Characterization of Human Myocardial Fibroblasts Immortalized by HPV16 E6–E7 Genes

Harms, Wolfgang; Rothämel, Thomas; Miller, Konstantin; Harste, Gabi; Grassmann, Marian; Heim, Albert

doi:10.1006/excr.2001.5274

Cited by 18 publications

(10 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To investigate the mechanism of CVB3 persistence in heart cells, CVB3 infection of primary human myocardial fibroblasts (HMF) or immortalized HMF was established (26)(27)(28). Unfortunately, cardiomyocytes but not fibroblasts represent the major target cells of CVB3 in the heart (35); thus, the suitability of persistently infected HMF cells for studying CVB3 infections in vitro is limited.…”

Section: Discussionmentioning

confidence: 99%

Virus-Host Coevolution in a Persistently Coxsackievirus B3-Infected Cardiomyocyte Cell Line

Pinkert

Klingel²,

Lindig

et al. 2011

J Virol

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Virus-Host Coevolution in a Persistently Coxsackievirus B3-Infected Cardiomyocyte Cell Line

Pinkert

Klingel²,

Lindig

et al. 2011

J Virol

View full text Add to dashboard Cite

show abstract

“…However, in the case of human cells, many of the cell lines treated with SV40-T are not truly immortal, but merely display a delay in the onset of senescence (Huschtscha and Holliday 1983). This is largely because the SV40 large T antigen inhibits p53 by binding and stabilizing it, unlike the HPV-16 E6 gene that while also binding to p53, promotes its degradation (Harms et al 2001). Moreover, E6 inhibits apoptosis and the transcriptional activation of p53 after DNA damage, making it a more suitable candidate for immortalization of somatic cells.…”

Section: Discussionmentioning

confidence: 98%

“…Previous research has shown that expression of HPV-16 E6 and E7 can efficiently immortalize different types of somatic human cells, such as fibroblasts (Shay et al 1993;Shiga et al 1997;Harms et al 2001), mammary epithelial cells (Shay et al 1993;Wazer et al 1995), foreskin keratinocytes (Halbert et al 1991), smooth muscle cells (Perez-Reyes et al 1992), chondrocytes (Chen et al 2006), and oral squamous cells (Kingsley et al 2006). The aims of this experiment were to investigate a method of transfection for Cherax quadricarinatus primary cultures and attempt to manipulate cell cycle regulation by transfecting cells with oncogenes, in an effort to induce an immortal cell line.…”

Section: Introductionmentioning

confidence: 99%

Attempts at immortalization of crustacean primary cell cultures using human cancer genes

Claydon

Owens

2008

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

Primary cell cultures from crustacea have been initiated since the 1960s, yet no permanent cell line is available. Primary cells have a limited proliferative capacity in culture due to cellular senescence, which is regulated by a group of dominant senescence genes. The aim of this research was to manipulate cell cycle regulation by transfecting Cherax quadricarinatus primary cells with oncogenes, in an effort to induce a permanent cell line. Human papillomaviruses (HPV) play a critical role in the formation of anogenital cancer. Research has demonstrated that the HPV-expressed E6 and E7 proteins function concomitantly to disrupt the p53 and retinoblastoma (Rb) tumor suppressor genes, regulators of the cell-cycle checkpoints at the first gap (G(1)) phase. HPV E6 and E7 genes were transfected into the C. quadricarinatus cells by lipofection. Successful transfection was demonstrated by the presence of oncogene messenger RNA by reverse transciptase polymerase chain reaction. At day 150, transfected cells still remain viable, although cell proliferation was stagnant. It may be that while transfection of the oncogenes was successful, no proliferation of the C. quadricarinatus cells was evident due to a lack of telomere maintenance.

show abstract

“…However, our findings do not support the hypothesis that apoptotic myxoma cells are related to HPV or EBV; there are even some similarities between HSV and HPV. [16][17][18][19][20][21][22][23] This investigation has several limitations. First, this retrospective study had a relatively small sample group.…”

Section: Discussionmentioning

confidence: 99%

“…19 In addition, the presence of the tumorigenic viruses, HSV and HPV, has been implicated to potentiate neoplastic conditions in mucin-enriched cornea and in conjunctiva. [20][21][22][23] HPV may play a major role in the development of rapidly progressive, multifocal transitional cell carcinoma in immunosuppressed patients. E6 and E7 genes of the oncogenic HPV-16 can be transfected into primary human myocardial fibroblasts, immortalizing them.…”

mentioning

confidence: 99%

Caspase-3-dependent apoptosis in cardiac myxoma: not associated with human papillomavirus or Epstein–Barr virus

et al. 2005

View full text Add to dashboard Cite

Cardiac myxoma is the most common tumor of the heart, has a variable clinical presentation and immunohistochemical profile. Viral infections, such as herpes simplex virus, human papillomavirus (HPV), and Epstein-Barr virus (EBV), may play an important role in the causes of cardiac myxoma. This investigation will demonstrate caspase-3-dependent apoptosis in cardiac myxoma without HPV or EBV infection. This study included 15 patients with cardiac myxoma, who were treated with surgical excision of the lesion. Data were collected on detailed clinical parameters. Terminal deoxynucleotidyl transferase nick-end labeling assay, electrophoresis, and caspase-3 immunohistochemical studies were performed to characterize apoptosis. Genechip containing 39 subtypes was used to elucidate HPV; and polymerase chain reaction to detect LMP-1 gene of EBV. The patient population comprised of eight (53%) women and seven (47%) men. The mean age of patient participants was 45 years, with an age range of 30-70 years. All patient cases were sporadic myxomas rather than familial myxomas. The patient presentations included dyspnea (53%), asymptomatic (27%), stroke (7%), chest pain (7%), and fever (7%). All lesions were located in the left atrium. The individual patient cases of myxoma did not differ in location or clinical event in terms of pathological scores, such as vascular proliferation, inflammation, cellularity, hyaline, calcification, or thrombosis. Cardiac myxoma is characterized by apoptosis through caspase-dependent pathway. HPV or EBV was not detected in any of the study patient samples. In conclusion, no viral genomes of HPV or EBV were detected in these 15 patients. This study demonstrates that caspase-3-dependent apoptosis in cardiac myxoma is not dependent on concurrence of previous HPV and/or EBV infection.

show abstract

Characterization of Human Myocardial Fibroblasts Immortalized by HPV16 E6–E7 Genes

Cited by 18 publications

References 53 publications

Virus-Host Coevolution in a Persistently Coxsackievirus B3-Infected Cardiomyocyte Cell Line

Virus-Host Coevolution in a Persistently Coxsackievirus B3-Infected Cardiomyocyte Cell Line

Attempts at immortalization of crustacean primary cell cultures using human cancer genes

Caspase-3-dependent apoptosis in cardiac myxoma: not associated with human papillomavirus or Epstein–Barr virus

Contact Info

Product

Resources

About