Characterization of human rotavirus strains with G12 and P[9] detected in Japan

Abstract

Two G12 human rotavirus strains, CP727 and CP1030, were isolated from the respective diarrheic stools of an infant and an adult in Japan. VP7 gene sequences of strains CP727 and CP1030 showed high identity with that of the G12 prototype strain L26, and with those of G12 strains reported recently from Thailand, the United States, and India. VP4 gene sequences of strains CP727 and CP1030 showed the highest identity with those of P[9] rotaviruses. In Northern blot hybridization, strains CP727 and CP1030 were found to be closely related to strain AU-1 (G3P[9]); nine RNA segments hybridized to each other. Moreover, all segments each of the two Japanese G12 strains hybridized to those of the Thai G12 strain T152. These results suggest that Japanese G12 strains detected in this study are reassortants between a L26-like strain and a strain in the AU-1 genogroup. A similar reassortant was found in the Thai G12 strain T152.

Modification of rotavirus multiplex RT‐PCR for the detection of G12 strains based on characterization of emerging G12 rotavirus strains from South India

“…The sequence of this strain is distantly related to human G12 strains and does not segregate with any of the three lineages described. Northern blot hybridization analysis suggested that the G12 strains from Japan and Thailand (CP727, CP1030, and T152) were reassortants of a L26 like strain and a AU-I genogroup strain (G3) [Shinozaki et al, 2004]. Similar analysis for the ISO-1 and Se585 suggested that they may also be reassortants between L26 and strain US1205, a strain closely related to those in the DS-1 genogroup (G2) [Griffin et al, 2002].…”

“…The first human G12 reported in the Philippines was associated with a VP4 of P[4] in the 1980s. G12 rotaviruses found in the far East and South America, were all associated with P[9], and were closely related to each other within lineage II [Pongsuwanna et al, 2002;Shinozaki et al, 2004;Castello et al, 2006]. The G12 strains reported from the US, UK, Bangladesh, and India from 2002 cluster within lineage III, and the lack of diversity within the gene encoding the VP7 suggests both a common ancestor and a recent introduction [Griffin et al, 2002;Das et al, 2003].…”

Molecular Epidemiology of Rotavirus Diarrhea among Children in Saudi Arabia: First Detection of G9 and G12 Strains

“…In this regard, it may merit mention that there is significant population movement from South Asian countries to Saudi Arabia, which may facilitate the transmission of G12P [8] strains. Interestingly, G12 strains have now been detected with a variety of gene constellations, including long and short electropherotypes, and in association with three different P genotypes and two different genogroups (2,3,9,30,31,35,36). Such diversity observed in G12 strains strongly suggests the versatility of this strain to spread efficiently in the population through reassortment.…”

The detection and molecular characterization of human G12 genotypes in South Africa

“…However, between January 2003 and April 2005, serotype G12s accounted for 17.1% of all strains detected in eastern India . Serotype G12 strains have also been detected in South America [Pietruchinski et al, 2006], Europe [Banerjee et al, 2007;Banyai et al, 2007;Steyer et al, 2007b], Asia [Shinozaki et al, 2004;Uchida et al, 2006;Pun et al, 2007], and Australia [Kirkwood et al, 2006].…”