Characterization of JAK2 V617F (1849 G > T) Mutation in Cervical Cancer Related to Human Papillomavirus and Sexually Transmitted Infections

Abstract

BackgroundHuman papillomavirus (HPV) genotypes that infect the genital tract play a main etiologic role in cervical cancer progression. Other environmental factors, such as sexually transmitted diseases and the host genetic pattern, contribute to infection persistence of the uterus and cervical epithelium in sustaining their malignancy. The Janus kinase 2 is a non-receptor tyrosine kinase in cell signaling process of tumor genesis. In the present study, JAK2 V167F mutation was distinguished in women with sexually transmitted infections, such as Herpes simplex virus 2, Chlamydia trachomatis and Mycoplasma genitalium and cervical cancer.MethodsThis case-control survey was performed on 195 liquid based cytology of women specimens. Fifty, 98, and 47 samples were from women with known cervical cancer, HPV positive and HPV negative, respectively. Single nucleotide polymorphism analysis, sexually transmitted infections detection and HPV genotyping were carried out using approved PCR- RFLP, in-house multiplex TaqMan Real Time PCR and the reverse dot blot hybridization assay.ResultsHPVs 6, 16, 18, 11, 31, and 51 were the most common genotypes. The prevalence rate of multiple HPV genotypes was 46.0% to 10.1%. Analysis of JAK2 V617F (1849 G > T) showed that prevalence of mutation was GG (65.1%), GA (34.9%), and TT (0%), respectively. There were no statistically significant differences between this mutation and variables of population survey (P ≥ 0.05).ConclusionsThe molecular epidemiology study on the genetic polymorphisms, i.e., JAK2 V617F and other single nucleotide polymorphisms as a diagnostic tool is necessary for cancer screening and prophylactic programs.

The Impact of MTHFR 1298 A > C and 677 C > T Gene Polymorphisms as Susceptibility Risk Factors in Cervical Intraepithelial Neoplasia Related to HPV and Sexually Transmitted Infections

“…We performed a detailed HPV genotyping and pathogen detection of STIs such as C. trachomatis, HSV2 and M. genitalium in our previous studies [13,14]. The details of data are not shown.…”

“…In a MTHFR 1298, PCR–RFLP was expected to produce 145 bp as undigested, 77, 37 and 29 bp as wild type, 108, 77, 37 and 29 bp as heterozygous and 108 bp as homozygous. In addition, in a MTHFR 677 PCR–RFLP was expected to produce 200 bp as undigested, 200 bp as wild type, 200 and 175 bp as heterozygous and 175 bp as homozygous [ 12 , 13 ].…”

CXCL1, CXCL10 and CXCL12 Chemokines are Variously Expressed in Acute Myeloid Leukemia Patients Prior and Post Bone Marrow Transplantation

“…CXCL1 was markedly decreased following BMT and this reduced level is perhaps due to decreased cellular sources of this chemokine because this chemokine is produced by several BM resident and migratory cell types (Abdolmaleki and Sohrabi, 2018). One of the possible mechanisms which may explain the decreased levels of both CXCL1 and CXCL10 following BMT, is their nature as inducible chemokine.…”

"JAK2 V617F Mutation in Cervical Cancer Related to HPV & STIs" - Letter

“…In a recent issue of the Journal of Cancer Prevention , Abdolmaleki and Sohrabi investigated the frequency of the JAK2 V617F mutation in patients with cervical cancer, proposing that polymorphisms in genes encoding elements of the intracellular JAK-STAT signalling pathway may contribute to oncogenesis through an immunomodulatory effect [1]. Several aspects of this study require extensive explanation and clarification.…”