Characterization of Indonesian Tangerine Cultivar by Morphological and Issr Markers

Martasari, Chaireni; Agisimanto, Dita; Karsinah,; Reflinur,

doi:10.17660/actahortic.2013.975.10

Cited by 8 publications

(6 citation statements)

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“…A total of 14 ISSR primers previously evaluated by other researchers (Shahsavar et al 2007, Uzun et al 2010, Martasari et al 2012, Golein et al 2013, Tripolitsiotis et al 2013 were used (table 2). DNA concentration was measured by NanoDrop 1000 at 260 nm.…”

Section: Dna Extraction and Issr Analysismentioning

confidence: 99%

Genetic Diversity in Persian Lime (Citrus Latifolia Tanaka) Accessions Using Morphological and Molecular Markers

Khankahdani¹,

Rastegar²,

Golein³

et al. 2017

AgricultForest

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Section: Dna Extraction and Issr Analysismentioning

confidence: 99%

Genetic Diversity in Persian Lime (Citrus Latifolia Tanaka) Accessions Using Morphological and Molecular Markers

Khankahdani¹,

Rastegar²,

Golein³

et al. 2017

AgricultForest

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“…Moreover, Shrestha et al (2012) demonstrated the role of morphological characters in distinguishing five landraces of Citrus aurantifolia and subsequently used those characters as the main basis in genotype selection for breeding programs. Morphological characterization on 20 cultivars of Indonesian Citrus nobilis by Martasari and Reflinur (2012) highlighted contribution of morphology in recognizing major differences among cultivars.…”

Section: Discussionmentioning

confidence: 99%

ASSESSMENT OF TAXONOMIC AFFINITY OF INDONESIAN PUMMELO (<i>CITRUS MAXIMA</i> (BURM.) MERR.) BASED ON MORPHOLOGICAL CHARACTERS

Susandarini

Subandiyah

Rugayah³

et al. 2013

American Journal of Agricultural and Biological Sciences

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Citrus maxima is a tropical fruit species with high morphological variability on fruit characters, including shape, size, thickness of peel, flesh color and taste. This variability has been used by breeders and common people to practically distinguish different pummelo cultivars. Despite such well known variation, so far there is no published study specifically addressed the extent of phenotypic variability and taxonomy of pummelo in Indonesia. This study was, therefore, aiming at assessing phenotypic variability and taxonomic affinity of Indonesian pummelo cultivars and landraces using multivariate analysis methods. A total of 60 accessions from six provinces were collected, including some representatives of registered cultivars. Twenty one morphological characters were subjected to cluster analysis and principal component analysis. Results showed that two major clusters were formed, in which each group was characterized by combination of distinctive morphological features leading to recognition of two cultivar groups. Based on the fact that the morphology of individuals from intensively cultivated orchards showed similar level of variability to those from sporadically distributed areas, there was strong indication that the morphological variation has genetic background.

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“…PCR was conducted at the initial denaturation at 95 o C for 60 seconds, annealing with temperature according to the primers for 45 seconds, and extension at 72 o C for 60 seconds, for 30 cycles. The PCR process ended with a final extension at 72 o C for 5 minutes (Martasari, 2012). PCR product was then separated with 1% agarose gel by electrophoresis technique.…”

Section: Dna Extraction and Issr Analysismentioning

confidence: 99%

The Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions

et al. 2019

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Abstract. Saputro TB, Muslihatin W, Wahyuni DK, Nurhidayati T, Wardhani FO, Rosalia E. 2019. Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions. Biodiversitas 20: 3299-3308. Soybean (Glycine max L.) is the main food commodity that contains high proteins, oils, and carbohydrates. Waterlogging is considered as the dominant factor and major constraint responsible for the decrements of soybean production in Indonesia. The development of promising G.max that tolerates waterlogging is essentially needed. In this study, induction of variation was conducted by gamma rays irradiation with doses of 25Gy, 50Gy, 75Gy, and 100Gy. The mutant or variants lines were then selected under waterlogging conditions with 100%, 150%, 200%, and 250% of field capacity. The results showed that, in 250% waterlogging condition, plants irradiated with 25Gy shows the best performance in amount of roots, adventitious roots and number of pods parameters, while plants irradiated with 50Gy have the highest growth indicated by amount of root nodules, plant heights, dry weights, leaf area, and chlorophyll contents. The genotypes then were assayed by the Inter Simple Sequence Repeats (ISSR) to confirm that the mutants differ compared to its wild type. Out of 10 ISSR primers, seven primers showed polymorphic patterns. The best primer to differentiate the mutant lines and its wild type is primer ISSR1 [ (AC)8G] that able to generate the highest level of polymorphism with 44.0%. The comparison of protein profiles among the mutant lines showed that proteins with molecular weight 53,78 KDa; 43,12 KDa; and 20,62 Kda are all overexpressed for plants irradiated at 25Gy and treated with 250% waterlogging stress. All those three proteins are predicted as 1-amino cyclopropane-1-carboxylate synthase (ACS), Alcohol-dehydrogenase (ADH), and Superoxide dismutase (SOD) respectively.

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Characterization of Indonesian Tangerine Cultivar by Morphological and Issr Markers

Cited by 8 publications

References 0 publications

Genetic Diversity in Persian Lime (Citrus Latifolia Tanaka) Accessions Using Morphological and Molecular Markers

Genetic Diversity in Persian Lime (Citrus Latifolia Tanaka) Accessions Using Morphological and Molecular Markers

ASSESSMENT OF TAXONOMIC AFFINITY OF INDONESIAN PUMMELO (<i>CITRUS MAXIMA</i> (BURM.) MERR.) BASED ON MORPHOLOGICAL CHARACTERS

The Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions

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