Characterization of hydrocarbon degrading bacteria isolated from Indian crude oil reservoir and their influence on biocorrosion of carbon steel API 5LX

Abstract

The role of biosurfactants producing hydrocarbon-degrading bacteria (HDB) on biodegradation and bio-corrosion was evaluated. Biodegradation efficiency (BE) of Streptomyces parvus B7 was found to be 82% when compared to other bacteria. Increased production of biosurfactants directly influences the rate of crude oil BE. Corrosion of carbon steel was found to be more severe in mixed bacterial consortia (1.493 ± 0.015 mm/y). X-ray diffraction confirmed the presence of high intensity of ferric oxide (Fe 2 O 3), iron oxide (Fe 3 O 4), manganese oxide (Mn 3 O 4), and manganese dioxide (MnO 2) in corrosion product of mixed bacterial system. Biofilm formation was assist to pit formation on the carbon steel surface and it was evidenced from the atomic force microscopy (AFM) and scanning electron microscopy (SEM) analysis. Corrosion current was increased in the presence of mixed consortia 1.6 ± 0.2 × 10-3 A/cm-2 , compared to abiotic control 1.2 ± 0.15 × 10-4 A/cm-2 , this values were well supported with charge transfer values and these observations confirmed that mixed bacterial consortia play key role in the corrosion of carbon steel. This is the first report to show degradation of crude oil by Streptomyces parvus B7 and its effects on the corrosion of carbon steel in oil reservoir.

Effect of Multispecies Microbial Consortia on Microbially Influenced Corrosion of Carbon Steel

“…There have been varying reports on the effect of the presence of different species of Bacillus on corrosion with some indicating increased corrosion [46][47][48] and others showing corrosion inhibition [49][50][51][52][53]. Both defined mixed microbial treatments, with (T5) or without (T4) D. desulfuricans, had similar levels of general corrosion but qualitatively different biofilm thicknesses, where T5 was greater than T4 (Figures 1 and 2).…”

Marine Bioremediation in Indonesia : Die Before Blossom

“…Mucus that formed from natural polymers that will make the bacteria stick to each other forming the flok so that bacteria can easily be separated from the biological reactor effluents. The production of the polymer is a function of nutrient and growth stages of organisms (Pacwa-Płociniczak et al 2016;Parthipan et al 2018).…”

Antibiofilm effect of mono‐rhamnolipids and di‐rhamnolipids on carbon steel submitted to oil produced water

“…Diverse studies have been carried out evaluating the biofilm effect on metals, including investigations as the influence of degrading bacteria on biocorrosion, 5,8 corrosion behavior in presence of biosurfactant, 9 pit formation, 10 the potential of biosurfactant as biofilm control, 11,12 the effect of rhamnolipids from Pseudomonas aeruginosa on biofilms of sulfate-reducing bacteria, 2 and community composition and diversity of biofilms. 13 Recently, Wood and coauthors confirmed by molecular techniques (by RNA-seq cloning) that rhamnolipids (RML) are the biochemical basis of biofilm dispersal.…”