Characterization ofRhizobium tropiciClAT899 Nodulation Factors: The Role ofnodHandnodPQGenes in Their Sulfation

Jl, Folch-Mallol; Marroquı́, Silvia; Sousa, Carolina; Manyani, Hamid; Im, López-Lara; Km, van der Drift; Haverkamp, Johan; Quinto, Carmen; Gil‐Serrano, Antonio; Thomas‐Oates, Jane; Hp, Spaink; Megı́as, Manuel

doi:10.1094/mpmi-9-0151

Cited by 69 publications

(8 citation statements)

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“…raddiana in Africa are identical or very close to Nod factors produced by other fast-growing Acacia nodulating rhizobia i.e. Sinorhizobium terangae ORS1073 and M. plurifarium ORS1001 isolated from A. senegal (Lorquin et al, 1997), strain GRH2 isolated from Acacia cyanophylla (LopezLara et al, 1995) and Rhizobium tropici CFN299 and CIAT899 (Folch-Mallol et al, 1996) isolated from bean. Since, as A. senegal appears to have strict requirements in regard to Nod factors the rhizobia that nodulate it produce (Ba et al, 2002), the species seems to be mainly nodulated by a type of rhizobia according to the geographic position.…”

Section: Phylogeny Of Igs 16s-23s Rrnamentioning

confidence: 65%

Distribution and Diversity of Rhizobial Populations Associated with Acacia senegal (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Bakhoum¹,

Roux²,

Diouf³

et al. 2014

OJF

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Distribution and diversity of rhizobial strains associated with Acacia senegal (L.) Willd. in relation to seed provenances in soils from arid (Dahra) and semiarid (Goudiry) zones of Senegal were investigated. PCR-RFLP performed on 16S-23S rDNA intergenic spacer (IGS) of nodule crude extracts revealed a high genetic diversity of rhizobial strains, which was higher in the semiarid region than in the arid region. The distribution of rhizobial populations was influenced by soil physical and chemical characteristics, and by A. senegal provenances as shown by the analysis of correspondence. In contrast, the phenotypic diversity of rhizobial strains was not correlated with the soil origin. The phylogenetic tree (performed by the maximum likelihood algorithm) of IGS 16S-23S sequences showed that most of the rhizobial strains nodulating A. senegal were closely related to Mesorhizobium plurifarium. Our results showed that rhizobial taxa associated with A. senegal were mainly distributed according to soil physical and chemical characteristics, and A. senegal provenances. A large subset of A. senegal root-nodulating bacteria had high diversity that correlated with the most favourable environmental conditions. Understanding the diversity and distribution of rhizobial strains may be exploited in the formulation of A. senegal inoculants for different seed provenances for resilience to soil stresses in various environmental conditions.

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Section: Phylogeny Of Igs 16s-23s Rrnamentioning

confidence: 65%

Distribution and Diversity of Rhizobial Populations Associated with Acacia senegal (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Bakhoum¹,

Roux²,

Diouf³

et al. 2014

OJF

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“…Using different techniques, similar Nod factor structures have been attributed to CFN 299 [48], CIAT 899 [49] and PRF 81 [15]. The tropici pSym gene cluster nodD-ABCSUIJ-HPQ , described in previous studies [49,50], is able to direct the synthesis of Nod factors and to confer nodulation ability when transferred to a non-nodulating strain [51].…”

Section: Resultsmentioning

confidence: 91%

“…The tropici pSym gene cluster nodD-ABCSUIJ-HPQ , described in previous studies [49,50], is able to direct the synthesis of Nod factors and to confer nodulation ability when transferred to a non-nodulating strain [51]. nodC directs the synthesis of the Nod factor-backbone chitin oligosaccharide structure that is deacetylated, acylated, methylated, carbamoylated and sulfated by the products of nodB , nodA , nodS , nodU and nodH , respectively [49,50], and exported by an ABC-type transporter encoded by nodIJ [52]. The activated sulfate donor used by NodH is produced by the nodPQ products.…”

Section: Resultsmentioning

confidence: 99%

Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.)

et al. 2012

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BackgroundRhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 are α-Proteobacteria that establish nitrogen-fixing symbioses with a range of legume hosts. These strains are broadly used in commercial inoculants for application to common bean (Phaseolus vulgaris) in South America and Africa. Both strains display intrinsic resistance to several abiotic stressful conditions such as low soil pH and high temperatures, which are common in tropical environments, and to several antimicrobials, including pesticides. The genetic determinants of these interesting characteristics remain largely unknown.ResultsGenome sequencing revealed that CIAT 899 and PRF 81 share a highly-conserved symbiotic plasmid (pSym) that is present also in Rhizobium leucaenae CFN 299, a rhizobium displaying a similar host range. This pSym seems to have arisen by a co-integration event between two replicons. Remarkably, three distinct nodA genes were found in the pSym, a characteristic that may contribute to the broad host range of these rhizobia. Genes for biosynthesis and modulation of plant-hormone levels were also identified in the pSym. Analysis of genes involved in stress response showed that CIAT 899 and PRF 81 are well equipped to cope with low pH, high temperatures and also with oxidative and osmotic stresses. Interestingly, the genomes of CIAT 899 and PRF 81 had large numbers of genes encoding drug-efflux systems, which may explain their high resistance to antimicrobials. Genome analysis also revealed a wide array of traits that may allow these strains to be successful rhizosphere colonizers, including surface polysaccharides, uptake transporters and catabolic enzymes for nutrients, diverse iron-acquisition systems, cell wall-degrading enzymes, type I and IV pili, and novel T1SS and T5SS secreted adhesins.ConclusionsAvailability of the complete genome sequences of CIAT 899 and PRF 81 may be exploited in further efforts to understand the interaction of tropical rhizobia with common bean and other legume hosts.

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“…No function has, however, yet been described for the hsnT genes in these strains. The NFs of CIAT 899 have been analysed [37]– [39], but no acetyl substituent has been reported in the same position as in N. galegae . The acetyl substituent of N. galegae is in a very unusual position, on the GlcNAc residue adjacent to the non-reducing-terminal residue, while Nod factors of CIAT 899 are acetylated on the non-reducing-terminal residue.…”

Section: Discussionmentioning

confidence: 99%

Genome sequencing of two Neorhizobium galegae strains reveals a noeT gene responsible for the unusual acetylation of the nodulation factors

et al. 2014

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BackgroundThe species Neorhizobium galegae comprises two symbiovars that induce nodules on Galega plants. Strains of both symbiovars, orientalis and officinalis, induce nodules on the same plant species, but fix nitrogen only in their own host species. The mechanism behind this strict host specificity is not yet known. In this study, genome sequences of representatives of the two symbiovars were produced, providing new material for studying properties of N. galegae, with a special interest in genomic differences that may play a role in host specificity.ResultsThe genome sequences confirmed that the two representative strains are much alike at a whole-genome level. Analysis of orthologous genes showed that N. galegae has a higher number of orthologs shared with Rhizobium than with Agrobacterium. The symbiosis plasmid of strain HAMBI 1141 was shown to transfer by conjugation under optimal conditions. In addition, both sequenced strains have an acetyltransferase gene which was shown to modify the Nod factor on the residue adjacent to the non-reducing-terminal residue. The working hypothesis that this gene is of major importance in directing host specificity of N. galegae could not, however, be confirmed.ConclusionsStrains of N. galegae have many genes differentiating them from strains of Agrobacterium, Rhizobium and Sinorhizobium. However, the mechanism behind their ecological difference is not evident. Although the final determinant for the strict host specificity of N. galegae remains to be identified, the gene responsible for the species-specific acetylation of the Nod factors was identified in this study. We propose the name noeT for this gene to reflect its role in symbiosis.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-500) contains supplementary material, which is available to authorized users.

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Characterization ofRhizobium tropiciClAT899 Nodulation Factors: The Role ofnodHandnodPQGenes in Their Sulfation

Cited by 69 publications

References 0 publications

Distribution and Diversity of Rhizobial Populations Associated with <i>Acacia senegal</i> (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Distribution and Diversity of Rhizobial Populations Associated with <i>Acacia senegal</i> (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.)

Genome sequencing of two Neorhizobium galegae strains reveals a noeT gene responsible for the unusual acetylation of the nodulation factors

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Characterization ofRhizobium tropiciClAT899 Nodulation Factors: The Role ofnodHandnodPQGenes in Their Sulfation

Cited by 69 publications

References 0 publications

Distribution and Diversity of Rhizobial Populations Associated with &lt;i&gt;Acacia senegal&lt;/i&gt; (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Distribution and Diversity of Rhizobial Populations Associated with &lt;i&gt;Acacia senegal&lt;/i&gt; (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.)

Genome sequencing of two Neorhizobium galegae strains reveals a noeT gene responsible for the unusual acetylation of the nodulation factors

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Distribution and Diversity of Rhizobial Populations Associated with <i>Acacia senegal</i> (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions

Distribution and Diversity of Rhizobial Populations Associated with <i>Acacia senegal</i> (L.) Willd. Provenances in Senegalese Arid and Semiarid Regions