Characterization of imidacloprid resistance in Aphis gossypii (Glover) (Hemiptera: Aphididae) in Southern Iran

Seyedebrahimi, S.; Talebi, Khalil; Imani, Sohrab; Naveh, Vahid Hosseini; Hesami, Shahram

doi:10.16970/ted.67424

Cited by 6 publications

(6 citation statements)

References 34 publications

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“…The P450 activities also parallel the bioassay results. Some studies have reported that the resistant aphid populations have higher P450 activity compared to susceptible aphid populations (Shang et al, 2012;Seyedebrahimi et al, 2015). According to some studies, the neonicotinoid resistance mechanism is associated with mutations in the nAChR gene, but is usually directly associated with xenobiotic detoxification enzyme, 7-ethoxycoumarin O-deethylase, which is catalyzed by cytochrome P450 (Karunker et al, 2008;Nauen et al, 2008;Wang et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In insects, cytochrome P450 monooxygenases (P450) is implicated in resistance to insecticides through the degradation of these foreign compounds to more soluble and less toxic forms (Scott & Wen, 2001;Rauch & Nauen, 2003). In addition, high levels of P450 enzyme activity were found in neonicotinoid insecticide resistant A. gossypii populations (Shang et al, 2012;Seyedebrahimi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Neonicotinoid resistance of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) in cotton fields of Çukurova Region, Turkey

Ulusoy

Atakan

Dincer

2018

Turkish Journal of Entomology

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Cotton aphid, Aphis gossypii Glover, 1877 (Hemiptera: Aphididae), is a major pest in cotton fields. Neonicotinoids are important and highly prevalent insecticides currently used against A. gossypii and other herbivorous insect pests in the Mediterranean Region of Turkey. However, some insecticide applications against A. gossypii in the Çukurova Region have failed despite using high rates. Therefore, bioassays and enzyme analyses were conducted to determine resistance to imidacloprid and thiamethoxam in A. gossypii populations collected in 2015-2016 from cotton fields in this region. Resistance factors (RF) were 54.6 to 206.5 fold for imidacloprid and 5.7 to 65.7 fold for thiamethoxam. Populations from Kürkçüler (RF 206.5) had the highest LD50 for imidacloprid and from Körkuyu (RF 65.7) for thiamethoxam. Enzyme analysis revealed statistically higher metabolic resistance. Maximum enzyme activities were 17.8, 142.3 and 3.8 nM/min/mg protein for carboxylesterase for in Körkuyu, for glutathione S-transferase in Bahçe and for cytochrome P450 monooxygenase in Körkuyu, respectively. This study revealed the development of resistance in A.gossypii to neonicotinoid insecticides in Turkey and the need for new management strategies to break this resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Neonicotinoid resistance of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) in cotton fields of Çukurova Region, Turkey

Ulusoy

Atakan

Dincer

2018

Turkish Journal of Entomology

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“…The activity of GST was determined using the method described in [ 21 ]. The activity of Cyt-P450s was measured using the 7-ethoxycoumarin-O-demethylase protocol was described in [ 22 , 23 ]. Protein and enzymes activity were measured in the combinations that gave the highest rate of potentiation or an antagonism, where they were at the ratio of 1:1 only.…”

Section: Methodsmentioning

confidence: 99%

The Binary Mixtures of Lambda-Cyhalothrin, Chlorfenapyr, and Abamectin, against the House Fly Larvae, Musca domestica L.

et al. 2022

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The house fly Musca domestica L. is one of the medical and veterinary pests that can develop resistance to different insecticides. Mixing insecticides is a new strategy for accelerating pest control; furthermore, it can overcome insect resistance to insecticides. This study aims to evaluate three insecticides, chlorfenapyr, abamectin, and lambda-cyhalothrin, individually and their binary mixtures against 2nd instar larvae of M. domestica laboratory strain. Chlorfenapyr exhibited the most toxic effect on larvae, followed by abamectin then the lambda-cyhalothrin. The half-lethal concentrations (LC50) values were 3.65, 30.6, and 94.89 ppm, respectively. These results revealed that the high potentiation effect was the mixture of abamectin/chlorfenapyr in all the mixing ratios. In contrast, the tested combination of lambda-cyhalothrin/abamectin showed an antagonism effect at all mixing ratios against house fly larvae. The total protein, esterases, glutathione-S-transferase (GST), and cytochrome P-450 activity were also measured in the current investigation in the larvae treated with chlorfenapyr. Our results indicate that GST may play a role in detoxifying chlorfenapyr in M. domestica larvae. The highest activity of glutathione-S-transferase was achieved in treated larvae with chlorfenapyr, and an increase in cytochrome P-450 activity in the larvae was observed post-treatment with Abamectin/chlorfenapyr.

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“…Cytochrome P450 monooxygenases are a broad family of enzymes that have functions of metabolic activities such as hormone synthesis in the insect's body and making xenobiotics less toxic or water-soluble in their detoxification (Rauch andNauen 2003, Scott andWen 2001). High P450 enzyme activity levels were reported in neonicotinoid-resistant A. gossypii populations (Seyedebrahimi et al 2015, Shang et al 2012, Ulusoy et al 2018. It was stated that this situation is dependent on the changes in the expression levels of P450 genes, changes in catalytic activity or changes in substrate-specificity (Bergé et al 1998, Hirata et al 2017, Nikou et al 2003, Wu et al 2018.…”

mentioning

confidence: 99%

Overexpression of cytochrome P450 genes in Aphis gossypii (Glover) in the cotton fields of the Çukurova region, Turkey

Ulusoy¹

2020

Bitki Koruma Bülteni

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Aphis gossypii Glover, 1877 (Hemiptera: Aphididae), which is a polyphagous species, is among the main pests of the cotton plants in the whole world. The Çukurova region is an area where polyculture agriculture activities are carried out. While insecticides in the neonicotinoid group are prevalently used in the cotton fields in this region for controlling A. gossypii, the problem of resistance is frequently encountered. This study determined the relative expression levels of the CYP6CY22 and CYP6CY13 genes, cytochrome P450, acetylcholine esterase (AChE) and glutathione S-transferase (GST) enzyme activities in six different populations of A. gossypii collected in 2018 from cotton fields in the province of Adana in Southern Turkey. In comparison to the reference culture, there was an increase in gene regulation by 81.9 fold in the CYP6CY22 gene in the Hamitbey population and by 6 fold in the CYP6CY13 gene in the Gazipaşa population. Additionally, the Hamitbey population showed 4.3 times higher (0.74 U/ml) cytochrome P450 enzyme activity. The Çiftlikler population showed 5.9 fold higher AChE (0.82 U/ml) and 4.3 fold higher GST (2.68 U/ml) activities. The analyses revealed that the A. gossypii populations were exposed to high amounts of insecticides. Consequently, overexpressed genes, high levels of enzyme activities and metabolic resistance in based on cytochrome P450 activity were observed in the study. Cotton aphid, Aphis gossypii Glover, 1877 (Hemiptera: Aphididae), is one of the significant pests among aphids that reaches the economic loss threshold. A. gossypii is a polyphagous species that has a very broad spectrum of hosts. As in the entire world, they create harm on especially cotton and Cucurbitaceae species in Turkey (Ozgur and Sekeroglu

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Characterization of imidacloprid resistance in Aphis gossypii (Glover) (Hemiptera: Aphididae) in Southern Iran

Cited by 6 publications

References 34 publications

Neonicotinoid resistance of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) in cotton fields of Çukurova Region, Turkey

Neonicotinoid resistance of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) in cotton fields of Çukurova Region, Turkey

The Binary Mixtures of Lambda-Cyhalothrin, Chlorfenapyr, and Abamectin, against the House Fly Larvae, Musca domestica L.

Overexpression of cytochrome P450 genes in Aphis gossypii (Glover) in the cotton fields of the Çukurova region, Turkey

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