Characterization of Indonesia Decellularized Liver Cubes Scaffold using Scanning Electron Microscopy

Abstract

Liver biological scaffold was developed in order to resemble native liver tissue environment. It can be achieved by decellularizing native liver tissue that will remove cells and preserve extracellular matrix (ECM). Furthermore, ECM fibers are arranged in a special pattern, which affect liver cell polarity and topography that are important for cells’ implantation, proliferation and differentiation. Therefore, the aim of this study was to evaluate liver cube scaffold topography that was decellularized with fixed multiple sites syringe injection (Indonesia patent number: S00201907930).Rat liver cubes (n=3) underwent decellularization with Ethylene Glycol Tetraacetic Acid (EGTA) immersion and increased Sodium Dodecyl Sulfate (SDS) concentrations using previous multiple sites syringe injection protocol study. Deoxyribonucleic Acid (DNA) concentrations were measured to confirm less DNA materials remaining in scaffolds. Scanning Electron Microscope (SEM) analysis of scaffolds were conducted for topographic characterization compared to undecellularized liver control. Molecular analysis of DNA concentration showed complete removal of DNA material. SEM analysis gave appearance of intact liver cube scaffold microarchitecture. Liver cubes decellularization using multiple sites syringe injection showed good topographic liver scaffold characterization.

Hepatocyte Differentiation from iPSCs or MSCs in Decellularized Liver Scaffold: Cell–ECM Adhesion, Spatial Distribution, and Hepatocyte Maturation Profile

“…A simple method to decellularize liver scaffold has been established and characterized by the Department of Histology, Faculty of Medicine, Universitas Indonesia, in SCTE IMERI lab. 14 , 15 This study aims to compare the profile of hepatocyte differentiation from iPSCs or MSCs in decellularized liver scaffold. The research was conducted from January 2020 to June 2021 at the Stem Cell and Tissue Engineering (SCTE) laboratory of IMERI FKUI, Histology laboratory, Center laboratory for Materials and Processing Failure Analysis (CMPFA) Metallurgical Engineering FTUI, Molecular Laboratory Biology and Proteomic Core Facilities (MBPCF) IMERI FKUI, and the Human Genetic Research Center (HGRC) laboratory of IMERI FKUI.…”