Characterization of histamine receptors in isolated pig basilar artery by functional and radioligand binding studies

Miyamoto, Atsushi; Nishio, Akira

doi:10.1016/0024-3205(93)90570-s

Cited by 18 publications

(9 citation statements)

References 12 publications

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“…Its pA 2 value for bovine basilar arteries (7.61 _+ 0.11) was similar to that demonstrated with porcine basilar (7.77 -+ 0.06; Miyamoto and Nishio, 1993) and coronary arteries (7.80 -+ 0.07;Obi et al, 1991a). Its pA 2 value for equine basilar arteries (8.15 -+ 0.07) was virtually identical to that determined previously (8.14 -+ 0.06; Obi et al, 1991b).…”

Section: Discussionsupporting

confidence: 86%

“…negative logarithm of the molar concentration of antagomst that produces a twofold rightward shift oF a concentration-response curve; pECs0 , negative logarithm of ECs0; PGF2, prostaglandin Fz~; PGIy prostaglandin I z INTRODUCTION Species differences in the in vitro responsiveness of basilar arteries to histamine have been reported• Basilar arteries from dogs (Konishi et al, 1981;Abrol and Cook, 1984), guinea-pigs (Chang et al, 1988), rabbits (Ohashi et aL, 1985), pigs (Miyamoto and Nishio, 1993) and humans (Shibata et al, 1975) respond to histamine with contractions, whereas those from rats (Chang et al, 1988) and monkeys (Toda, 1990) respond with relaxations. Such differences in responsiveness may depend on different distributions of H 1-and H2-receptors on smooth-muscle or endothelial cells (Sercombe et al, 1986;Chang et al, 1988;Toda, 1990;Benedito et al, 1991), which are responsible for the release of nitric oxide (Palmer et aL, 1987) and prostaglandin 12 (PGI2) , Ea Kirn et al, 1988).…”

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confidence: 98%

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Vasomotor effects of histamine on bovine and equine basilar arteriesin vitro

Miyamoto

Nishio

1994

Vet Res Commun

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The vasomotor effects of histamine on isolated bovine and equine basilar arteries were examined. Histamine induced contractions in both these preparations. The maximal response to and pEC50 value for histamine of the equine artery were larger than those of bovine tissue. Similar results were obtained with endothelium-denuded basilar arteries. Diphenhydramine (H1-receptor antagonist) inhibited histamine-induced contractions of the basilar arteries from both species in a concentration-dependent manner and its pA2 values (with 95% confidence limits) were 7.61 (7.39-7.83) and 8.15 (8.01-8.29) for the bovine and equine preparations, respectively. Cimetidine (H2-receptor antagonist) slightly potentiated histamine-induced contractions of bovine, but not equine, basilar arteries. 2-Thiazolylethylamine (H1-receptor agonist) induced contractions in both preparations, whereas impromidine (H2-receptor agonist) induced weak relaxation of the bovine, but not the equine, tissue. These findings indicate that bovine basilar arterial smooth muscle cells possess H1- and H2-receptors. Stimulation of the former results in contraction, whereas stimulation of the latter results in weak relaxation. Equine basilar arterial smooth muscle cells possess H1-receptors, stimulation of which results in contraction.

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Section: Discussionsupporting

confidence: 86%

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confidence: 98%

Vasomotor effects of histamine on bovine and equine basilar arteriesin vitro

Miyamoto

Nishio

1994

Vet Res Commun

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“…Contraction of resting vascular tone in response to His has also been reported in guinea pigs [7], pigs [17], humans [22], horses and cattle [18]. The calculated pA 2 value for diphenhydramine was 6.62, which is lower than the values reported for bovine (7.61) and porcine (7.77) basilar arteries [17, 18]. A further study is needed to clarify the differences in pA 2 values among species.…”

Section: Discussionmentioning

confidence: 87%

Vasomotor Effects of Acetylcholine, Bradykinin, Noradrenaline, 5-Hydroxytryptamine, Histamine and Angiotensin II on the Mouse Basilar Artery

et al. 2014

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We investigated the responsiveness of the mouse basilar artery to acetylcholine (ACh), bradykinin (BK), noradrenaline (NA), 5-hydroxytryptamine (5-HT), histamine (His) and angiotensin (Ang) II in order to characterize the related receptor subtypes in vitro. ACh and BK induced endothelium-dependent relaxation of precontracted arteries with U-46619 (a thromboxane A2 analogue). Atropine (a non-selective muscarinic receptor antagonist) and Nω-nitro-L-arginine (a NO synthase inhibitor, L-NNA) shifted the concentration-response curve for ACh to the right, whereas pirenzepine, methoctramine and pFHHSiD (muscarinic M1, M2 and M3 antagonists, respectively) had no significant effect. L-NNA and HOE140 (a B2 antagonist) shifted the concentration-response curve for BK to the right, whereas des-Arg9-[Leu8]-BK (a B1 antagonist) and indomethacin (a cyclooxygenase inhibitor) had no significant effect. NA failed to produce any vasomotor action. His and Ang II induced concentration-dependent contraction. Diphenhydramine (a H1 antagonist) shifted the concentration-response curve for His to the right, whereas cimetidine (a H2 antagonist) had no significant effect. Losartan (an AT1 antagonist) shifted the concentration-response curve for Ang II to the right, whereas PD123319 (an AT2 antagonist) had no significant effect. These results suggest that the H1 and AT1 receptor subtypes might play an important role in arterial contraction, whereas muscarinic receptor subtypes apart from M1, M2 and M3, and B2 receptors on the endothelium, might modify these contractions to relaxations.

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“…The rinsed basilar arteries were minced with scissors and then homogenized in 8 volumes of 50 mM Tris-HCl buffer (pH 7.4) using a Polytron homogenizer at a setting of 8 for 8 periods of 15 sec with 45-sec intervals in an ice-bath. The membrane fraction of basilar arteries was prepared as described previously (12,13). Briefly, the homogenate was centrifuged at 500 x g for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Characterization of 5-Hydroxytryptamine Receptors on the Isolated Pig Basilar Artery by Functional and Radioligand Binding Studies.

1994

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ABSTRACT-5-Hydroxytryptamine(5-HT)-receptor subtypes on pig basilar arteries were investigated by measuring the contractile responses to 5-HT agonists, the effects of antagonists on the responses and by carry ing out a radioligand binding assay with [3H]5-HT. The rank order of contractile agonist potency (accord ing to the pEC50 values) was 5-carboxamidotryptamineThe contractile responses were not affected by endothelial denudation, and the 5-HT induced contractions were antagonized competitively by ketanserin. Methiothepin shifted the 5-HT concen tration-response curves to the right and downwards in a concentration-dependent manner. In the presence of ketanserin (10-6 M), however, methiothepin antagonized the 5-HT-induced contractions competitively.

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Characterization of histamine receptors in isolated pig basilar artery by functional and radioligand binding studies

Cited by 18 publications

References 12 publications

Vasomotor effects of histamine on bovine and equine basilar arteriesin vitro

Vasomotor effects of histamine on bovine and equine basilar arteriesin vitro

Vasomotor Effects of Acetylcholine, Bradykinin, Noradrenaline, 5-Hydroxytryptamine, Histamine and Angiotensin II on the Mouse Basilar Artery

Characterization of 5-Hydroxytryptamine Receptors on the Isolated Pig Basilar Artery by Functional and Radioligand Binding Studies.

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