Characterization ofBacillus subtilisHC8, a novel plant‐beneficial endophytic strain from giant hogweed

Abstract

SummaryThirty endophytic bacteria were isolated from various plant species growing near Saint‐Petersburg, Russia. Based on a screening for various traits, including plant‐beneficial properties and DNA fragment patterns, potential siblings were removed. The remaining isolates were taxonomically identified using 16S rDNA sequences and potential human and plant pathogens were removed. The remaining strains were tested for their ability to promote radish root growth and to protect tomato plants against tomato foot and root rot. One strain, Bacillus subtilis HC8, isolated from the giant hogweed Heracleum sosnowskyi Manden, significantly promoted plant growth and protected tomato against tomato foot and root rot. Metabolites possibly responsible for these plant‐beneficial properties were identified as the hormone gibberellin and (lipo)peptide antibiotics respectively. The antibiotic properties of strain HC8 are similar to those of the commercially available plant‐beneficial strain Bacillus amyloliquefaciens FZB42. However, thin layer chromatography profiles of the two strains differ. It is speculated that endophytes such as B. subtilis HC8 contribute to the fast growth of giant hogweed.

Colonization by endophytic Ochrobactrum anthropi Mn1 promotes growth of Jerusalem artichoke

“…Endophytic bacteria may promote plant growth through nitrogen fixation (Gyaneshwar et al ., ; Paungfoo‐Lonhienne et al ., ), phytohormone production (Chen et al ., ; Malfanova et al ., ) and enhanced uptake of mineral nutrients (Rajendran et al ., ; Weyens et al ., ). In our study, the O. anthropi Mn1 strain fixed nitrogen in association with the host plant when colonized in root and stem tissues.…”

Plant defense approach ofBacillus subtilis(BERA 71) againstMacrophomina phaseolina(Tassi) Goid in mung bean

“…The precipitate was extracted twice with methanol and concentrated by vacuum evaporation, and re-dissolved in known volume of methanol and stored at −20°C. The methanolic extracts were analyzed by thin layer chromatography (TLC) on silica gel 60 F 254 plates with layer thickness of 250 µm (Merck, Darmstadt, Germany) as described by Malfanova et al (2011). Plates were developed in chloroform/methanol/water 65:25:4 (v/ v/v) for 1.5 h at room temperature.…”

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

“…In addition to its role in protecting plants against pathogens, the chitinolytic bacterium B. subtilis AF 1 was found to promote seed germination and subsequent plant growth in pigeon pea even under pathogen pressure [64] with this response amplified by the addition of chitin to the carrier medium [179]. As B. subtilis is known utilize chitin [179] and strains of this bacteria are known to produce plant hormones (auxins) [180] the promotion in germination could potentially be due to changes in plant hormone levels in the rhizosphere.…”