 5,164 Enterococcus spp. strains were isolated from urine and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antimicrobial susceptibility testing was performed by disk diffusion method and by gradient test for glycopeptideresistant strains. Genotypic characterization of VRE strains was done by multiplex polymerase chain reaction for the detection of the vancomycin resistance genes. Results Among the isolated enterococci, 5,060 (98%) were E. faecalis and 104 (2%) were E. faecium. E. faecalis strains were susceptible to all tested antibiotics except norfloxacin (33% of strains were resistant), while E. faecium showed high level of resistance to most of the tested agents (91.3% to ampicillin, 77% to norfloxacin, and 75% to nitrofurantoin), and 26% of strains were resistant to vancomycin and teicoplanin. VanA gene was detected in all vancomycin resistant E. faecium (VREfm) strains. Conclusion A high proportion of VREfm was noticed among outpatients in our country. All analyzed VREfm strains belonged to vanA genotype. Future surveillance studies of VRE are needed to follow up on this baseline study to monitor any possible changes in abundance and genotype of VRE in this population group.

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“…All the VanB-type isolates were found in one hospital ward and belonged to a common subgroup (Ib) together with five vanB-negative isolates (isolates 9, 10, 25 46 and 47). VanA-and VanB-type cassettes are known to be the most prevalent elements determining vancomycin resistance in E. faecalis (Liassine et al 1998). Our study confirms previous data on the relatively low incidence of VRE (Vancomycin-resistant enterococcus) in Poland in comparison with other countries, e.g.…”

Section: Titzementioning

confidence: 99%

Genotypic characterization of hospitalEnterococcus faecalisstrains using multiple-locus variable-number tandem-repeat analysis

Wałecka

Bania

Dworniczek

et al. 2009

Letters in Applied Microbiology

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Aims: The level of genetic diversity and relationships between the specific genotypes and the distribution of virulence determinants among Enterococcus faecalis strains isolated from patients hospitalized in different wards of two hospitals were investigated. Methods and Results: Fifty‐six clinical strains of E. faecalis, isolated from patients hospitalized in the period of 1999–2004 in several wards in Wrocław (Poland), were analysed by multiple‐locus variable‐number tandem‐repeat analysis (MLVA). Analysis of seven genomic loci identified 40 novel genotypes among the analysed E. faecalis strains, with two major genomic groups, designated I and II, distinguished at a cut‐off of 35%. With a similarity cut‐off of 85·7%, the genotypes could be combined into 12 clusters (C1–C12), containing at least two isolates. The remaining 18 MLVA types were represented by a single isolate. Conclusions: Based on the data obtained by MLVA, it was found that (i) many E. faecalis isolates recovered from patients from the wards whose location allowed the potential transmission of micro‐organisms, belonged to closely related MLVA types and (ii) possible relationships between specific E. faecalis genotype and the virulence factors lipase, haemolysin and esp gene can exist. Significance and Impact of the Study: Our study confirms that MLVA is a suitable method for the epidemiological study of E. faecalis and for the first time shows possible relationships between specific genotypes and such virulence determinants, i.e. lipase, haemolysin and esp gene.

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Characterization of Glycopeptide-Resistant Enterococci from a Swiss Hospital

Cited by 58 publications

References 34 publications

The Importance of Pets as Reservoirs of Resistant Enterococcus Strains, with Special Reference to Vancomycin

The Importance of Pets as Reservoirs of Resistant Enterococcus Strains, with Special Reference to Vancomycin

The phenotypic and genotypic characterization of vancomycin-resistant enterococci in outpatients’ urine culture

Genotypic characterization of hospitalEnterococcus faecalisstrains using multiple-locus variable-number tandem-repeat analysis

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