Characterization of human pregnancy zone protein. Comparison with human alpha 2-macroglobulin.

Sand, Ole; Folkersen, J.; Westergaard, J.G.; Sottrup‐Jensen, Lars

doi:10.1016/s0021-9258(17)36319-6

“…The stratified means showed that there was dispersion due to the center effect but overall good agreement and consistency between independent European centers. In accordance with our results, the circulating level of pregnancy zone protein is known to be higher in women than men. − Furthermore, sex hormone-binding globulin has been reported to be significantly higher in normal nonpregnant women than in normal men , in addition to showing associations with obesity and diabetes. , …”

Section: Resultssupporting

confidence: 91%

Proteomic Biomarker Discovery in 1000 Human Plasma Samples with Mass Spectrometry

Cominetti

¹

,

Galindo

²

,

Corthésy

³

et al. 2015

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The overall impact of proteomics on clinical research and its translation has lagged behind expectations. One recognized caveat is the limited size (subject numbers) of (pre)clinical studies performed at the discovery stage, the findings of which fail to be replicated in larger verification/validation trials. Compromised study designs and insufficient statistical power are consequences of the to-date still limited capacity of mass spectrometry (MS)-based workflows to handle large numbers of samples in a realistic time frame, while delivering comprehensive proteome coverages. We developed a highly automated proteomic biomarker discovery workflow. Herein, we have applied this approach to analyze 1000 plasma samples from the multicentered human dietary intervention study "DiOGenes". Study design, sample randomization, tracking, and logistics were the foundations of our large-scale study. We checked the quality of the MS data and provided descriptive statistics. The data set was interrogated for proteins with most stable expression levels in that set of plasma samples. We evaluated standard clinical variables that typically impact forthcoming results and assessed body mass index-associated and gender-specific proteins at two time points. We demonstrate that analyzing a large number of human plasma samples for biomarker discovery with MS using isobaric tagging is feasible, providing robust and consistent biological results.

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“…However, other reports have been incapable of reproducing these results, which suggests that PZP is useless as a tumor marker [46]. Also, recent reports on the preparation and immunological reactivity of PZP allow us to presume that many previous studies may have been influenced by non-specific antibodies and/or patterns of contaminated proteins [47]. However, PZP protein overexpression in patients with MDS-RS and MDS-EB suggests that it may be useful as a tumor marker for plasmamedullary studies.…”

Section: Resultsmentioning

confidence: 99%

Frutalin Affinity Chromatography on Sepharose Gel as a Strategy for the Identification of Possible Tumor Markers in Myelodysplastic Syndromes

Romero¹,

Cavalcante²,

Monteiro-Moreira³

et al. 2022

Preprint

0

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Myelodysplastic syndromes (MDS) are diseases that occur when blood-producing cells in the bone marrow are damaged; such damage can affect one or more types of blood cells. Common types of MDS are refractory anemia with ring sideroblasts and refractory anemia with excess blasts (MDS-RS and MDS-EB, respectively). This work analyzed the proteomics of the medullary plasma of 10 patients with MDS-RS and MDS-EB compared to healthy control people. Overexpressed proteins that may be potential candidates for biological markers for the evaluation, study, and diagnosis of these diseases have been identified. These samples were subjected to immunodepleting, concentrated, and digested for further analysis by mass spectrometry. The ratios between selected groups and healthy people were calculated. Seven overexpressed proteins in both syndromes were identified as potential biomarker candidates: vitronectin (VTN), (2) fibrinogen (FGA), (3) pregnancy zone protein (PZP), (4) kininogen (KNG1), (5) immunoglobulin lambda chain (IGLL1), (6) complement factor C4b, and (7) hemopexin (HPX). A modified affinity chromatographic column with lectin frutalin (FTL) was used for non-depleted samples. Immunoglobulin M (IgM) was expressed in the samples from both syndromes. Surprisingly, IgM from patients with syndromes was over retained on the frutalin (FTL) column when compared with the control group. We further hypothesized that over retention of this protein by the FTL is due to the presence of α-galactosidic residues in the IgM of MDS-RS and MDS-EB patients. Differential recognition of proteins on non-depleted samples from the use of FTL appears to be a powerful tool for proteomic analysis.

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“…However, other reports have been incapable of reproducing these results, which suggests that PZP is useless as a tumor marker [46]. Also, recent reports on the preparation and immunological reactivity of PZP allow us to presume that many previous studies may have been influenced by non-specific antibodies and/or patterns of contaminated proteins [47]. However, PZP protein overexpression in patients with MDS-RS and MDS-EB suggests that it may be useful as a tumor marker for plasma-medullary studies.…”

Section: Resultsmentioning

confidence: 99%

Frutalin Affinity Chromatography on Sepharose Gel as a Strategy for the Identification of Possible Tumor Markers in Myelodysplastic Syndromes

Romero¹,

Romo.²,

Cavalcante³

et al. 2022

Preprint

0

View full text Add to dashboard Cite

Myelodysplastic syndromes (MDS) are diseases that occur when blood-producing cells in the bone marrow are damaged; such damage can affect one or more types of blood cells. Common types of MDS are refractory anemia with ring sideroblasts and refractory anemia with excess blasts (MDS-RS and MDS-EB, respectively). This work analyzed the proteomics of the medullary plasma of 10 patients with MDS-RS and MDS-EB compared to healthy control people. Overexpressed proteins that may be potential candidates for biological markers for the evaluation, study, and diagnosis of these diseases have been identified. These samples were subjected to immunodepleting, concentrated, and digested for further analysis by mass spectrometry. The ratios between selected groups and healthy people were calculated. Seven overexpressed proteins in both syndromes were identified as potential biomarker candidates: vitronectin (VTN), (2) fibrinogen (FGA), (3) pregnancy zone protein (PZP), (4) kininogen (KNG1), (5) immunoglobulin lambda chain (IGLL1), (6) complement factor C4b, and (7) hemopexin (HPX). A modified affinity chromatographic column with lectin frutalin (FTL) was used for non-depleted samples. Immunoglobulin M (IgM) was expressed in the samples from both syndromes. Surprisingly, IgM from patients with syndromes was over retained on the frutalin (FTL) column when compared with the control group. We further hypothesized that over retention of this protein by the FTL is due to the presence of α-galactosidic residues in the IgM of MDS-RS and MDS-EB patients. Differential recognition of proteins on non-depleted samples from the use of FTL appears to be a powerful tool for proteomic analysis.

show abstract

Characterization of human pregnancy zone protein. Comparison with human alpha 2-macroglobulin.

Cited by 114 publications

References 81 publications

Proteomic Biomarker Discovery in 1000 Human Plasma Samples with Mass Spectrometry

Proteomic Biomarker Discovery in 1000 Human Plasma Samples with Mass Spectrometry

Frutalin Affinity Chromatography on Sepharose Gel as a Strategy for the Identification of Possible Tumor Markers in Myelodysplastic Syndromes

Frutalin Affinity Chromatography on Sepharose Gel as a Strategy for the Identification of Possible Tumor Markers in Myelodysplastic Syndromes

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