Characterization of immunoglobulin E responses in Balb/c mice against the major allergens of timothy grass (<i>Phleum pratense</i>) pollen

Seitzer, Ulrike; Bussler, Holm; Kullmann, Birgit; Petersen, Arnd; Becker, Wolf‐Meinhard; Ahmed, Jabbar S.

doi:10.1046/j.1365-2222.2003.01630.x

Cited by 11 publications

(8 citation statements)

References 28 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sensitization was carried out by intraperitoneal injections of 5 µg recombinant Phleum pratense major allergen Phl p5 (Biomay, Wien, Austria) adsorbed to 1 mg Alum Imject (Pierce, Rockford, USA) on days 0, 14, and 21. Mice were finally challenged on days 28 and 29 intranasally with 9 µg Phleum pratense extract (Alk-Scherax, Wedel, Germany) in 50 µL PBS as described elsewhere [19].…”

Section: T-lymphocyte Proliferation Assaymentioning

confidence: 99%

Surfactant protein D modulates pulmonary clearance of pollen starch granules

Winkler

Hüper

Wedekind

et al. 2010

Experimental Lung Research

View full text Add to dashboard Cite

Pollen starch granules (PSGs) are allergen particles that get into contact with pulmonary surfactant and phagocytes in the terminal airways. In this study, the effects of surfactant protein D (SP-D) on the interaction of PSGs with phagocytes and on the pulmonary clearance of PSGs were determined. Fluorescently labeled PSGs were incubated in vitro with murine lung macrophages or dendritic cells (DCs) ± recombinant rat SP-D (rrSP-D). In addition, the effect of SP-D on uptake of PSGs by lung macrophages and DCs was studied in vivo. Furthermore, PSGs were instilled in Balb/c mice and the effects of SP-D on total lung clearance were assessed by optical imaging. SP-D treatment increased the number of PSG-positive macrophages and DCs in vitro. Furthermore, SP-D accelerated uptake/binding by alveolar macrophages and reduced the number of PSG-positive tissue macrophages and DCs at 24 hours. However, SP-D did not affect total lung clearance of PSGs and it did not enhance the T-cell proliferation induced by PSG-positive DCs. In conclusion, SP-D increased PSG-positive cells in vitro and accelerated PSG binding/uptake in vivo. The observed effects were limited to cellular clearance mechanisms and did not affect the total clearance of PSGs from the lung.

show abstract

Section: T-lymphocyte Proliferation Assaymentioning

confidence: 99%

Surfactant protein D modulates pulmonary clearance of pollen starch granules

Winkler

Hüper

Wedekind

et al. 2010

Experimental Lung Research

View full text Add to dashboard Cite

show abstract

“…Strips incubated with the secondary antibodies alone served as negative controls. The major allergens which elicit an IgE response in BALB/c mice have been determined previously [9]. …”

Section: Methodsmentioning

confidence: 99%

“…Recently, we have described the IgE response of BALB/c mice to P. pratense pollen extract (PE) [9]and demonstrated that the IgE response to P. pratense allergens strongly resembles the one found in human patients, differing only in the lack of an IgE reactivity against Phl p 1 [9, 10]. To our knowledge, there is no study dealing with grass pollen allergens in association with the induction of IgE and ICHR to these clinically relevant allergens in different mouse strains.…”

Section: Introductionmentioning

confidence: 99%

Mouse Strain Specificity of the IgE Response to the Major Allergens of <i>Phleum pratense</i>

Seitzer¹,

Bussler

Kullmann³

et al. 2005

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: IgE immune responses against major allergens from Phleum pratense in low- and high-responder mouse strains were compared and the influence of alum was assessed, in order to evaluate the effect of the genetic background and adjuvants on IgE reactivity in a mouse model for P. pratense allergy. Methods: Different mouse strains and F1 offspring were sensitized with P. pratense pollen extract. Serum IgE levels, the induction of specific IgE antibodies and immediate cutaneous hypersensitivity reactions were monitored by ELISA, Western blot and a skin test, respectively. Results: All mouse strains investigated mounted an IgE response and exhibited a positive skin test to pollen extract. Differences were seen in the level of total serum IgE and in specific IgE reactivity to different major allergens of P. pratense. Notable differences were seen in IgE reactivity and immediate hypersensitivity against Phl p 1, which were only observed in SJL/j mice. The foremost influence of alum was on total IgE production levels. Conclusions: Alum is not necessary as an adjuvant to elicit IgE reactivity against the clinically relevant allergens of P. pratense, since even low-responder mouse strains mounted a hypersensitivity reaction after sensitization without the adjuvant using otherwise identical sensitization strategies. Moreover, when analyzing the allergenicity of a compound, the hypersensitivity response of different mouse strains should be considered, as implicated by the differential results obtained for IgE reactivity against Phl p 1. Lastly, a genetic component may be involved in IgE reactivity to Phl p 1.

show abstract

“…Considering that the dispersion of pine pollens is most intense for about 2 weeks, IgE induction experiments were conducted on mice for 15 days to induce allergic reactions via inhalation and skin application (Tamura 1986;Conford et al 1990;Maejima et al 2000;Mahler et al 2000;Seitzer et al 2003a, b;Mondoulet et al 2010Mondoulet et al , 2012Tordesillas et al 2015). Control mice showed no change in the IgE compositions of blood sera collected on days 5, 10, and 15 (Fig.…”

Section: Comparison Of Ige Induction By Pine Pollensmentioning

confidence: 99%

Comparison of IgE induction in mice by pollens from three pine tree species

Kim

Choi

2017

j ecology environ

View full text Add to dashboard Cite

Background: Over the years, pine pollens have been excluded as an allergen due to its relatively large size, low protein content, and waxy hydrophobic layer, despite their abundance. However, recent studies suggest the possibilities of pine pollens being allergens, and it has been reported that allergy symptoms were highly prevalent in areas with considerably large pine forests and high possibility of exposure to the pollen. Therefore, we conducted a comparative analysis of the allergenicities of the pollens from the dominant species of Korean pines, red pine (Pinus densiflora), black pine (Pinus thunbergii), and pitch pine (Pinus rigida), in mice. Methods: The protein composition of the pollens from the three pine species was compared via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The pine pollens and proteins extracted from the pollens were introduced to BALB/c mice by nasal inhalation and application to exposed skin and the IgE produced by the mice were extracted from blood and analyzed via ELISA. Results: SDS-PAGE showed differing protein compositions of the pollens of the three pine species. Analysis of blood IgE compositions showed a similar amount of IgE produced when pollens were applied to skin. In contrast, when mice inhaled the pollens, P. densiflora was shown to induce significantly more IgE production than those of the other two species. Conclusions: The experimental results demonstrate that the pollens of all three South Korean pine species induce IgE production, and this production was more pronounced when the pollens were inhaled than when they were applied to the skin. Of the three species, the pollen of P. densiflora was found to induce the highest level of IgE production.

show abstract

Characterization of immunoglobulin E responses in Balb/c mice against the major allergens of timothy grass (Phleum pratense) pollen

Cited by 11 publications

References 28 publications

Surfactant protein D modulates pulmonary clearance of pollen starch granules

Surfactant protein D modulates pulmonary clearance of pollen starch granules

Mouse Strain Specificity of the IgE Response to the Major Allergens of <i>Phleum pratense</i>

Comparison of IgE induction in mice by pollens from three pine tree species

Contact Info

Product

Resources

About