Characterization of iron-reducing <i>Alteromonas putrefaciens</i> strains from oil field fluids

Semple, Kathleen M.; Westlake, D. W. S.

doi:10.1139/m87-064

Cited by 135 publications

(105 citation statements)

References 21 publications

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“…Fe(III)-and Mn(IV)-reducing microorganisms have been enumerated and cultured on a wide variety of solid, semisolid, and liquid media that are similar in composition to those used for other heterotrophic microorganisms but with the modification that some form of Fe(III) or Mn(IV) was included in the medium. Naturally occurring Fe(III) or Mn(IV) oxides have been employed, as have hematite, goethite, lepidocrocite, synthetic poorly crystalline Fe(III) oxides, FePO4, FeCl3, and Fe(III) citrate (17,45,91,116,155,172,198,236,246,256,282,285,318). For agar media, the Fe(III) or Mn(IV) may be added as an overlay (89,230).…”

Section: Enumeration and Culturing Of Fe(hi) And Mn(iv) Reducersmentioning

confidence: 99%

“…This technique may be inappropriate for monitoring Fe(III) reduction in mixed cultures in media which contain significant quantities of sulfate, because sulfate reduction could lead to the formation of black iron sulfides, some of which are magnetic. In liquid medium with Fe(III)-citrate as the electron acceptor, the medium changes from orange-brown to green as Fe(III) is reduced (198,282). At the completion of Fe(III) reduction, the medium typically clears because the Fe(II) forms vivianite [Fe3(PO4)2 -8H20], a white precipitate (201).…”

Section: Enumeration and Culturing Of Fe(hi) And Mn(iv) Reducersmentioning

confidence: 99%

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Dissimilatory Fe(III) and Mn(IV) Reduction

Lovley

Holmes

Nevin

2004

Advances in Microbial Physiology

1,484

1,061

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Section: Enumeration and Culturing Of Fe(hi) And Mn(iv) Reducersmentioning

confidence: 99%

Section: Enumeration and Culturing Of Fe(hi) And Mn(iv) Reducersmentioning

confidence: 99%

Dissimilatory Fe(III) and Mn(IV) Reduction

Lovley

Holmes

Nevin

2004

Advances in Microbial Physiology

1,484

1,061

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“…shewanella.org). Some Shewanella strains are also able to degrade pollutants such as chlorinated solvents (Petrovskis et al, 1994), petroleum (Semple & Westlake, 1987) and RDX (1,3,5-trinitroperhydro-1,3,5-triazine) (Zhao et al, 2004), some can produce polyunsaturated fatty acids (Bowman et al, 1997;Russell & Nichols, 1999;Satomi et al, 2003) and some are able to grow under extreme conditions (Bozal et al, 2002;Kato et al, 1998;Nogi et al, 1998;Stapleton et al, 2005).…”

mentioning

confidence: 99%

Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean

Gao

Obraztova

Stewart

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

109

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A novel marine bacterial strain, PV-4 T , isolated from a microbial mat located at a hydrothermal vent of Loihi Seamount in the Pacific Ocean, has been characterized. This micro-organism is orangey in colour, Gram-negative, polarly flagellated, facultatively anaerobic and psychrotolerant (temperature range, 0-42 6C). No growth was observed with nitrate, nitrite, DMSO or thiosulfate as the electron acceptor and lactate as the electron donor. The major fatty acid detected in strain PV-4 T was iso-C 15 : 0 . Strain PV-4 T had ubiquinones consisting mainly of Q-7 and Q-8, and possessed menaquinone MK-7. The DNA G+C content of the strain was 53?8 mol% and the genome size was about 4?5 Mbp. Phylogenetic analyses based on 16S rRNA gene sequences placed PV-4 T within the genus Shewanella. PV-4 T exhibited 16S rRNA gene sequence similarity levels of 99?6 and 97?5 %, respectively, with respect to the type strains of Shewanella aquimarina and Shewanella marisflavi. DNA from strain PV-4 T showed low mean levels of relatedness to the DNAs of S. aquimarina (50?5 %) and S. marisflavi (8?5 %). On the basis of phylogenetic and phenotypic characteristics, the bacterium was classified in the genus Shewanella within a distinct novel species, for which the name Shewanella loihica sp. nov. is proposed. The type strain is PV-4 T (=ATCC BAA-1088 T =DSM 17748 T ).The genus Shewanella consists of rod-shaped, Gramnegative, facultatively anaerobic, readily cultivated gammaproteobacteria (Gauthier et al., 1995;MacDonell & Colwell, 1985;Venkateswaran et al., 1999). While many Shewanella strains remain uncharacterized, there are 32 recognized Shewanella species: the latter were isolated from a variety of sources, primarily aquatic environments and sediments (Bowman et al., 1997;Bozal et al., 2002;Brettar et al., 2002;Coyne et al., 1989;Ivanova et al., 2001 Ivanova et al., , 2003a Ivanova et al., , b, 2004aLeonardo et al., 1999;Makemson et al., 1997;Nogi et al., 1998;Nozue et al., 1992;Satomi et al., 2003Satomi et al., , 2006Skerratt et al., 2002; Toffin et al., 2004;Venkateswaran et al., 1998Venkateswaran et al., , 1999Xu et al., 2005; Yoon et al., 2004a, b;Zhao et al., 2005Zhao et al., , 2006Ziemke et al., 1998). The bacteria of this genus have attracted great attention because of their diverse respiratory capacities, illustrated by their ability to utilize a wide range of terminal electron acceptors, including oxygen, nitrate, metals and sulfur compounds (Kostka et al., 1996;Myers & Nealson, 1988;Venkateswaran et al., 1999; http://www. shewanella.org). Some Shewanella strains are also able to degrade pollutants such as chlorinated solvents (Petrovskis et al., 1994), petroleum (Semple & Westlake, 1987) and RDX (1,3,5-trinitroperhydro-1,3,5-triazine) (Zhao et al., 2004), some can produce polyunsaturated fatty acids (Bowman et al., 1997;Russell & Nichols, 1999;Satomi et al., 2003) and some are able to grow under extreme conditions (Bozal et al., 2002;Kato et al., 1998;Nogi et al., 1998;Stapleton et al., 2005).In a previous study, several She...

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“…It can thus be concluded on the basis of moderate 16s rDNA and DNA-DNA similarity values that strain SB2BT represents a novel species within the genus Shewanella. Though many Shewanella isolates share common phenotypic traits, the 16s rDNA (Fonnesbech-Vogel et al, 1997) and DNA-DNA hybridization studies (Owen et al, 1978;Semple & Westlake, 1987) have revealed the obvious heterogeneity of various strains that have been grouped within the S. putrefaciens group. This paper also supports the fact that without nucleotide sequence analysis, strain SB2BT would have been placed within S. putrefaciens.…”

mentioning

confidence: 99%

Shewanella amazonensis sp. nov., a novel metal-reducing facultative anaerobe from Amazonian shelf muds

Venkateswaran

Dollhopf

Aller

et al. 1998

International Journal of Systematic Bacteriology

119

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A new bacterial species belonging t o the genus Shewanella is described on the basis of phenotypic characterization and sequence analysis of its 165 rRNAencoding and gyrase B (gyrB) genes. This organism, isolated from shallowwater marine sediments derived from the Amazon River delta, is a Gramnegative, motile, polarly flagellated, facultatively anaerobic, rod-shaped eubacterium and has a G+C content of 51.7 mol0/o. Strain SB2BT is exceptionally active in the anaerobic reduction of iron, manganese and sulfur compounds. SB2BT grows optimally at 35 "C, with 1-3% NaCl and over a pH range of 7-8. Analysis of the 165 rDNA sequence revealed a clear affiliation between strain SB2BT and members of the gamma subclass of the class Proteobacteria. High similarity values were found with certain members of the genus Shewanella, especially with Shewanella putrefaciens, and this was supported by cellular fatty acid profiles and phenotypic characterization. DNA-DNA hybridization between strain SB2BT and its phylogenetically closest relatives revealed low similarity values (246-4207 %) which indicated species status for strain SB2BT. That SB2BT represents a distinct bacterial species within the genus Shewanella is also supported by gyrB sequence analysis. Considering the source of the isolate, the name Shewanella amazonensis sp. nov. is proposed and strain SB2BT (= ATCC 7003293 is designated as the type strain.

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Characterization of iron-reducing Alteromonas putrefaciens strains from oil field fluids

Cited by 135 publications

References 21 publications

Dissimilatory Fe(III) and Mn(IV) Reduction

Dissimilatory Fe(III) and Mn(IV) Reduction

Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean

Shewanella amazonensis sp. nov., a novel metal-reducing facultative anaerobe from Amazonian shelf muds

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