Characterization of Silybum marianum triacylglycerol regioisomers using accurate mass quadrupole time of flight mass spectrometry

Regiospecific Analysis of Triacylglycerols by Ultrahigh-Performance-Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry

“…Therefore, it is not possible to apply one common calibration curve for regiospecific quantification of different molecular species of TGs. Papers reporting regioisomeric analysis of TGs have rarely reported detailed calibration data, or have analyzed a very limited number of reference compounds. − Further, the more complex quantification of ABC type TGs is often overlooked, and no calibration curves have so far been reported for regiopure ABC type TGs that can be applied for ESI–MS/MS. ,− ,,,, …”

“…33−36 Further, the more complex quantification of ABC type TGs is often overlooked, and no calibration curves have so far been reported for regiopure ABC type TGs that can be applied for ESI−MS/MS. 19,[23][24][25]29,30,35,37 The aim of the current study was to develop a more accurate and robust ammonia adduct ESI−MS/MS method for routine quantification of regioisomeric composition of AAB and ABC type TGs that can be applied for studying natural fats and oils. To reach this goal, a large collection of reference compounds of regiospecific TGs were analyzed to obtain calibration curves for regiospecific quantification of these TGs.…”