Characterization of Komagataeibacter xylinus by a polarization modulation imaging method

Abstract

Komagataeibacter xylinus (K. xylinus) has been used for a long time as one of the main cellulose producers among bacterium. In order to gain a better understanding of the physiological and biochemical mechanisms of cellulose production, an efficient and noninvasive visualization method is highly demanded to monitor the morphological changes of K. xylinus during each stage of its development. In this study, a polarization parametric indirect microscopic imaging (PIMI) technique was applied to image the morphology variations of K. xylinus. The optical field with precisely controlled polarization status was incident on the sample and far-field images of the sample were taken under different illumination polarization. In the calculated parametric images, sub-diffraction features related to the morphology details of the K. xylinus were visualized with high contrast and spatial resolution, which is typically irresolvable in conventional microscopic images. Finite-difference time-domain simulations verified the visualized scattering distributions in PIMI imaging results. We believe these pieces of evidence proved the possibility of developing a platform for characterizing the structures and morphological changes of cells in the cultivating process.

Polarization multi-parametric imaging method for the inspection of cervix cell

“…Recently, it has been demonstrated that the PIMI method, with its multi-parametric images, can be used to obtain rich detailed features of sub-cellular structures [27]. By measuring and filtering the distribution of the far-field optical scattering, PIMI can reveal high resolution nanoscale anisotropic structures using precisely modulated incident polarization [28,29].…”

Gold-viral particle identification by deep learning in wide-field photon scattering parametric images

“…In our previous work, we have investigated the ability of polarization indirect microscopic imaging (PIMI) to resolve nano-features and molecular structures from the spatial polarization status distribution in the nano scattering field [15][16][17][18]. We also demonstrated that anisotropy in nanostructures can be identified from the scattered photon distribution [19,20]. Moreover, the PIMI scattering strength of viruses can be magnified by introducing an abrupt change of refractivity at the virus particle, enhancing the signal to the point where the presence of virus can be identified [21].…”

Photon Scattering Signal Amplification in Gold-Viral Particle Ligation Towards Fast Infection Screening

“…In our previously published papers, we have investigated the capability of polarization indirect microscopic imaging (PIMI) in resolving nano-features and molecular structures from the spatial polarization status distribution in the nano scattering field [19,20]. It is found that the polarization states of the photons can be used to map or image the anisotropic features of the nanostructure, with a resolving power beyond the diffraction limit of conventional microscopy, by detecting the variation of coupling and scattering photon states from the sample [21,22]. However, the scattering strength heavily depends on the change in refractivity in the near field under measurement.…”