Characterization of humoral and cellular immune responses elicited by reduced doses of Brucella abortus S19 (calfhood) vaccine in cattle calves of India

Shome, Rajeswari; Kilari, Sreenivasulu; Sahare, Amol; Kalleshamurthy, Triveni; Shome, Bibek Ranjan; Skariah, Somy; Hiremath, Jagadish; Misri, Jyoti; Rahman, Husna Abdul

doi:10.1016/j.rvsc.2023.03.017

Cited by 1 publication

(2 citation statements)

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“…Brucella abortus S 19 is a live attenuated vaccine, is used in many countries where thorough or 100% culling or slaughtering of infected animals are limited due to the lack of compensation procedures or schemes (Alton et al, 1988;Priyantha, 2011). The full dose of Brucella S 19 has been recommended by Shome et al, (2023) as 4x10 9 cfu/ml for calves. Therefore, the alternative is to continue vaccination to reduce incidences of abortions in endemic herds or regions.…”

Section: Discussionmentioning

confidence: 99%

“…Two different doses had been identified in Brucella S 19 vaccination in cattle and buffaloes, both high (high log of colony forming unit) and reduced doses (1-3 log reduction of colony forming unit) of Brucella abortus S 19 vaccination are practised globally; a high dose of Brucella abortus S 19 may cause prolonged persistent antibody responses, which interfere with serological screening in cattle and buffaloes in the field (Simpson et al, 2018;Ardiansyah et al, 2019). However, both doses or inoculums (100 times or 2 logs and 1000 times or 3 logs reduction of infective dose) had been shown a more or less similar in immune responses in cattle (Shome et al, 2023;Shome et al, 2020). Since it is a live vaccine, Brucella abortus S 19 multiplies in the natural host (Alton et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

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Detectable immunological response in Brucella abortus s 19 vaccinated herds of cattle in vilachchiya veterinary range, north central province, Sri Lanka

Herath,

Fernando,

Weerasooriya

et al. 2023

Sri Lanka Vet. J.

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Brucellosis a zoonotic disease caused by Gram-negative, intracellular, coccobacillus. Brucellosis, an economically important disease in livestock with zoonotic implications has been reported worldwide. It is transmitted via contaminated animals and their products. Clinical signs of animal brucellosis are abortions, reduced fertility, retention of fetal membranes, endometritis, and substantial decline in milk production over an animal’s lifespan. Orchitis, epididymitis and chronic hygroma had been reported in bulls. The current study focused on to studying the detectable immunological responses to Brucella S 19 live attenuated vaccine using serological tests in cattle of dry zone farms. A questionnaire-based survey was carried out in selected farms with and without a previous history of abortion respectively. Animals that were in 1st to 5th lactation in the 18 farms were selected for screening by Rose Bengal Plate Test (RBPT) and positives were confirmed by Complement Fixation Test (CFT). Three farms each with and without history of abortion were selected for Brucella S19 vaccination from the RBPT and CFT negative farms. Sixty non pregnant heifers over 6 months were selected, and 1ml each of S19 vaccine was given subcutaneously to 30 animals while 30 animals were kept as controls. The serum samples were collected and screened for Brucellosis by RBPT and CFT at 2, 4, 6, 8 and 10 weeks of post vaccination. The second week after the vaccination, 71.42% of the animals showed over 1:4 CFT levels while 28.57% of them did not show any antibody titres for Brucellosis in the Brucella endemic farms. In Brucella non-endemic farms, 68.75% of animals showed over 1:4 CFT titre at 2 weeks after the vaccination. However, 31.25% of animals did not show any CFT titre. No persistent and detectable antibody responses were found 10 weeks post vaccination in heifers of endemic and non-endemic herds. The titers of CFT indicate IgG or IgM humeral response, cell mediated immune response of live attenuated vaccine has not been monitored in the study. Thus, seropositivity after the 10 weeks post vaccination may indicate an infection of Brucellosis since no antibodies from vaccination were found. Further investigation with highly sensitive tests such as ELISA is recommended to have a better insight into this aspect in the future.

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Section: Discussionmentioning

confidence: 99%