Characterization of HCP-6, a <i>C. elegans</i> protein required to prevent chromosome twisting and merotelic attachment

Stear, Jeffrey H.; Roth, Mark B.

doi:10.1101/gad.989102

Cited by 85 publications

(117 citation statements)

References 40 publications

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“…Despite the differences in mitotic and meiotic chromosome architecture, condensin I occupies the same domains as AIR-2 in both processes (this study), and condensin II colocalizes with centromeric protein CENP-A in both processes (Chan et al, 2004;Hagstrom et al, 2002;Stear and Roth, 2002). Consistently, AIR-2 is required for condensin I recruitment in mitosis and for correct condensin I localization in meiosis, but not for condensin II targeting in either process.…”

Section: Meiotic Recruitment Of Condensin Complexessupporting

confidence: 66%

“…These data indicate that the timing of condensin loading onto chromosomes is conserved between worm and mammalian mitotic cells. From prometaphase to anaphase, the spatial patterns of condensins I and II were different, with condensin II in a centromere-like pattern (Hagstrom et al, 2002;Stear and Roth, 2002) and condensin I diffusely coating all chromosomes (Fig. 1B).…”

Section: Condensin Complexes In Mitosismentioning

confidence: 99%

“…In both systems, condensin II is enriched at the centromeres (Hagstrom et al, 2002;Ono et al, 2004;Stear and Roth, 2002). C. elegans AIR-2 (the Aurora B homolog) has also been reported to affect chromosomal association of MIX-1 and SMC-4, components of both condensins I and II (Hagstrom et al, 2002;Kaitna et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Different roles for Aurora B in condensin targeting during mitosis and meiosis

Collette

Petty

Golenberg

et al. 2011

Journal of Cell Science

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SummaryCondensin complexes are essential for mitotic and meiotic chromosome segregation. Caenorhabditis elegans, like other metazoans, has two distinct mitotic and meiotic condensin complexes (I and II), which occupy distinct chromosomal domains and perform nonredundant functions. Despite the differences in mitotic and meiotic chromosome behavior, we uncovered several conserved aspects of condensin targeting during these processes. During both mitosis and meiosis, condensin II loads onto chromosomes in early prophase, and condensin I loads at entry into prometaphase. During both mitosis and meiosis, the localization of condensin I, but not condensin II, closely parallels the localization of the chromosomal passenger kinase Aurora B (AIR-2 in C. elegans). Interestingly, condensin I and AIR-2 also colocalize on the spindle midzone during anaphase of mitosis, and between separating chromosomes during anaphase of meiosis. Consistently, AIR-2 affects the targeting of condensin I but not condensin II. However, the role AIR-2 plays in condensin I targeting during these processes is different. In mitosis, AIR-2 activity is required for chromosomal association of condensin I. By contrast, during meiosis, AIR-2 is not required for condensin I chromosomal association, but it provides cues for correct spatial targeting of the complex.

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Section: Meiotic Recruitment Of Condensin Complexessupporting

confidence: 66%

Section: Condensin Complexes In Mitosismentioning

confidence: 99%

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Different roles for Aurora B in condensin targeting during mitosis and meiosis

Collette

Petty

Golenberg

et al. 2011

Journal of Cell Science

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“…HCP-6 is the C. elegans homolog of CAP-D3, a non-SMC subunit of condensin II (24,28,29). In contrast to the SMC subunits SMC-4 and MIX-1 (9,24), the accumulation of HCP-6 on metaphase chromosomes requires CENP-A (24,29).…”

Section: Hcp-6 Depletion Results In a Condensation Defect Kineticallymentioning

confidence: 99%

Molecular analysis of mitotic chromosome condensation using a quantitative time-resolved fluorescence microscopy assay

Maddox

Portier

Desai

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

111

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Chromosomes condense during mitotic entry to facilitate their segregation. Condensation is typically assayed in fixed preparations, limiting analysis of contributing factors. Here, we describe a quantitative method to monitor condensation kinetics in living cells expressing GFP fused to a core histone. We demonstrate the utility of this method by using it to analyze the molecular requirements for the condensation of holocentric chromosomes during the first division of the Caenorhabditis elegans embryo. In control embryos, the fluorescence intensity distribution for nuclear GFP:histone changes during two distinct time intervals separated by a plateau phase. During the first interval, primary condensation converts diffuse chromatin into discrete linear chromosomes. After the plateau, secondary condensation compacts the curvilinear chromosomes to form shorter bar-shaped structures. We quantitatively compared the consequences on this characteristic profile of depleting the condensin complex, the mitosis-specific histone H3 kinase Aurora B, the centromeric histone CENP-A, and CENP-C, a conserved protein required for kinetochore assembly. Both condensin and CENP-A play critical but distinct roles in primary condensation. In contrast, depletion of CENP-C slows but does not prevent primary condensation. Finally, Aurora B inhibition has no effect on primary condensation, but slightly delays secondary condensation. These results provide insights into the process of condensation, help resolve apparent contradictions from prior studies, and indicate that CENP-A chromatin has an intrinsic role in the condensation of holocentric chromosomes that is independent of its requirement for kinetochore assembly. Aurora B ͉ CENP-A ͉ condensin ͉ kinetochore ͉ C. elegans

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“…[40][41][42] Additionally, budding yeast condensin complex mutants may also exhibit defects in kinetochore function. 34 Other A B C evidence points to a role for Top2p at kinetochores or centromere proximal heterochromatin.…”

Section: Discussionmentioning

confidence: 99%