Characterization of human p53 antigens employing primate specific monoclonal antibodies

Thomas, R.; Kaplan, Leonard J.; Reich, Nancy C.; Lane, David P.; Levine, Arnold J.

doi:10.1016/0042-6822(83)90516-0

Cited by 70 publications

(47 citation statements)

References 28 publications

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“…Messenger RNA was isolated from SV80, C331 and Raji cells which all contain high levels of p53 protein and from HeLa cells which contain no detectable p53 (Benchimol et al, 1982;Thomas et al, 1983). Northern blot analysis ( Figure 5, panel A) shows that p53 mRNA was present in all the cell lines.…”

Section: Resultsmentioning

confidence: 99%

“…wts. Thomas et al, 1983). This may be due to post-translational modifications on the same primary amino acid sequence or to their having different but similar primary amino acid sequences.…”

Section: Discussionmentioning

confidence: 99%

“…This protein is also found in normal primate and murine fibroblasts (Benchimol et al, 1982;Chen et al, 1983; Thomas et al, 1983). However, the levels of p53 protein in these nontransformed cells are -100-fold less than in their corresponding transformed cells (Benchimol et al, 1982;Thomas et al, 1983). Several human transformed cell lines such as HeLa and EJ contain little or no detectable p53 (Benchimol et al, 1982;Thomas et at., 1983).…”

Section: Introductionmentioning

confidence: 99%

“…However, the levels of p53 protein in these nontransformed cells are -100-fold less than in their corresponding transformed cells (Benchimol et al, 1982;Thomas et al, 1983). Several human transformed cell lines such as HeLa and EJ contain little or no detectable p53 (Benchimol et al, 1982;Thomas et at., 1983). Thus, although p53 levels are generally elevated in transformed cells, this alone cannot be considered as a marker for transformation.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene.

Matlashewski¹,

Lamb²,

Pim³

et al. 1984

The EMBO Journal

258

136

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A cDNA clone for human p53 cellular tumor antigen has been isolated and characterized. This clone contains the complete 3′‐untranslated region and most of the open reading frame for the protein. Nucleotide sequence analysis revealed that p53 mRNA contains an Alu repeat in the 3′‐untranslated region. Hybridization selection experiments showed this clone was capable of selectively binding p53 mRNA. In vitro translation of SV80 mRNA resulted in the synthesis of two immunoreactive p53 polypeptide species. Northern blot analysis showed that human p53 mRNA was 2.8 kb in length and was present in cell lines containing high and low levels of p53 protein. There appears to be only a single p53 gene in human cells and Southern blot analysis demonstrated no major genomic rearrangements or amplification of the p53 gene in the transformed cell lines examined.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene.

Matlashewski¹,

Lamb²,

Pim³

et al. 1984

The EMBO Journal

258

136

View full text Add to dashboard Cite

show abstract

“…Normal p53 is found at low levels in virtually all mammalian cells (Rogel et al, 1985) and various studies have shown elevated levels of the mRNA and protein in a wide variety of tumours and tumour cell lines (Linzer & Levine, 1979;De Leo et al, 1979;Crawford et al, 1981;Benchimol et al, 1982;Rotter, 1983;Thomas et al, 1983) including breast cancer (Cattoretti et al, 1988;Thompson et al, 1990). The normal function of p53 is not known, but it is thought to be involved in the GO/GI to S transition in the cell cycle (Mercer et al, 1984;Ganon & Lane, 1987) where regulation of its activity may be through phosphorylation.…”

mentioning

confidence: 99%

Mutations in p53 do not account for heritable breast cancer: a study in five affected families

Prosser

Elder²,

Condie³

et al. 1991

Br J Cancer

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Expression of p53 protein in colorectal cancer and its relationship to short-term prognosis

Yamaguchi

Kurosaka

Fushida

et al. 1992

Cancer

127

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Background and Methods. The expression of p53 protein in 100 large bowel cancers was studied immuno‐histochemically by use of a monoclonal antibody (PAb1801). Results. Immunoreactivity was found in 61.0% of specimens from 100 patients with colorectal cancer. The pattern of p53 expression was mainly detected in the nuclei of the cancer cells. There was no significant correlation between the expression of p53 and the histologic grade, tumor size, serosal invasion, lymphatic invasion, venous invasion, lymph node metastasis, or liver metastasis. However, patients with p53‐positive tumors had a greater relative risk of death compared with those with p53‐negative tumors. The p53 negative‐tumors showed a recurrence rate of 5.9%; for the p53 positive‐tumors, a recurrence rate of 23.8% was recorded. The %year survival rate was 96.7% of 39 patients with p53‐negative carcinomas and 61.8% for the patients with p53‐positive tumors; there was a significant difference in the rate between the two groups of patients (P < 0.05). The growth fraction of p53‐positive tumors determined with a monoclonal antibody against DNA polymerase α (49.0%) was significantly higher than that of p53‐negative tumors (40.7%, P < 0.01). Conclusions. These results suggest that the immunoreactivity of p53 may be a biologic marker of prognostic significance.

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Characterization of human p53 antigens employing primate specific monoclonal antibodies

Cited by 70 publications

References 28 publications

Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene.

Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene.

Mutations in p53 do not account for heritable breast cancer: a study in five affected families

Expression of p53 protein in colorectal cancer and its relationship to short-term prognosis

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