Characterization of <i>Alpinia officinarum</i> Hance polysaccharide and its immune modulatory activity in mice

Ni, Jinfu; Chen, Huricha; Zhang, Chenglong; Luo, Qiyuan; Qin, Yao; Yang, Yuhui; Chen, Yun

doi:10.1039/d1fo03949k

Cited by 15 publications

(6 citation statements)

References 34 publications

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“…Notably, the AOPs were rich in glucose, especially AOP30 (89.88%). This result is consistent with that of a previous study [30], whereas the level of galactose in AOP50 and AOP70 was eight times higher than that found in AOP30.…”

Section: Molecular Weights Monosaccharide Compositions and Zeta Poten...supporting

confidence: 93%

Ultrasonic-Assisted Extraction, Structural Characteristics, and Antioxidant Activities of Polysaccharides from Alpinia officinarum Hance

Jia,

Liu,

Huang

et al. 2024

Foods

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Alpinia officinarum Hance, a well known agricultural product in the Lei Zhou peninsula, is generally rich in polysaccharides. In order to enhance the use of A. officinarum Hance polysaccharides (AOP) in functional food, AOP was extracted using an ultrasonic-assisted extraction method, and the ultrasonic extraction parameters of AOP was optimized. Furthermore, this study investigated the physicochemical and antioxidant activities of AOPs. In addition, the structural properties were preliminarily determined using Fourier-transform infrared spectroscopy (FTIR), high performance size exclusion chromatography, and a Zetasizer. Ultimately, this study explored the mechanism underlying the antioxidant activities of AOP. The results showed that the optimal ultrasonic-assisted extraction parameters were as follows: ultrasonic time, 6 min; ratio of water to material, 12 mL/g; and ultrasonic power, 380 W. Under these conditions, the maximum yield of AOPs was 5.72%, indicating that ultrasonic-assisted extraction technology is suitable for extracting AOPs due to the reduced time and water usage. Additionally, AOPs were purified using graded alcohol precipitation, resulting in three fractions (AOP30, AOP50, and AOP70). AOP30 had the lowest molecular weight of 11.07 kDa and mainly consisted of glucose (89.88%). The half inhibitory concentration (IC50) value of AOP30 and AOP70 was lower than that of AOP50 in the ability to scavenge the ABTS radical, while a reverse trend was observed in reducing ferric ions. Notably, the antioxidant activities of AOPs were highly correlated with their polydispersity index (Mw/Mn) and Zeta potential. AOP30, a negatively charged acidic polysaccharide fraction, exhibited electron donating capacities. Additionally, it displayed strong antioxidant abilities through scavenging 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radicals and reducing ferric ions. In conclusion, the present study suggests that AOP30 could be developed as an antioxidant ingredient for the food industry.

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Section: Molecular Weights Monosaccharide Compositions and Zeta Poten...supporting

confidence: 93%

Ultrasonic-Assisted Extraction, Structural Characteristics, and Antioxidant Activities of Polysaccharides from Alpinia officinarum Hance

Jia,

Liu,

Huang

et al. 2024

Foods

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“…Compared with SCCP, the prominent absorption peaks did not change remarkably in the polysaccharide-iron (III) complexes prepared at four different temperatures. The absorption peak near 3340 cm −1 was mainly caused by the −OH stretching vibration, and the absorption peak near 1650 cm −1 was mainly due to the C=O asymmetric vibration of −COOH in the polysaccharide [ 21 ]. It also shows that the absorption peak around 1400 cm −1 reflects the symmetric vibration of C−O in −COOH.…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization, In Vitro Digestion Property, and Biological Activity of Sweet Corn Cob Polysaccharide Iron (III) Complexes

Xiu

Wang

et al. 2023

Molecules

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This study aimed to enhance the utilization value of sweet corn cob, an agricultural cereal byproduct. Sweet corn cob polysaccharide-ron (III) complexes were prepared at four different temperatures (40 °C, 50 °C, 60 °C, and 70 °C). It was demonstrated that the complexes prepared at different temperatures were successfully bound to iron (III), and there was no significant difference in chemical composition; and SCCP-Fe-C demonstrated the highest iron content. The structural characterization suggested that sweet corn cob polysaccharide (SCCP) formed stable β-FeOOH iron nuclei with −OH and −OOH. All the four complexes’ thermal stability was enhanced, especially in SCCP-Fe-C. In vitro iron (III) release experiments revealed that all four complexes were rapidly released and acted as iron (III) supplements. Moreover, in vitro antioxidant, α-glucosidase, and α-amylase inhibition studies revealed that the biological activities of all four complexes were enhanced compared with those of SCCP. SCCP-Fe-B and SCCP-Fe-C exhibited the highest in vitro antioxidant, α-glucosidase, and α-amylase inhibition abilities. This study will suggest using sweet corn cobs, a natural agricultural cereal byproduct, in functional foods. Furthermore, we proposed that the complexes prepared from agricultural byproducts can be used as a potential iron supplement.

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“…The AHP-3a monosaccharide composition was determined as per the method suggested by Ni et al [21]. Briefly, AHP-3a (5 mg) was hydrolyzed using 3 mol/L TFA at 120 • C for 3 h. The hydrolyzed product was dried under a nitrogen-filled atmosphere, washed with methanol to remove residual TFA, and dissolved in water.…”

Section: Chemical and Monosaccharide Composition Analysesmentioning

confidence: 99%

“…On investigating the biological activity of the A. officinarum crude polysaccharide, Hailiwu et al found that the polysaccharide could successfully suppress the growth of the murine forestomach carcinoma cell line (MFC) stomach cancer xenograft in mice [20]. Following a comprehensive structural examination, Ni et al reported that the neutral polysaccharide of A. officinarum possesses immunomodulatory properties [21]. Most of these past reports are focused on A. officinarum crude and neutral polysaccharides, and there have been no studies on the structural properties and biological activities of A. officinarum acidic polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Characterization, Antioxidant and Anticancer Activity Evaluation of an Acidic Polysaccharide from Alpinia officinarum Hance

Wen,

Kuang,

Lian

et al. 2024

Molecules

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AHP-3a, a triple-helix acidic polysaccharide isolated from Alpinia officinarum Hance, was evaluated for its anticancer and antioxidant activities. The physicochemical properties and structure of AHP-3a were investigated through gel permeation chromatography, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. The weight-average molecular weight of AHP-3a was 484 kDa, with the molar percentages of GalA, Gal, Ara, Xyl, Rha, Glc, GlcA, and Fuc being 35.4%, 21.4%, 16.9%, 11.8%, 8.9%, 3.1%, 2.0%, and 0.5%, respectively. Based on the results of the monosaccharide composition analysis, methylation analysis, and NMR spectroscopy, the main chain of AHP-3a was presumed to consist of (1→4)-α-D-GalpA and (1→2)-α-L-Rhap residues, which is a pectic polysaccharide with homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) structural domains containing side chains. In addition, the results of the antioxidant activity assay revealed that the ability of AHP-3a to scavenge DPPH, ABTS, and OH free radicals increased with an increase in its concentration. Moreover, according to the results from the EdU, wound healing, and Transwell assays, AHP-3a can control the proliferation, migration, and invasion of HepG2 and Huh7 hepatocellular carcinoma cells without causing any damage to healthy cells. Thus, AHP-3a may be a natural antioxidant and anticancer component.

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Characterization of Alpinia officinarum Hance polysaccharide and its immune modulatory activity in mice

Abstract: This study aimed to verify the regulating effect of a novel water-soluble polysaccharide (AOHP) extracted from Alpinia officinarum Hance on mouse immunity and to characterize its structure feature. The cellulose...

Cited by 15 publications

References 34 publications

Ultrasonic-Assisted Extraction, Structural Characteristics, and Antioxidant Activities of Polysaccharides from Alpinia officinarum Hance

Ultrasonic-Assisted Extraction, Structural Characteristics, and Antioxidant Activities of Polysaccharides from Alpinia officinarum Hance

Structural Characterization, In Vitro Digestion Property, and Biological Activity of Sweet Corn Cob Polysaccharide Iron (III) Complexes

Physicochemical Characterization, Antioxidant and Anticancer Activity Evaluation of an Acidic Polysaccharide from Alpinia officinarum Hance

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