Characterization of Inulin Hydrolyzing Enzyme(s) in Oleaginous Yeast Trichosporon cutaneum in Consolidated Bioprocessing of Microbial Lipid Fermentation

Wang, Juan; Zhang, Huizhan; Bao, Jie

doi:10.1007/s12010-015-1798-5

Cited by 8 publications

(6 citation statements)

References 33 publications

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“…T. fermentans CICC 1368 showed low lipid accumulation on pure molasses, but enzymatic hydrolysate supplementation of sweet potato vines increased lipid content (Shen et al 2015). Inulin can be directly utilized by T. cutaneum for microbial lipid fermentation without a hydrolysis step and 4.79 g/L of lipid has been produced from 50 g/L inulin (Wang et al 2015). The lipid productivity of the T. domesticum strain in DPW medium is similar to that for those cited.…”

Section: Biomass Yield and Lipid Contentsupporting

confidence: 61%

Deproteinated Potato Wastewater as a Sustainable Nitrogen Source in Trichosporon domesticum Yeast Lipids Biosynthesis—a Concept of Valorization of Wastewater from Starch Industry

Gientka

Aleksandrzak‐Piekarczyk

Bzducha-Wróbel

et al. 2019

Potato Res.

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This study determines the ability of an isolated Trichosporon domesticum yeast strain to accumulate intracellular lipids in media with deproteinated potato wastewater (DPW) containing various carbon sources. The yeast strain was isolated from kefir and identified by internal transcribed spacer (ITS) region sequence analysis. The sequence was deposited in GenBank under accession number MH094668, and the strain was deposited in Polish Collection of Microorganisms as T. domesticum PCM 2960. DPW is an inexpensive and valuable source of nitrogen, potassium, phosphorus, and other elements in yeast cultures. DPW supplemented with glucose medium was most effective at stimulating lipid biosynthesis by T. domesticum PCM 2960 and bioreactor incubation resulted in a final lipid yield of 4.8 g L −1. The lipids of the T. domesticum PCM 2960 biomass were characterized by high contents of linoleic acid (Δ9,12 C18:2), oleic acid (Δ9 C18:1), palmitic acid (C16:0), and α-linolenic acid (Δ9,12,15 C18:3). Theoretical calculations for biodiesel properties showed that the methylated esters of lipids from T. domesticum PCM 2960 biomass cannot be used as a biodiesel in diesel engines. Additionally, the ability to produce biofilm as one criterion for pathogenicity was tested. The ability for biofilm formation by the isolated strain was low. This study provides a promising solution for the more economical production of microbial lipids with DPW.

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Section: Biomass Yield and Lipid Contentsupporting

confidence: 61%

Deproteinated Potato Wastewater as a Sustainable Nitrogen Source in Trichosporon domesticum Yeast Lipids Biosynthesis—a Concept of Valorization of Wastewater from Starch Industry

Gientka

Aleksandrzak‐Piekarczyk

Bzducha-Wróbel

et al. 2019

Potato Res.

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“…These genetically engineered strains also showed an excellent ability to produce erythritol (120.9 g/L) and citric acid (105.2 g/L). In another oleaginous yeast Trichosporon cutaneum , it was found that inulin could be utilized directly for microbial lipid fermentation without a hydrolysis step ( Wang et al, 2015 ). Correspondingly, a consolidated bioprocessing technology for lipid production from inulin was developed and 4.79 g/L of lipid was produced from 50 g/L inulin.…”

Section: Engineering Oleaginous Yeasts For Production Of Fuels and Chmentioning

confidence: 99%

Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals

Shi

Zhao

2017

Front. Microbiol.

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Oleaginous yeasts have been increasingly explored for production of chemicals and fuels via metabolic engineering. Particularly, there is a growing interest in using oleaginous yeasts for the synthesis of lipid-related products due to their high lipogenesis capability, robustness, and ability to utilize a variety of substrates. Most of the metabolic engineering studies in oleaginous yeasts focused on Yarrowia that already has plenty of genetic engineering tools. However, recent advances in systems biology and synthetic biology have provided new strategies and tools to engineer those oleaginous yeasts that have naturally high lipid accumulation but lack genetic tools, such as Rhodosporidium, Trichosporon, and Lipomyces. This review highlights recent accomplishments in metabolic engineering of oleaginous yeasts and recent advances in the development of genetic engineering tools in oleaginous yeasts within the last 3 years.

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“…R. toruloides can also metabolise other economically relevant feedstocks including acetic acid [9], glycerol [10] and inulin [11], [12]. Here we report the genome sequence of R. toruloides strain CGMCC 2.1609 which has inulinase activity [11].…”

Section: Introductionmentioning

confidence: 95%

“…R. toruloides can metabolise the five- and six-carbon sugars liberated by degradation of lignocellulosic biomass, and can achieve a high lipid yield [7], [8]. R. toruloides can also metabolise other economically relevant feedstocks including acetic acid [9], glycerol [10] and inulin [11], [12]. Here we report the genome sequence of R. toruloides strain CGMCC 2.1609 which has inulinase activity [11].…”

Section: Introductionmentioning

confidence: 97%

Genome sequence of the oleaginous yeast Rhodotorula toruloides strain CGMCC 2.1609

et al. 2017

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Most eukaryotic oleaginous species are yeasts and among them the basidiomycete red yeast, Rhodotorula (Rhodosporidium) toruloides (Pucciniomycotina) is known to produce high quantities of lipids when grown in nitrogen-limiting media, and has potential for biodiesel production. The genome of the CGMCC 2.1609 strain of this oleaginous red yeast was sequenced using a hybrid of Roche 454 and Illumina technology generating 13 × coverage. The de novo assembly was carried out using MIRA and scaffolded using MAQ and BAMBUS. The sequencing and assembly resulted in 365 scaffolds with total genome size of 33.4 Mb. The complete genome sequence of this strain was deposited in GenBank and the accession number is LKER00000000. The annotation is available on Figshare (doi:10.6084/m9.figshare.4754251).

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Characterization of Inulin Hydrolyzing Enzyme(s) in Oleaginous Yeast Trichosporon cutaneum in Consolidated Bioprocessing of Microbial Lipid Fermentation

Cited by 8 publications

References 33 publications

Deproteinated Potato Wastewater as a Sustainable Nitrogen Source in Trichosporon domesticum Yeast Lipids Biosynthesis—a Concept of Valorization of Wastewater from Starch Industry

Deproteinated Potato Wastewater as a Sustainable Nitrogen Source in Trichosporon domesticum Yeast Lipids Biosynthesis—a Concept of Valorization of Wastewater from Starch Industry

Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals

Genome sequence of the oleaginous yeast Rhodotorula toruloides strain CGMCC 2.1609

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