Characterization of <i>LcGAPC</i> and its transcriptional response to salt and alkali stress in two ecotypes of <i>Leymus chinensis</i> (Trin.) Tzvelev

Lu, Qian; Meng, Xue; Yang, Fangming; Liu, Xin; Cui, Jizhe

doi:10.1080/13102818.2020.1719020

Cited by 8 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, these genes might have similar functions. GapC-type genes have been shown to participate in plant responses to heat, anaerobic conditions, salt and drought (Yang et al 1993;Lu et al, 2020;Kappachery et al 2015). Other studies also demonstrated that GapC-type genes play important roles in helping plants withstand abiotic stresses.…”

Section: Discussionmentioning

confidence: 99%

“…ThGAPB can reduce the production of ROS by maintaining a high recycling rate of ADP and NADP + , helpful for the maintenance of photosynthetic efficiency and development of plants under saline conditions, as it can improve the salt tolerance of plants (Chang et al 2015). The LcGAPC gene is highly expressed in the two ecotypes of Leymus chinensis under longterm and high-concentration NaCl stress (Lu et al 2020). PsGAPDH from oyster mushroom has been transferred into rice, and seedlings of this transgenic rice are significantly better than the wild type in growth and germination under salt stress (Lim et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…The LcGAPC gene is highly expressed in the two ecotypes of Leymus chinensis under long‐term and high‐concentration NaCl stress (Lu et al . 2020). PsGAPDH from oyster mushroom has been transferred into rice, and seedlings of this transgenic rice are significantly better than the wild type in growth and germination under salt stress (Lim et al .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of the GAPDH gene family in Citrullus lanatus and functional characteristics of ClGAPC2 in Arabidopsis thaliana

Sun

Wang

et al. 2022

Plant Biol J

View full text Add to dashboard Cite

Members of the GAPDH family play important roles in plant growth and development, as well as in stress responses. Our aim was to identify stress resistance genes through systematic analysis of the GAPDH family in watermelon. This could not only provide genetic resources for stress resistance breeding, but also form a basis for the study of plant stress resistance mechanisms. Eight GAPDHs representing four types of plant GAPDH in watermelon were identified (ClGAPA/B, ClGAPC1‐3, ClGAPCp1‐2 and ClGAPN). A comprehensive analysis of physicochemical properties, chromosome distribution, evolutionary relationships, exon‐intron structure and conserved motifs of watermelon GAPDHs was performed using bioinformatics. Expression characteristics were assessed by RT‐qPCR. Based on RT‐qPCR results, ClGAPC2 was screened as a candidate for subcellular localization analysis and functional verification in Arabidopsis thaliana. Eight GAPDHs were classified into four subfamilies. GAPDHs in each subgroup were generally conserved and shared similarities in structure and conserved motifs. ClGAPDHs had notable tissue specificity and different expression patterns in response to H2O2, chilling, salt, osmotic stress, heat, salicylic acid, gibberellin, brassinosterol, ethylene and abscisic acid treatments. Three ClGAPC genes, especially ClGAPC2, were markedly induced by several treatments. ClGAPC2 was located in the nucleus and cytoplasm of tabacum epidermal cells. The ClGAPC2 transgenic Arabidopsis showed enhanced tolerance to salinity at the germination stage. We suggest that ClGAPC2 plays important roles in the adaptation of watermelon to salinity. Our findings provided candidate genes for further improving the salt tolerance of watermelon.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of the GAPDH gene family in Citrullus lanatus and functional characteristics of ClGAPC2 in Arabidopsis thaliana

Sun

Wang

et al. 2022

Plant Biol J

View full text Add to dashboard Cite

show abstract

“…It was previously reported that the overexpression of HXK1 increases tolerance by regulating NHX1 gene expression to salt stress in Malus domestica , similarly, GAPC helps in NaCl stress tolerance by acting as sensor for H 2 O 2 in Leymus chinensis [ 126 , 127 ]. Reports showed that the carbohydrates are not only used to adjust the osmotic balance but also play an important role as protective agents in plants to regulate homeostasis under salt stress [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Investigation of an Antioxidative System for Salinity Tolerance in Oenanthe javanica

Kumar

Yang

et al. 2020

Antioxidants

View full text Add to dashboard Cite

Abiotic stress, such as drought and salinity, severely affect the growth and yield of many plants. Oenanthe javanica (commonly known as water dropwort) is an important vegetable that is grown in the saline-alkali soils of East Asia, where salinity is the limiting environmental factor. To study the defense mechanism of salt stress responses in water dropwort, we studied two water dropwort cultivars, V11E0022 and V11E0135, based on phenotypic and physiological indexes. We found that V11E0022 were tolerant to salt stress, as a result of good antioxidant defense system in the form of osmolyte (proline), antioxidants (polyphenols and flavonoids), and antioxidant enzymes (APX and CAT), which provided novel insights for salt-tolerant mechanisms. Then, a comparative transcriptomic analysis was conducted, and Gene Ontology (GO) analysis revealed that differentially expressed genes (DEGs) involved in the carbohydrate metabolic process could reduce oxidative stress and enhance energy production that can help in adaptation against salt stress. Similarly, lipid metabolic processes can also enhance tolerance against salt stress by reducing the transpiration rate, H2O2, and oxidative stress. Furthermore, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that DEGs involved in hormone signals transduction pathway promoted the activities of antioxidant enzymes and reduced oxidative stress; likewise, arginine and proline metabolism, and flavonoid pathways also stimulated the biosynthesis of proline and flavonoids, respectively, in response to salt stress. Moreover, transcription factors (TFs) were also identified, which play an important role in salt stress tolerance of water dropwort. The finding of this study will be helpful for crop improvement under salt stress.

show abstract

“…Previous studies have shown that there are two ecotypes of L. chinensis, namely, gray-green (GG) and yellow-green (YG), which differ in their survival strategies. GG has a wider distribution and is more resistant to salt stress compared with YG (Zhou et al, 2014;Lu et al, 2020). Thus, differences in L. chinensis ecotypes may reflect adaptive evolution (Kawecki and Ebert, 2004;Savolainen et al, 2007;Delker et al, 2010;Liu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Differences in the Seed Germination of Leymus chinensis (Poaceae) Ecotypes Reveal Distinct Strategies for Coping With Salinity Stress: A Common Garden Experiment

Ma¹,

Ma²,

Wang³

et al. 2021

Front. Ecol. Evol.

View full text Add to dashboard Cite

Soil salinity is important abiotic stress affecting various ecosystems worldwide such as grassland. Distinct ecotypes often evolve within species by natural selection to facilitate adaptation to different types of environmental stress. Leymus chinensis is a perennial rhizomatous grass that is widely distributed in the eastern Eurasian steppe; it has two main ecotypes, namely, yellow-green (YG) and gray-green (GG), which differ in their strategy for coping with salinity stress. Few studies have examined the seed germination of the two ecotypes under salinity stress. In this study, the seed germination and seedling growth of two ecotypes of L. chinensis in response to different levels of salinity (NaCl) stress [0 (control), 20, 50, 100, and 200 mM] were examined. Then, ungerminated seeds were placed under normal conditions to evaluate seedling growth following exposure to salt stress (i.e., regermination). The germination percentage was significantly higher, and the mean germination time was significantly shorter in the GG ecotype than in the YG ecotype at all NaCl concentrations. As the salinity level increased, the radicle length of the two ecotypes decreased; however, GG had longer radicles and a higher number of radicles, even at 200 mM NaCl when no radicle protruding from the seed coat was detected in YG. The shoot length of GG was significantly longer than that of YG at all NaCl levels. After salinity stress was removed, the seed germination percentage increased as the original concentration of NaCl applied increased, but the total germination percentage did not significantly differ among NaCl concentrations. The total seed germination percentage of GG was approximately 80%, whereas that of the YG was approximately 20%. The seedling length of regerminated seeds for both GG and YG was similar. The thousand-grain weight of GG was significantly higher than that of YG. GG was more salt-tolerant than YG and might be better capable of surviving in harsher environments, suggesting that GG might be particularly useful for saline grassland restoration.

show abstract

Characterization of LcGAPC and its transcriptional response to salt and alkali stress in two ecotypes of Leymus chinensis (Trin.) Tzvelev

Cited by 8 publications

References 27 publications

Identification of the GAPDH gene family in Citrullus lanatus and functional characteristics of ClGAPC2 in Arabidopsis thaliana

Identification of the GAPDH gene family in Citrullus lanatus and functional characteristics of ClGAPC2 in Arabidopsis thaliana

Investigation of an Antioxidative System for Salinity Tolerance in Oenanthe javanica

Differences in the Seed Germination of Leymus chinensis (Poaceae) Ecotypes Reveal Distinct Strategies for Coping With Salinity Stress: A Common Garden Experiment

Contact Info

Product

Resources

About