Characterization of Grass Pollen-Specific IgE, IgA, IgM Classes and IgG Subclasses in Allergic Patients

Desvaux, François-Xavier; Peltre, Gabriel; David, B.

doi:10.1159/000234961

Cited by 16 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There was high variability in the immunoblot pat terns obtained with plasma from both the IgG4+ and nor mal groups. The overlapping IgGl and IgG3 antibody binding patterns observed in normal plasma are consistent with those previously reported [18] with plasma from 10 Dactylis pollen-allergic patients. These similarities are ex plained in part by the juxtaposition of the Cy3 and Cy, genes on human chromosome 14 (30,31] (sec above).…”

Section: Discussionsupporting

confidence: 90%

“…IgG4 antibodies have been of particular in terest as a result of their possible role in blocking allergen from binding to IgE antibodies [4,8,9,16]. The parallel an tigen recognition observed with IgE and IgG4 antibodies specific for parasite [17] or, more recently, in our labora tory, with IgE and IgG4 antibodies specific for grass pollen [18] supports this hypothesis. However, the blocking activ ity for IgG4 antibodies has yet to be proven in vivo.…”

Section: Introductionsupporting

confidence: 56%

“…The binding patterns of the Dactylis pollen-specific IgG4 antibodies in the IgG4+ plasma were heterogeneous and distinct as compared to previous results from our group [18]. Interestingly, the total number and pi values of protein bands recognized by IgG4 antibodies from the IgG4+ plasma group was restricted to the same 20 pro teins detected by the IgE from these plasma samples, namely the allergen repertoire ( fig.…”

Section: R Esultscontrasting

confidence: 43%

See 2 more Smart Citations

Isotypic Analysis of Grass Pollen-Specific Immunoglobulins in Human Plasma

Batard

Basuyaux

Lambin

et al. 1993

Int Arch Allergy Immunol

View full text Add to dashboard Cite

The specificity and isotypic profile of humoral immune responses to Dactylis glomerata (Cocksfoot) pollen was studied by isoelectric focusing (IEF)-immunoprint analysis using 26 human plasma samples with high levels of Dactylis pollen-specific IgG4 (IgG4+ plasma) and 25 human plasma samples with low levels of specific IgG4 (normal plasma). Over 60 individual protein components in an aqueous pollen extract were separated by IEF and immunoprinted onto nitrocellulose (NC). Following plasma incubation, bound IgE, IgG1–4, IgA1, IgA2 and IgM antibodies were detected on separate immunoprints with isotype-specific antibodies. Binding patterns of IgG4 and the majority of IgG1 and IgA2 antibodies in the IgG4+ plasma group very closely paralleled the binding patterns produced by the IgE antibodies from the same plasma and are described as the ‘allergen repertoire’. In contrast, IgE, IgG4, IgG1 and IgA2 antibody reactivities to the ‘allergen repertoire’ were insignificant in the normal plasma group. These results suggest a qualitative, as well as a quantitative relationship between the immune responses which involve these 4 isotypes. Characteristic IgG2 and IgM antibody binding patterns, predominantly to non-allergenic antigens, were shared by the plasma from both groups, while IgG3 and IgA1 antibody binding patterns were highly variable from one plasma to another in both groups. One possible origin of the allergic diseases at the immunoglobulin heavy chain gene level is discussed.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionsupporting

confidence: 56%

Section: R Esultscontrasting

confidence: 43%

See 1 more Smart Citation

Isotypic Analysis of Grass Pollen-Specific Immunoglobulins in Human Plasma

Batard

Basuyaux

Lambin

et al. 1993

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…IgG4) as compared to nonexposed individuals [27]. The role of IgG4 for the allergic response is still questionable, as it was dis cussed by other investigators for several allergens [26,28,29], Furthermore, only allergic patients with the clinical symptoms of asthma and to a lesser degree in dividuals (bakers) without any symptoms possess spe cific IgE antibodies.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Allergenic Components of Rye and Wheat Flour (Secale,Triticumvulgaris) by Western Blot with Sera of Bakers: Their Effects on CD23 Expression

Franken

Stephan

Neuber

et al. 1991

Int Arch Allergy Immunol

View full text Add to dashboard Cite

The allergenic components of water-soluble rye flour extract were studied by immunoblotting. Sera from 100 bakers were analyzed for their IgG, IgG4 and IgE binding pattern. Two allergens with molecular weights of 35 and 14 kD were detected. Previously, the major allergens of wheat flour extract were identified. The wheat flour components at a MW of 15/17 kD and the rye flour component at a MW of 14 kD were purified and isolated. The modulation of the low affinity receptor for IgE (FcεRII/CD23) on monocytes by separated allergenic components was studied. Depending on the allergen concentration the CD23 expression on isolated cells increased after stimulation with the rye flour component (MW 14 kD). The combined addition of the rye flour component (14 kD) with IL-4 induced a significant CD23 expression as compared to IL-4 alone.

show abstract

“…Both CD23 and FcεRI are detectable on eosinophils in sites of allergic reactions (Tanaka et al 1995, Humbert et al 1996, including the airways (Chihara et al 1989, Humbert et al 1996. In mucosal sites, eosinophils will be present with antibodies of all three classes; and for inhaled allergens, IgG, IgE and IgA antibodies to relevant antigens are measurable in mucosal secretions (Platts-Mills 1979, Kitani et al 1985, Desvaux et al 1989. Interestingly, older studies of eosinophils had demonstrated that eosinophils were very effective in the uptake of antigen-antibody complexes (Sabesin 1963, Litt 1964a.…”

Section: Other Lymphocyte-eosinophil Interactive Mechanismsmentioning

confidence: 99%