Characterization ofin vitrometabolites of luotonin A in human liver microsomes using electrospray/tandem mass spectrometry

Abstract

1. The pharmacological activity of luotonin A varies, depending on the type of functional group and the site of derivatization. To understand the in vivo efficacy of luotonin A, the in vitro metabolism of luotonin A was investigated in human liver microsomes and recombinant cDNA-expressed cytochromeP450 (CYP). 2. Incubation of luotonin A with pooled human liver microsomes in the presence of NADPH-generating system resulted in the formation of four metabolites and the structures of each metabolite were tentatively characterized on the basis of electrospray tandem mass spectra. 3. The main metabolic pathway of luotonin A in human liver microsomes was hydroxylation, resulting in the generation of two mono-hydroxyl metabolites (M1 and M2) and two di-hydroxyl metabolites (M3 and M4). CYP1A2 was primarily involved in hydroxylation of the quinolone moiety (M1 and M3), while CYP3A4 was mainly responsible for hydroxylation of the quinazoline moiety of luotonin A (M2 and M4) in human liver microsomes.

Challenging core-shell stationary phases with the separation of closely related anti-cancer compounds: performance studies and application to drug quantitation in cell cultures with multi-well plate clean-up

“…Furthermore, the use of commercially available chiral stationary phases or the inverted chirality columns approach, has made possible the chiral resolution of enantiomers of the drugs [21]. Luotonin A derivatives also exhibit a strong fluorescence [22,23], although the literature about the separation of luotonin A analogues is very scarce [24,25]. We have previously reported a methodology for the quantification of luotonin A derivatives in human serum samples [24].…”