Characterization of human skin-derived mesenchymal stem cell proliferation rate in different growth conditions

Abstract

This study investigated conditions for optimal in vitro propagation of human skin-derived mesenchymal stem cells (S-MSC). Forty primary skin-derived precursor cell (SKP) cultures were established from both male and female donors (age 29-65 years) and eight of them were randomly selected for in-depth characterization. Effects of basic fibroblast growth factor (FGF-2), epidermal growth factor (EGF), leukemia inhibiting factor (LIF) and dibutyryl-cyclic adenosine monophosphate (db-cAMP) on S-MSC proliferation were investigated. Primary SKP cultures were >95% homogenous for CD90, CD73, and CD105 marker expression enabling to classify these cells as S-MSC. FGF-2 dose-dependent stimulation was observed in low serum medium only, whereas EGF neither stimulated S-MSC proliferation nor potentates the effect of FGF-2. Pronounced donor to donor differences among S-MSC cultures were observed in 3-day proliferation assay. This study demonstrates that homogenous S-MSC populations can be reproducibly isolated from individual donors of different age. Optimal cell culture conditions for in vitro propagation of S-MSC are B27 supplemented or low serum media with FGF-2 (4 ng/ml). EGF and LIF as well as db-cAMP are dispensable for S-MSC proliferation.

Treatment of Radiation Injury

“…53 In burns, resurfacing with a dermal component is required, and bFGF stimulates wound healing and enhances human skin-derived mesenchymal stem cells under serum-free conditions in a dosedependent manner. 54 In a mini-pig receiving 10-Gy external X-ray radiation to the skin and subcutaneous tissue with a tissue expander underneath the subcutaneous tissue, immediate use of bFGF after radiation successfully protected the tissue from subsequent damage by increasing epithelial proliferation, suppressing the induction of apoptotic cells, and enhancing angiogenic cells. 55 Radiation fibrosis is considered late radiation damage, and TGFb1 plays a pivotal role in the establishment of radiation fibrosis.…”

Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis

“…MSCs from the bone marrow (BM-MSCs) were originally described by Friedenstein et al as non-phagocytic and non-hematopoietic cells and adherent to plastic [4]. Over the last 15 years, there has been an explosion in the reports of MSCs isolated from a variety of other adult sources, including skin [5], adipose tissue [6], umbilical cord blood [7,8] and matrix, peripheral blood [9], tendons [10,11], amnion [12][13][14], and bone [15][16][17]. Their plasticity, immunosuppressive potential, immuno-modulatory properties [18,19], and trophic activity [20,21] make MSCs critical players in tissue homeostasis.…”

Enrichment and Characterization of Human Dermal Stem/Progenitor Cells by Intracellular Granularity

“…A cell population that can differentiate into adipogenic and osteogenic lineages has been isolated from hair follicles [56,57]. Therefore, we cannot rule out the possibility that SSC high -hDSPCs may be derived from hair follicles, which are present in the dermis of breast and abdomen as well as scalp.…”