Characterization of kinetic, thermodynamic, and binding properties of l-phenylalanine molecularly imprinted polymer

Hroboňová, Katarína; Vybohová, Veronika; Lomenová, Anna; Špačková, Andrea; Svitková, Veronika

doi:10.1007/s00706-022-02900-4

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…Briefly, L-phenylalanine (0.35 mmol), methacrylic acid (2 mmol), and ethylene glycol dimethacrylate, were dissolved in a mixture of toluene, glacial acetic acid, and trifluoroacetic acid in a falcon-tube. The addition of glacial acetic acid and trifluoroacetic acid facilitates the dissolution of L-phenylalanine in the porogenic solvent of toluene [ 17 ]. Then, the polymerization initiator, 1,1′-azobiscyclohexanecarbonitrile, was added, and the homogeneous solution was purged with nitrogen for five minutes (min) before sealing the tube.…”

Section: Methodsmentioning

confidence: 99%

Molecularly Imprinted Polymers Specific towards 4-Borono-L-phenylalanine—Synthesis Optimization, Theoretical Analysis, Morphology Investigation, Cytotoxicity, and Release Studies

Balcer,

Sobiech,

Giebułtowicz

et al. 2023

Polymers

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The aim of this study was to create molecularly imprinted polymers (MIPs) that are specific towards 4-borono-L-phenylalanine (BPA) to serve as boron compound carriers. The honeycomb-like MIPs were characterized in the matter of adsorption properties, morphology, structure, and cytotoxicity towards A549 and V79-4 cell lines. The honeycomb-like MIP composed from methacrylic acid and ethylene glycol dimethacrylate was characterized by a binding capacity of 330.4 ± 4.6 ng g−1 and an imprinting factor of 2.04, and its ordered, porous morphology was confirmed with scanning electron microscopy. The theoretical analysis revealed that the coexistence of different anionic forms of the analyte in basic solution might lower the binding capacity of the MIP towards BPA. The release profiles from the model phosphate buffer saline showed that only 0 to 4.81% of BPA was released from the MIP within the time frame of two hours, furthermore, the obtained material was considered non-cytotoxic towards tested cell lines. The results prove that MIPs can be considered as effective BPA delivery systems for biomedical applications and should be investigated in further studies.

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Section: Methodsmentioning

confidence: 99%