Characterization of Klebsiella granulomatis pathogenic to silkworm, Bombyx mori L.

Abstract

Bacterial disease of silkworm causes significant reduction of silk production leading to huge economic loss. This study aims to isolate bacteria from diseased silkworm and to determine its pathogenicity and antibiotic resistance. A strain of Klebsiella granulomatis has been isolated from silkworm haemolymph which was later identified on the basis of biochemical tests and 16S rRNA gene sequencing. The optimum culture condition of K. granulomatis was determined at pH 7.0 and 37 °C temperature. The strain was resistant to most of the antibiotics used in this study except azithromycin, gentamycin and ciprofloxacin. The strain is capable to reproduce flacherrie like symptoms with high mortality rate when re-injected into healthy silkworm. Treatment with low dose of ciprofloxacin was found to be effective to prevent flacherrie induced by the isolated K. granulomatis strain.

Characterization and pathogenicity assessment of gut-associated microbes of muga silkworm Antheraea assamensis Helfer (Lepidoptera: Saturniidae)

“…This result was in agreement with the earlier reports as demonstrated in bacterial infections in other silkworms (Choudhury et al, 2004;Mohanta et al, 2014;Jurat-Fuentes and Jackson, 2012). It was further found that pathogens could kill 50 to 90% of silkworms within four days either by injection or oral administration of the inoculums.…”

The Interaction between Arbuscular Mycorrhizal Fungi and Endophytic Bacteria Enhances Plant Growth of Acacia gerrardii under Salt Stress

“…Then, the top phase was transferred to a fresh tube, and the DNA was precipitated with isopropanol and later re-suspended in TE buffer (pH 7.4). Next, 16S rDNA was amplified by polymerase chain reaction (PCR) using universal forward 16SF (5′-GAGTTTGATCCTGGCTCAG-3′) and reverse 16SR (5′-GAAAGGAGGTGATCCAGCC-3′) primers (Mohanta et al, 2015 ). The PCR reactions were 25 μl and contained 5 μl of 5 × buffer (TaKaRa Bio Inc.), 0.5 μl of dNTP mixture (10 mM of each dNTP, TaKaRa Bio Inc.), 1 μl of 2% BSA (Promega), 0.5 μl of forward primer (10 μM), 0.5 μl of reverse primer (10 μM), 0.125 μl of One Taq DNA Polymerase (New England Biolabs), 15.375 MQ and 2 μl of template DNA.…”

Identification and Characterization ofBacillus cereusSW7-1 inBombyx mori(Lepidoptera: Bombycidae)

“…The symptoms of the silkworms were observed and the mortality of the silkworms was recorded. Data were analyzed using SPSS 20, and the median lethal concentration (LC 50 ) was obtained ( Mohanta et al. 2014 ).…”