Characterization of hydroxyapatite and carbonated apatite by photo acoustic FTIR spectroscopy

Rehman, Ihtesham Ur; Bonfield, W.

doi:10.1023/a:1018570213546

Cited by 724 publications

(378 citation statements)

References 3 publications

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“…The weak 1642 band likely arose from SP2 C=C stretch. The inhomogeneous broad peak in the region 3434 cm -1 indicated the presence of exchangeable protons while the homogeneous sharper peaks in the fingerprint region pointed to a possible crystalline nature of an adsorbed compound [24][25][26][27]. The spectra of a neat solution of 20 µM raloxifene without bone powder is shown as reference (Fig.…”

Section: Binding Of Raloxifene To Bone Powdermentioning

confidence: 99%

Structural features underlying raloxifene’s biophysical interaction with bone matrix

Bivi

Balagopalakrishna

et al. 2016

Bioorganic & Medicinal Chemistry

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Raloxifene, a selective estrogen receptor modulator (SERM), reduces fracture risk at least in part by improving the mechanical properties of bone in a cell-and estrogen receptor-independent manner. In this study, we determined that raloxifene directly interacts with the bone tissue. Through the use of multiple and complementary biophysical techniques including nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy (FTIR), we show that raloxifene interacts specifically with the organic component or the organic/mineral composite, and not with hydroxyapatite. Structure-activity studies reveal that the basic side chain of raloxifene is an instrumental determinant in the interaction with bone. Thus, truncation of portions of the side chain reduces bone binding and also diminishes the increase in mechanical properties. Our results support a model wherein the piperidine interacts with bone matrix through electrostatic interactions with the piperidine nitrogen and through hydrophobic interactions (van der Waals) with the aliphatic groups in the side chain and the benzothiopene core. Furthermore, in silico prediction of the potential binding sites on the surface of collagen revealed the presence of a groove with sufficient space to accommodate raloxifene analogs. The hydroxyl groups on the benzothiophene nucleus, which are necessary for binding of SERMs to the estrogen receptor, are not required for binding to the bone surface, but mediate a more robust binding of the compound to the bone powder. In conclusion, we report herein a novel property of raloxifene analogs that allows them to interact with the bone tissue through potential contacts with the organic matrix and in particular collagen.

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Section: Binding Of Raloxifene To Bone Powdermentioning

confidence: 99%

Structural features underlying raloxifene’s biophysical interaction with bone matrix

Bivi

Balagopalakrishna

et al. 2016

Bioorganic & Medicinal Chemistry

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“…The sample exhibited absorption bands due to hydroxyl stretch at *3500 cm -1 (Wang et al 2010). A previous study shows that the hydroxyl group decreases when carbonate content increases (Rehman and Bonfield 1997). The spectrum shows the characteristic of nHA where the intense peak of 1021 cm -1 was associated with PO 4 3-antisymmetric stretching mode (Paz et al 2012).…”

Section: Atr-ftirmentioning

confidence: 79%

“…The peak between 1420 and 1427 cm -1 shown in spectrum, at 1348 cm -1 and another peak at 826 cm -1 , are related to carbonated apatite. The peak at 1560 cm -1 refers to the vibrational mode of carbonate ion (Rehman and Bonfield 1997).…”

Section: Atr-ftirmentioning

confidence: 99%

Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

Hassan

Sultana

2017

3 Biotech

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Considering the important factor of bioactive nanohydoxyapatite (nHA) to enhance osteoconductivity or bone-bonding capacity, nHA was incorporated into an electrospun polycaprolactone (PCL) membrane using electrospinning techniques. The viscosity of the PCL and nHA/PCL with different concentrations of nHA was measured and the morphology of the electrospun membranes was compared using a field emission scanning electron microscopy. The water contact angle of the nanofiber determined the wettability of the membranes of different concentrations. The surface roughness of the electrospun nanofibers fabricated from pure PCL and nHA/PCL was determined and compared using atomic force microscopy. Attenuated total reflectance Fourier transform infrared spectroscopy was used to study the chemical bonding of the composite electrospun nanofibers. Beadless nanofibers were achieved after the incorporation of nHA with a diameter of 200-700 nm. Results showed that the fiber diameter and the surface roughness of electrospun nanofibers were significantly increased after the incorporation of nHA. In contrast, the water contact angle (132°± 3.5°) was reduced for PCL membrane after addition of 10% (w/ w) nHA (112°± 3.0°). Ultimate tensile strengths of PCL membrane and 10% (w/w) nHA/PCL membrane were 25.02 ± 2.3 and 18.5 ± 4.4 MPa. A model drug tetracycline hydrochloride was successfully loaded in the membrane and the membrane demonstrated good antibacterial effects against the growth of bacteria by showing inhibition zone for E. coli (2.53 ± 0.06 cm) and B. cereus (2.87 ± 0.06 cm).

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“…The peak that was observed in the region between 1405 to 1456 cm -1 and 1025.4 cm -1 belongs to the carboxyl -COOH stretching band [16] and C-O stretching vibration [17] respectively where the peaks were detected in pure SA only. The peak V 3 , hydroxyl stretch carbonate and phosphate were observed between 1300 and1650cm -1 [18] 976 and 1190cm -1 [18,19]respectively, where the highest peaks was observed for sample 99CaP which contains 1wt% of SA but the increasing wt% of SA led to a decreasein intensity. The peak for C1-H deformation manunuronic acid residue and hydroxyl stretch phosphate V 4 was observed between 878.9-882.4 cm -1 [17] and 520-600cm -1 [18,20,21] respectively where both of these peaks were observed in sample 97CaP.…”

Section: Fourier Transform Infra-red (Ftir)mentioning

confidence: 94%

“…The peak V 3 , hydroxyl stretch carbonate and phosphate were observed between 1300 and1650cm -1 [18] 976 and 1190cm -1 [18,19]respectively, where the highest peaks was observed for sample 99CaP which contains 1wt% of SA but the increasing wt% of SA led to a decreasein intensity. The peak for C1-H deformation manunuronic acid residue and hydroxyl stretch phosphate V 4 was observed between 878.9-882.4 cm -1 [17] and 520-600cm -1 [18,20,21] respectively where both of these peaks were observed in sample 97CaP. Based on the results,the addition of the SA influenced the intensity peaks of the composite where the formation of chemical bonds occurred between both materials.…”

Section: Fourier Transform Infra-red (Ftir)mentioning

confidence: 94%

Powder Characterization of Calcium Phosphate/Collagen for Bone Implant Application

et al. 2017

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Abstract. Calcium phosphate (CaP) is a type of bioceramic material that is biocompatible and bioactive. It is usually used for bone implant application but it lacks mechanical strength. Therefore, sodium alginate (SA), a natural polymer, is combined with CaP to improve its properties of the CaP via precipitation method. The powder formed is then characterized by using FESEM, EDX, FTIR and DTA. Based on the FESEM result, it was confirmed that the SA particles were well embedded and homogeneously dispersed throughout the CaP matrix whereas the EDX result showed that the CaP and SA are pure and were not contaminated with other materials or substances.The FTIR result showed that the intensity of some peaks (3100-3600 cm-1 and 1585-1625cm-1) increased due to the addition of SA but for some peaks the addition of SA leads to a decrease in intensity (1650-1300cm-1 and 1190-976cm-1).This proves that the addition of SA in CaP influenced the intensity peaks and it was confirmed that chemical bonds were formed between these two substances. Meanwhile, the DTA result showed that CaP dehydroxylation occurred in the range of 650-13000C and the thermal degradation of SA happened at 245.80C but the SA thermal degradation temperature improved when it was added to the CaP matrix.

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Characterization of hydroxyapatite and carbonated apatite by photo acoustic FTIR spectroscopy

Cited by 724 publications

References 3 publications

Structural features underlying raloxifene’s biophysical interaction with bone matrix

Structural features underlying raloxifene’s biophysical interaction with bone matrix

Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

Powder Characterization of Calcium Phosphate/Collagen for Bone Implant Application

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