Characterization of <i>Weissella ceti</i> infections in Brazilian rainbow trout, <i>Oncorhynchus mykiss</i> (Walbaum), farms and development of an oil‐adjuvanted vaccine

Costa, F A. A.; Leal, Carlos Augusto Gomes; Schuenker, N D; Leite, Rômulo Cerqueira; Figueiredo, Henrique César Pereira

doi:10.1111/jfd.12236

Cited by 18 publications

(14 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…; Costa et al . ) and the United States (Welch and Good ). Similar disease signs and high rates of mortality were associated with each outbreak, indicating that substantial economic losses are possible if disease is left untreated and prevalence increases.…”

Section: Introductionmentioning

confidence: 99%

“…Weissellosis is a rapidly emerging disease of intensively farmed rainbow trout, with recent outbreaks in China (Liu et al 2009), Brazil (Figueiredo et al 2012;Costa et al 2014) and the United States (Welch and Good 2013). Similar disease signs and high rates of mortality were associated with each outbreak, indicating that substantial economic losses are possible if disease is left untreated and prevalence increases.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Diagnostic tools for rapid detection and quantification of Weissella ceti NC36 infections in rainbow trout

Snyder

Hinshaw

Welch

2015

Letters in Applied Microbiology

View full text Add to dashboard Cite

Weissellosis of rainbow trout is caused by the Gram-positive bacteria Weissella ceti and has been reported in China, Brazil and the United States. This disease can result in high mortality in market-sized fish and thus can cause significant economic loss. Thus far, phenotypic characterization and 16S rRNA sequencing have been used to confirm a Weissellosis diagnosis. Here, we present the development of PCR-based diagnostic tools for the rapid identification and quantification of W. ceti within bacteriological culture and infected tissues. A duplex PCR, which amplifies both genus- and strain-specific targets, positively identifies isolates as W. ceti NC36. A qPCR assay was also developed to quantify pathogen load from infected tissues, using a W. ceti NC36 unique locus. A proof of concept study was performed to demonstrate that quantification using traditional plate count methods and qPCR were significantly correlated when assessed from infected brain and spleen tissue. These tools were also used to confirm diagnosis of Weissellosis in a commercial rainbow trout farm during an outbreak investigation. These are the first diagnostic tools developed for identification and quantification of W. ceti infection within rainbow trout, contributing to rapid Weissellosis diagnosis, enhanced pathogen surveillance and epidemiological studies.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diagnostic tools for rapid detection and quantification of Weissella ceti NC36 infections in rainbow trout

Snyder

Hinshaw

Welch

2015

Letters in Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…Actually, four Weissella species have been associated with health disorders and infections, both in animals or humans: W. cibaria, W. confusa, W. viridescens and W. ceti. W. ceti is considered to be a pathogen which infects fish and rainbow trout [193,194]. Genome sequence analysis of W. ceti strains revealed the presence of several virulence factors and antibiotic resistance genes [195], which comforts its potential pathogenicity.…”

Section: Infections Associated With Weissellamentioning

confidence: 99%

Why Are Weissella spp. Not Used as Commercial Starter Cultures for Food Fermentation?

2017

View full text Add to dashboard Cite

Among other fermentation processes, lactic acid fermentation is a valuable process which enhances the safety, nutritional and sensory properties of food. The use of starters is recommended compared to spontaneous fermentation, from a safety point of view but also to ensure a better control of product functional and sensory properties. Starters are used for dairy products, sourdough, wine, meat, sauerkraut and homemade foods and beverages from dairy or vegetal origin. Among lactic acid bacteria, Lactobacillus, Lactococcus, Leuconostoc, Streptococcus and Pediococcus are the majors genera used as starters whereas Weissella is not. Weissella spp. are frequently isolated from spontaneous fermented foods and participate to the characteristics of the fermented product. They possess a large set of functional and technological properties, which can enhance safety, nutritional and sensory characteristics of food. Particularly, Weissella cibaria and Weissella confusa have been described as high producers of exo-polysaccharides, which exhibit texturizing properties. Numerous bacteriocins have been purified from Weissella hellenica strains and may be used as bio-preservative. Some Weissella strains are able to decarboxylate polymeric phenolic compounds resulting in a better bioavailability. Other Weissella strains showed resistance to low pH and bile salts and were isolated from healthy human feces, suggesting their potential as probiotics. Despite all these features, the use of Weissella spp. as commercial starters remained non-investigated. Potential biogenic amine production, antibiotic resistance pattern or infection hazard partly explains this neglecting. Besides, Weissella spp. are not recognized as GRAS (Generally Recognized As Safe). However, Weissella spp. are potential powerful starters for food fermentation as well as Lactococcus, Leuconostoc or Lactobacillus species.

show abstract

“…Despite the number of isolates being a limitation, particularly since both were obtained from the same farm during a single sampling, the genotyping results suggest that these Mexican isolates would represent the same strain of W. ceti . Interestingly, Costa, Leal, Schuenker, Leite, and Figueiredo () analysed 34 Brazilian isolates recovered from different outbreaks in different years using REP‐PCR, ERIC‐PCR and PFGE, showing that the W. ceti isolates associated with disease outbreaks in Brazilian farms are clonally related. A comparative analysis between Brazilian W. ceti strains and a USA isolate also demonstrated a high degree of similarity despite the lack of epidemiological linkage between farms and between countries (Figueiredo et al., ).…”

Section: Resultsmentioning

confidence: 99%

Isolation characterization, virulence potential ofWeissella cetiresponsible for weissellosis outbreak in rainbow trout (Oncorhynchus mykiss) cultured in Mexico

Castrejón-Nájera

Ortega

Fajardo

et al. 2018

Transbound Emerg Dis

View full text Add to dashboard Cite

Weissella ceti, a Gram-positive nonmotile bacterium, is currently an emerging pathogen within rainbow trout (Oncorhynchus mykiss) farms in China, Brazil, the United States, and Japan. This study is the first to isolate, identify, and characterize W. ceti isolates from rainbow trout farmed in Mexico. In late 2015, a severe disease outbreak caused a 60% mortality rate among 20,000 fish. The diseased rainbow trout (100-300 g average) exhibited severe cachexia, body darkening, abdominal distension, exophthalmia, haemorrhages, and corneal opacity. Internally, diseased fish had pale gills; multifocal, disseminated whitish spots on the liver; haemorrhages in the swim bladder, ovary, and on the parietal surface of the muscle; and hearts with pseudo-membrane formation. Histologically, lesions were characterized by corneal oedema, degenerative and necrotic hepatitis, and meningitis. A brain (W-1) and kidney (W-2) isolate were identified as W. ceti through polyphasic taxonomy, which included phenotypic characterization and 16S rRNA sequencing. RAPD and ERIC-PCR analyses demonstrated genetic homogeneity among the Mexican isolates. Virulence tests in rainbow trout through intraperitoneal W. ceti injections at concentrations of 1 × 10 , 1 × 10 , and 1 × 10 CFU per fish resulted in cumulative mortality rates of 25%, 62.5%, and 87.5%, respectively, as well as the same clinical signs of hemorrhagic septicaemia as were recorded for the natural outbreak. The present report is the first to confirm the presence of W. ceti in Mexico, thus extending the known geographical distribution of this pathogen across the Americas.

show abstract

Characterization of Weissella ceti infections in Brazilian rainbow trout, Oncorhynchus mykiss (Walbaum), farms and development of an oil‐adjuvanted vaccine

Cited by 18 publications

References 23 publications

Diagnostic tools for rapid detection and quantification of Weissella ceti NC36 infections in rainbow trout

Diagnostic tools for rapid detection and quantification of Weissella ceti NC36 infections in rainbow trout

Why Are Weissella spp. Not Used as Commercial Starter Cultures for Food Fermentation?

Isolation characterization, virulence potential ofWeissella cetiresponsible for weissellosis outbreak in rainbow trout (Oncorhynchus mykiss) cultured in Mexico

Contact Info

Product

Resources

About