Characterization of immunophenotypic aberrancies in adult and childhood acute lymphoblastic leukemia: A study from Northern India

Sharma, Manupriya; Sachdeva, Man Updesh Singh; Varma, Neelam; Varma, Subhash; Marwaha, R. K.

doi:10.4103/0973-1482.147716

Cited by 22 publications

(28 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HLA-DR frequency in B-ALL was (91.6%) and for T-ALL was (12.5%). These results matched with Sharma et al, who found that CD34 expression in ALL were seen in 67.3% of all ALL cases and the expression was more frequent in B-ALL (70.3%) as compared to T-ALL (51%) [16]. Another Indian study found that CD34 positivity was much higher in B-lineage ALL (82%) than in T-ALL (35%), (p < 0.0001) [17].…”

Section: Discussionsupporting

confidence: 86%

“…In this study, the aberrancy percentage was 45.8% of all leukemic patients, was found in 66 patients, lymphoid antigen markers expression in AML was in 40 patients (45.5%), and aberrancy incidence of myeloid antigen expression on ALL was in 26 patients (46.4%). Some studies showed comparable results, in which aberrant myeloid antigen expression was seen in 42.5% of ALL cases and 47% of AML cases showed aberrant lymphoid antigen expression [16] [20]. On the other hand many studies showed lower frequency of aberrant immunophenotyping [7] [21].…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Flow Cytometric Diagnosis of Acute Leukemia and Aberrant Antigen: Sohag University Experience

Hamed¹,

El-Deen²

2018

OJBD

View full text Add to dashboard Cite

Background: Multiparameter flow cytometry is the most important method for the lineage assignment and maturational analysis of acute leukemias (AL) cells. The multi parametric immunophenotyping analysis allows the detection of aberrant antigen expression and the analysis of heterogeneity and clonality of malignant cells in AL. The aim of the work is to study the immunophenotypes of blasts from patients with AL and determine the frequency of aberrant markers. Subjects and Methods: Retrospective study to analyze immunophenotypic data of de novo 144 AL patients who were diagnosed in Clinical Pathology Department, Sohag University. Results: We found that 61.8% of AL patients were classified as acute myeloblastic leukemia (AML) while 38.9% classified as acute lymphoblastic leukemia (ALL). The commonest FAB subtype in AML group was AML-M2 (31.8%) followed by M4-M5 27.3%. As regard ALL, there were 85.7% with B-ALL and 14.3% with T-ALL. The aberrancy expressions were found in 66 of AL cases (45.8%), CD7 was the most commonly expressed lymphoid antigen in AML (25%), CD13 was the most commonly expressed myeloid antigen in ALL (39.3%). Conclusion: The multi parametric immunophenotyping analysis of AL is sufficient for diagnosis and classification of leukemia. The frequencies of aberrant markers in AL were matched with many published data.

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 98%

Flow Cytometric Diagnosis of Acute Leukemia and Aberrant Antigen: Sohag University Experience

Hamed¹,

El-Deen²

2018

OJBD

View full text Add to dashboard Cite

show abstract

“…Contudo, a expressão do antígeno mielóide é maior em casos de LLA em adultos do que em crianças. (Sharma et al, 2016) A adição dos marcadores CD135 e CD117 ao diagnóstico pode prever aberrações moleculares em configurações de T-ALL em crianças, principalmente segregando pacientes com FLT3-ITD, que se beneficiariam do tratamento com inibidores de tirosina. (Noronha et al, 2016) Para Ren et al (2019) a combinação dos anticorpos CD9, CD11b e HLA-DR apresenta alta sensibilidade e especificidade e pode ser utilizada para diagnosticar APL.…”

Section: Discussõesunclassified

Análise de métodos imunofenotípicos no diagnóstico precoce de leucemias agudas

Santos¹,

Leite²,

Abreu³

et al. 2020

RSD

View full text Add to dashboard Cite

Objetivo: Evidenciar a importância da utilização da imunofenotipagem no diagnóstico precoce de leucemias agudas. Metodologia: Trata-se de uma revisão integrativa da literatura, onde foram utilizados 12 artigos publicados entre 2016 e 2020. O idioma dos artigos referenciados foram inglês e francês, coletados no Scielo, LILACS, BVS e EBSCO. Utilizando a busca avançada para os seguintes descritores: Immunophenotyping, Acute Leukemia, Flow cytometry. Resultado: Os artigos utilizados foram publicados entre os anos de 2016 e 2020. Os artigos explicitaram que o uso da imunofenotipagem, especificamente a citometria de fluxo, é um método de extrema importância para o diagnóstico de leucemias agudas. Foram apontados marcadores utilizados em cada tipo e subtipo de leucemia, evidenciando assim a peculiaridade da doença. Conclusão: A utilização de métodos imunofenotípicos com exames de rotina contribui no diagnóstico precoce de pacientes com leucemias agudas, fazendo com que o tratamento seja feito também de forma precoce, aumentando assim a sobrevida desses indivíduos. De acordo com os estudos analisados, a imunofenotipagem está em constante evolução e promete simplificar o processo de diagnóstico de vários tipos de leucemias.

show abstract

“…5 on neoplastic cells different from the process of normal hematopoietic maturation. 7 In ALL, these aberrancies include: a) Expression of myeloid antigens in ALL, B-lineage antigens in T-ALL or T-lineage antigens in B-ALL, b) co-expression of early (immaturity associated) antigens with mature ones c) antigen over expression or under expression or absence of expression. 7 The expression of aberrant myeloid antigen (MyAg) has varied between 5 and 86% in various studies.…”

Section: Introductionmentioning

confidence: 99%

“…7 In ALL, these aberrancies include: a) Expression of myeloid antigens in ALL, B-lineage antigens in T-ALL or T-lineage antigens in B-ALL, b) co-expression of early (immaturity associated) antigens with mature ones c) antigen over expression or under expression or absence of expression. 7 The expression of aberrant myeloid antigen (MyAg) has varied between 5 and 86% in various studies. 7 The expression of two or more lineage specific antigens poses a dilemma in assigning leukemia to any one lineage.…”

Section: Introductionmentioning

confidence: 99%

Aberrant immunophenotypic expressions in acute lymphoid leukemia: an observational analytical study

Sivakumar

Basu

2021

Int J Res Med Sci

View full text Add to dashboard Cite

Background: This study aims to find out the expression of aberrant immunophenotypic markers in acute lymphoid leukemia (ALL) and to co-relate its expression with cytogenetic and molecular data.Methods: Retrospective cum prospective study was carried out in 75 patients of ALL who presented to Apollo Gleneagles hospitals, Kolkata from January 2014 and March 2019. Flow cytometry analysis was done using FC500 (Beckman coulter) All cases were classified according to latest WHO classification.Results: Out of 75 cases of ALL, 23 cases (30.67%) showed aberrant cross-lineage expression. Amongst the B-ALL cases, the most common aberrant antigen expressed were myeloid antigens 17 cases (77.27%). Aberrant T- antigens were noted in 4 cases (18.10%). Aberrant co-positivity of myeloid as well as T-antigens was seen in 1 case (4.54%). The most common aberrant myeloid antigen expressed was CD33 (77.7%) followed by CD13 (22.2%) and then CD15 (11.1%). Co-positivity of CD13 and CD33 was noted in 2 cases. CD2 and CD33 co-positivity was noted in 1 case. The most frequently expressed aberrant T-antigen was CD2 seen in 3 out of 5 cases (60%).Conclusions: In B-ALL, the most common aberration was myeloid antigen positivity followed by cross-lineage T-antigen expression. Aberrant CD33 expression was most frequently associated with t(9;22) followed by t(12;21). Aberrant CD15 was most frequently associated with t(4;11). No association with adverse hematological parameters or any significant increase in cytogenetic and molecular abnormality was noted in cases expressing aberrant antigen in comparison to cases not expressing aberrant antigens.

show abstract

Characterization of immunophenotypic aberrancies in adult and childhood acute lymphoblastic leukemia: A study from Northern India

Cited by 22 publications

References 0 publications

Flow Cytometric Diagnosis of Acute Leukemia and Aberrant Antigen: Sohag University Experience

Flow Cytometric Diagnosis of Acute Leukemia and Aberrant Antigen: Sohag University Experience

Análise de métodos imunofenotípicos no diagnóstico precoce de leucemias agudas

Aberrant immunophenotypic expressions in acute lymphoid leukemia: an observational analytical study

Contact Info

Product

Resources

About