Methanolic extracts from bulbs of three onion (Allium cepa L.) varieties: red (Pusa Red), white (Pusa White Round), and yellow (Arka Pitamber) and the CHCl 3 , Et 2 O, EtOAc, and n-BuOH fractions obtained from those extracts were investigated for their total phenolics and antioxidant activity. The antioxidant activity measurements, expressed as total antioxidant status (TAS), were 5.2-50.18, 2.2-35.6 and 3.5-42.2 mg ml −1 for red, white and yellow onion bulb extracts, respectively, as compared to trolox (IC 50 , 40.2 mg ml −1 ). The total phenolic content of the samples was 62-186, 56-156 and 68-165 mg GAE ml −1 for red, white and yellow onion bulb extracts, respectively, with all solvents. There was a positive correlation between the total phenolic content and antioxidant activity for MeOH extracts and EtOAc fractions (R 2 = 0.96, 0.8 respectively). Of the selected colored onion bulbs the red onion (IC 50 = 14.2 μg ml −1 ) revealed more potent xanthine oxidase inhibitory activity to those of yellow (IC 50 = 15.5 μg ml −1 ) and white onion (IC 50 = 17.0 μg ml −1 ) in vitro and as compared to control allopurinol (IC 50 = 10.5 μg ml −1 ). The kinetic study by xanthine oxidase revealed that the white and yellow onion extracts were non-competitive inhibitors, as K m values were constant, while V max consequently decreased with increased inhibitor concentration. In the red onion extract there was an increase in K m values while V max remained constant at all extract concentrations, thus confirming a competitive type of inhibition by the Lineweaver-Burk equation. These findings suggest that these onion varieties can be considered as potential sources of antioxidants and XO inhibitory agents.