Characterization of Insulin-Like Growth Factor Binding Protein-3 in Chronic Renal Failure Serum

Powell, David R.; Liu, Francis; Baker, Bonita K.; Lee, Philip D K; Belsha, Craig W.; Brewer, Eileen D.; Hintz, Raymond L.

doi:10.1203/00006450-199302000-00011

Cited by 64 publications

(36 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar comparisons for groups of subjects with GH disorders (all GH-deficient subjects, all acromegalic subjects) are shown in Table 3; again, correlation coefficients were high (in the range 0·82-0·98), and the slopes of regression lines for comparisons of Method 4 vs Method 1 were consistently lower than for comparisons between Methods 2 or 3 vs Method 1, consistent with a systematic effect of assay calibration for Method 4. The final comparisons were for samples from pregnant subjects and patients with chronic renal failure, both conditions where proteolysis of IGFBP-3 is reported to occur (Hossenlopp et al 1990, Powell et al 1993. In these relatively small sample groups, greater discrepancies among some of the methods were seen than in the other patient groups (Table 3).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Measurement of the acid-labile subunit of the insulin-like growth factor binding protein complex in human serum: a comparison of four immunoassays

Baxter

Svejkar²,

Khosravi³

et al. 2000

Journal of Endocrinology

View full text Add to dashboard Cite

The acid-labile subunit (ALS) of the high molecular weight insulin-like growth factor binding protein complex is a liver-derived glycoprotein which is regulated by growth hormone and serves as a serum marker of growth hormone action. We have compared the measurement of ALS by four immunoassay methods (two RIAs, two ELISAs) utilizing various polyclonal and monoclonal antibodies raised against natural or recombinant human ALS, or synthetic ALS peptides. Despite the variety of methodologies and reagents, results obtained by the four methods were highly correlated for 125 sera from various patient groups, and when compared for individual groups of sera from healthy children and adults, growth hormonedeficient children and adults, and subjects with acromegaly. Some weaker correlations among methods were seen when measuring ALS levels in groups of sera from pregnant subjects and subjects with chronic renal failure. An assay using antibodies raised against recombinant ALS yielded lower apparent values than the other methods in patient sera, the discrepancy probably being attributable to a difference in standardization. We conclude that a variety of assay formats and reagents can yield serum ALS values of potential clinical utility.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The functional abnormality in pregnancy IGFBP-3 is thus still controversial. In contrast, the proteolyzed form of IGFBP-3 seen in renal failure serum has an obvious functional abnormality since it circulates at a low molecular weight and fails to complex with ALS (Powell et al 1993 .…”

Section: Nsmentioning

confidence: 99%

Measurement of the acid-labile subunit of the insulin-like growth factor binding protein complex in human serum: a comparison of four immunoassays

Baxter

Svejkar²,

Khosravi³

et al. 2000

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…The circulating half-lives of IGF-I and IGF-II are reported to be about 10 min in the free form, less than 30 min in binary complexes, and 12-15 h in the ternary complexed form (6). IGFBP-3 has been extensively documented as being unique among the IGFBPs in its ability to form ternary complexes with the IGFs and ALS (4,(7)(8)(9)(10). These complexes are thus thought to form a circulating reservoir of IGFBP-3 and IGFs because, unlike free and binary complexed IGFs, they appear unable to undergo transcapillary passage to tissues and have a long circulating half-life, predominantly due to the stabilizing effect of ALS (11,12).…”

mentioning

confidence: 99%

Insulin-like Growth Factor (IGF)-binding Protein 5 Forms an Alternative Ternary Complex with IGFs and the Acid-labile Subunit

Twigg

Baxter

1998

Journal of Biological Chemistry

172

View full text Add to dashboard Cite

“…IGFBP-3 may accumulate in circulation in renal failure, due to decreased renal excretion [18,19]. IGF-I may be much more bound to IGFBP-3 under such condition as an increase in IGFBP-3 and that may result in a reduced free IGF-I level, which may cause growth retardation [1,10,20,21].…”

Section: Discussionmentioning

confidence: 99%

“…Thus it is necessary to investigate those values in the uremic children estimating by immunoblotting assay, cross-linking assay, RIA or chromatography methods of IGFBPs [18].…”

Section: Discussionmentioning

confidence: 99%

Changes in Serum Levels of IGF-I and IGFBP-3 in Children with Chronic Renal Failure.

et al. 1995

View full text Add to dashboard Cite

Abstract.The significance of serum insulin-like growth factor I (IGF-I) and insulin-like growth factor binding protein 3 (IGFBP-3) levels in uremia is still controversial.In this study we measured serum IGF-I by immunoradiometric assay (IRMA) and IGFBP-3 levels by specific radioimmunoassay (RIA) in 28 children (aged 3-16 years) with end-stage (n=14, on hemodialysis) and pre-terminal renal failure (n=14) and in 15 age-matched healthy children. Thyroid function of the patients was also investigated and GHstimulation tests with L-Dopa and insulin were performed. Neither IGF-I nor IGFBP-3 levels significantly correlated with mean height SDS for bone age or for chronological age in either non dialysis patients or pubertal (n=10) or prepubertal patients (n=18). These data was consistent with the concept that growth in CRF was not related to abnormalities in serum IGF-I or IGFBP-3 levels.

show abstract

Characterization of Insulin-Like Growth Factor Binding Protein-3 in Chronic Renal Failure Serum

Cited by 64 publications

References 40 publications

Measurement of the acid-labile subunit of the insulin-like growth factor binding protein complex in human serum: a comparison of four immunoassays

Measurement of the acid-labile subunit of the insulin-like growth factor binding protein complex in human serum: a comparison of four immunoassays

Insulin-like Growth Factor (IGF)-binding Protein 5 Forms an Alternative Ternary Complex with IGFs and the Acid-labile Subunit

Changes in Serum Levels of IGF-I and IGFBP-3 in Children with Chronic Renal Failure.

Contact Info

Product

Resources

About