Characterization of Staphylococcus aureus Isolated From Wound Infectious in Diabetes Clinic of Hazrat Fatemeh Zahra (SA) Hospital

Abstract

Background Staphylococcus aureus has long been recognized as an important pathogen of hospital acquired infections (1). Due to the expansion of the drug resistance pattern and importance of antibiotic resistance, the study of these microbial strains is one of the major challenges which should be taken into consideration (2). Methicillin resistance in S. aureus is mediated by a penicillin binding protein (PBP2a) which is encoded by the mecA gene (2-4). According to previous studies, 50% to 90% of S. aureus strains isolated from hospital infections were resistant to methicillin (5,6). Staphylococcus aureus is the most common cause of skin and soft-tissue infections like impetigo, furunculosis, superficial and surgical wounds and abscess (7-13). PIA is a polysaccharide composed of β1-6-linked N-acetyl glucosamine with partially deacetylate residues, which encloses the human cells or medical tools and protects the microorganism against both host immune system and antibiotic treatment (6). In this study, we investigated effective MSCRAMM-encoding genes, during biofilm formation on Congo Red Agar (CRA) medium and polystyrene plates, via phenotypic and genotypic screening of methicillin resistant S. aureus strains isolated from the patients with nosocomial infections in Hazrat Fatemeh Zahra (SA) hospital, Isfahan, Iran. Methods and Materials Bacterial Isolation and Culture Conditions Sample collection and Staphylococcus identification were performed in diabetes clinic of Hazrat Fatemeh Zahra (SA) hospital, Isfahan, Iran. A total of 267 clinical samples were selected from various types of wound infections, from which 132 samples were S. aureus. The isolates were selected from infectious wounds in patients hospitalized in diabetes clinic of Hazrat Fatemeh Zahra