Characterization of
 ermB
 Gene Transposition by Tn
 1545
 and Tn
 917
 in Macrolide-Resistant
 Streptococcus pneumoniae
 Isolates

Okitsu, Naohiro; Kaieda, Satoru; Yano, Hisakazu; Nakano, Ryuichi; Hosaka, Yoshio; Okamoto, Ryouichi; Kobayashi, Toshimitsu; Inoue, Matsuhisa

doi:10.1128/jcm.43.1.168-173.2005

Cited by 48 publications

(21 citation statements)

References 22 publications

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“…46 For MLSB resistance genes, ermB gene was enriched 126.5-fold (compared with control soil sample), and ermB gene has been found on transposons, such as Tn917 and Tn1545. 47 Plainly, those ARGs which are associated with MGEs can move from one species to another through horizontal gene tranfer mechanisms, 34 with easy dissemination and obstinate persistance in various environments.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

Wang

Qiao

et al. 2014

Environ. Sci. Technol.

393

201

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Reclaimed water irrigation (RWI) in urban environments is becoming popular, due to rapid urbanization and water shortage. The continuous release of residual antibiotics and antibiotic resistance genes (ARGs) from reclaimed water could result in the dissemination of ARGs in the downstream environment. This study provides a comprehensive profile of ARGs in park soils exposed to RWI through a high-throughput quantitative PCR approach. 147 ARGs encoding for resistance to a broad-spectrum of antibiotics were detected among all park soil samples. Aminoglycoside and beta-lactam were the two most dominant types of ARGs, and antibiotic deactivation and efflux pump were the two most dominant mechanisms in these RWI samples. The total enrichment of ARGs varied from 99.3-fold to 8655.3-fold compared to respective controls. Six to 60 ARGs were statistically enriched among these RWI samples. Four transposase genes were detected in RWI samples. TnpA-04 was the most enriched transposase gene with an enrichment was up to 2501.3-fold in Urumqi RWI samples compared with control soil samples. Furthermore, significantly positive correlation was found between ARGs and transposase abundances, indicating that transposase might be involved in the propagation of ARGs. This study demonstrated that RWI resulted in the enrichment of ARGs in urban park soils.

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Section: ■ Results and Discussionmentioning

confidence: 99%

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

Wang

Qiao

et al. 2014

Environ. Sci. Technol.

393

201

View full text Add to dashboard Cite

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“…There are some geographical differences in the prevalence and spread of transposons carried erm (B) gene in pneumococci. In Italy and Spain, the most frequently reported transposons in S. pneumoniae are Tn 6002 and Tn 3872 , whereas in Japan Tn 917 was found to be the most common [ 14 , 23 , 24 , 33 ]. Tn 3872 is a composite, mobile element resulting from the insertion of the erm -containing Tn 917 transposon into Tn 916 [ 8 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

Resistance Determinants and Their Association with Different Transposons in the Antibiotic-ResistantStreptococcus pneumoniae

Korona-Głowniak

Siwiec

Malm

2015

BioMed Research International

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Multiple resistance of Streptococcus pneumoniae is generally associated with their unique recombination-mediated genetic plasticity and possessing the mobile genetic elements. The aim of our study was to detect antibiotic resistance determinants and conjugative transposons in 138 antibiotic-resistant pneumococcal strains isolated from nasopharynx of healthy young children from Lublin, Poland. These strains resistant to tetracycline and/or to chloramphenicol/erythromycin/clindamycin were tested by PCR using the specific genes as markers. The presence of Tn916 family transposons, carrying tet(M) and int/xisTn916, was observed in all of the tested strains. Tn916 was detected in 16 strains resistant only to tetracycline. Tn6002 and Tn3872-related element were found among 99 erm(B)-carrying strains (83.8% and 3.0%, resp.). Eight strains harbouring mef(E) and erm(B) genes were detected, suggesting the presence of Tn2010 and Tn2017 transposons. Among 101 chloramphenicol-resistant strains, two variants of Tn5252-related transposon were distinguished depending on the presence of int/xis5252 genes specific for cat gene-containing Tn5252 (75.2% of strains) or int Sp23FST81 gene, specific for cat-containing ICESp23FST81 element (24.8% of strains). In 6 strains Tn916-like and Tn5252-like elements formed a Tn5253-like structure. Besides clonal dissemination of resistant strains of pneumococci in the population, horizontal transfer of conjugative transposons is an important factor of the high prevalence of antibiotic resistance.

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“…The macrolide resistance gene ermB was also found to be abundant in samples across all broiler sheds. Similar to the tet genes, ermB was also detected in a wide range of bacterial species [29] and associated with conjugative transposons [36][37][38], which may explain the wide dissemination of the gene in the environment.…”

Section: Discussionmentioning

confidence: 93%

Antibiotic Resistance Genes in Antibiotic-Free Chicken Farms

et al. 2020

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Rising concern about the use of antibiotics in food production has resulted in many studies on the occurrence of antibiotic resistance genes (ARGs) in animal-associated bacterial communities. There are few baseline data on the abundance of ARGs on farms where chickens are intensively raised with little or no use of antibiotics. This study used a high-throughput quantitative PCR array to survey two antibiotic-free chicken farms for the occurrence of ARGs and mobile genetic elements known to enhance the spread of ARGs. No antibiotics had been used on the study farms for five years prior to this study. The results provide a baseline for the occurrence of resistance genes in the chicken production system without direct selective pressure.Antibiotics 2020, 9, 120 2 of 12 cost-effective and efficient method to collect biomass representing a large population, and this has been used for monitoring the prevalence of pathogens in chicken flocks [8,9]. High-throughput quantitative PCR (HT-qPCR) and deep sequencing approaches have been used in a variety of human, animal and environmental samples to characterize the diversity of antibiotic resistance genes (ARGs) [10][11][12][13][14]. These methods are efficient and effective for the broad-spectrum detection and quantification of ARGs in complex samples.The aim of this study was to assess the presence and diversity of ARGs and mobile genetic elements (MGEs) in poultry farms that had not used antibiotics for 5 years. The WaferGen HT-qPCR system was used to detect and quantify ARGs and MGEs in environmental samples from two chicken farms. The PCR primers targeted ARGs of the major antibiotic classes, and integrases and transposase genes. Caged chicken sheds from two types of farms were selected for investigation; the first was an egg production enterprise which housed caged layer chickens (Farm L), while the second was a broiler breeder farm (Farm B). The results should provide useful baseline information regarding ARG prevalence in the chicken production system without direct selective pressure.

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Characterization of ermB Gene Transposition by Tn 1545 and Tn 917 in Macrolide-Resistant Streptococcus pneumoniae Isolates

Cited by 48 publications

References 22 publications

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

High Throughput Profiling of Antibiotic Resistance Genes in Urban Park Soils with Reclaimed Water Irrigation

Resistance Determinants and Their Association with Different Transposons in the Antibiotic-ResistantStreptococcus pneumoniae

Antibiotic Resistance Genes in Antibiotic-Free Chicken Farms

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