Characterization of Listeria monocytogenes from three countries and antibiotic resistance differences among countries and Listeria monocytogenes serogroups

Abstract

A total of 104 Listeria monocytogenes isolates from 330 fish samples from three countries were characterized by multiplex PCR for serogrouping and virulence markers determination and tested for antibiotics resistance. A 53·8% of the isolates belonged to serogroup 1/2a, 3a; 32% belonged to 1/2b, 3b, 7; 14·4% belonged to 4b, 4d, 4e and 1% belonged to 1/2c, 3c. All isolates exhibited resistance to at least one antibiotic but the resistance rates varied among countries. The isolates exhibited high resistance to penicillin, rifampicin, clindamycin, erythromycin and tetracycline, but low resistance to amoxicillin-clavulanic acid, streptomycin, sulfamethoxazole-trimethoprim, gentamicin, chloramphenicol and kanamycin. When comparing countries, the resistance rate for rifampicin, clindamycin, erythromycin, tetracycline, amoxicillin-clavulanic acid varied among countries. When comparing serogroup, 1/2a, 3a exhibited the highest resistance to clindamycin, erythromycin, tetracycline and vancomycin while serogroup 4b, 4d, 4e exhibited the highest resistance to amoxicillin-clavulanic acid. All isolates carried inlA, inlC, inlJ and lmo2672. Listeriolysin S was carried by 42 and 30% of 4b and 1/2b isolates respectively. Significance and impact of the study: This is one of few studies to correlate antibiotic resistance with Listeria monocytogenes serogroups. The study also compared the antibiotic resistance and serogroups of L. monocytogenes isolates from three countries in one single study. The findings of this study will be helpful in improving data on the antibiotics resistance of L. monocytogenes in developing countries and enriches the epidemiological and public health studies of L. monocytogenes.

Prevalence and molecular characterization of Listeria spp. and Listeria monocytogenes isolated from fish, shrimp, and cooked ready-to-eat (RTE) aquatic products in Iran

“…Our results revealed that the serotypes of lineage II (1/2a, 1/2c, 3a) are most frequently present in clinical and seafood isolates. Similar findings have also been reported elsewhere (Ebner et al, 2015;Kanki, Naruse, Taguchi, & Kumeda, 2015;Leong, Alvarez-Ord oñez, & Jordan, 2014;Miya et al, 2010;Obaidat, Bani Salman, Lafi, & Al-Abboodi, 2015;Vitas, Garcia-Jalon, & Aguado, 2004).…”

Virulence factors and resistance to antimicrobials inListeria monocytogenesserotype 1/2c isolated from food

“…), as well as high levels of resistance to penicillin, erythromycin and vancomycin when recovered from fish sources (Obaidat et al . ). This could be due to not only uncontrolled use of antimicrobials in the given area but also evaluation criteria applied in the studies.…”

“…In particular, L. monocytogenes isolates recovered from foods (Jamali et al 2015) have been reported to have high levels of resistance to ampicillin (20Á9%) and penicillin (16Á3%). Studies showed resistance to penicillin when isolates from dairy foods were tested (90%) (Harakeh et al 2009), as well as high levels of resistance to penicillin, erythromycin and vancomycin when recovered from fish sources (Obaidat et al 2015). This could be due to not only uncontrolled use of antimicrobials in the given area but also evaluation criteria applied in the studies.…”

“…However, the occurrence of L. monocytogenes strains resistant to different antimicrobials, including those used in listeriosis treatment, has been documented in human population, food and the environment of food processing plants. In particular, these strains possessed resistance to penicillin, ampicillin, vancomycin, trimethoprim and tetracycline (Conter et al 2009;Morvan et al 2010;Granier et al 2011;Jamali et al 2015;Obaidat et al 2015).…”

Characterization of antibiogram fingerprints in Listeria monocytogenes recovered from irrigation water and agricultural soil samples

“…A sterile swab was used to inoculate the mixture evenly on Mueller-Hinton agar and antibiotics transferred on the agar using a disc dispenser after which they were incubated for 24 hours at 37˚C. The inhibition zones were measured to the nearest mm and the values were interpreted as "Resistant (R), Intermediate (I) or Susceptible (S)" using the standards recommended by CLSI [37] for Staphylococci [38].…”