Characterization of lake minnow <i>Eupallasella percnurus</i> semen in relation to sperm morphology, regulation of sperm motility and interpopulation diversity

Dietrich, G.J.; Pecio, Anna; Sikorska, Justyna; Wolnicki, Jacek; Słowińska, Mariola; Hliwa, Piotr; Kamiński, Rafał; Andronowska, Aneta; Ciereszko, Andrzej

doi:10.1111/jfb.12435

Cited by 7 publications

(4 citation statements)

References 23 publications

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“…In lake minnow ( Eupallasella percnurus ), the two populations analysed were markedly different in several sperm quality biomarkers such as milt volume, sperm concentration and sperm motility (Dietrich et al . ). As a result, authors estimated 134 million motile spermatozoa per ejaculate (volume × concentration × percentage of motility) in the ‘Siedliszcze’ population compared to 480 million motile sperm in males from ‘Guzy’ population.…”

Section: Fish Sperm Motility: a Useful Tool For Multidisciplinary Stumentioning

confidence: 97%

Fish sperm motility assessment as a tool for aquaculture research: a historical approach

Gallego

Asturiano

2018

Reviews in Aquaculture

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Fish sperm motility is nowadays considered the best biomarker for the quality of fish spermatozoa, and sperm motion parameters from more than 300 fish species have been reported in more than 1500 scientific articles covering a wide range of topics, from molecular biology to ecology. The most studied topics have been (i) the sperm storage (involving both the use of chilled-storage protocols for shortterm periods and sperm cryopreservation techniques for long-term storage), (ii) the sperm physiology (fathom in the spermatozoa activation process and the whole propulsion machinery of the sperm cells) and (iii) the broodstock management (covering aspects such as rearing conditions, dietary requirements or hormonal induction treatments). In addition, other aquaculture and ecological topics, such as (iv) the knowledge of the breeding cycle of the species, (v) the phenomenon of the sperm competition and (vi) ecotoxicological studies for the evaluation of aquatic environments, have also been approached from the evaluation of sperm motion performance. Therefore, fish sperm motility assessment can serve as a potential tool for aquaculture and ecological purposes, covering key topics of fundamental and applied research. This review gives an overview of the major research areas in which fish sperm motility has been applied successfully.

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Section: Fish Sperm Motility: a Useful Tool For Multidisciplinary Stumentioning

confidence: 97%

Fish sperm motility assessment as a tool for aquaculture research: a historical approach

Gallego

Asturiano

2018

Reviews in Aquaculture

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“…Sperm motility characteristics were measured using a two-step procedure described by Wojtczak et al (2007) and modified by Dietrich et al (2014). Motility (MOT) and straight line velocity (VSL) were determined.…”

Section: Introductionmentioning

confidence: 99%

Hormonal treatment with Ovopel increases sperm production in lake minnow, Eupallasella percnurus (Pallas, 1814)

Kamiński

Judycka

Sikorska

et al. 2021

Fisheries &Amp; Aquatic Life

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The aim of the study was to determine the suitability of Ovopel, which contains an mGnRH analog and metoclopramide (a dopamine inhibitor), for the stimulation of spermiation in the cyprinid fish lake minnow, Eupallasella percnurus (Pallas), which is endangered with extinction in Poland. The hormonal treatment effected an approximate twofold increase in sperm volume with a simultaneous decrease in sperm concentration and negligible differences in motility and straight-line velocity.

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“…In relation to morphology study, the analyses carried out revealed that the spermatozoa of the four species studied presented a morphology similar to that of other cyprinids and leuciscids with external fertilisation, with small sized cells, spherical head, a middle piece and uni agellate (Neznanova, 2012). The sperm morphology of other leuciscid and cyprinid species has been characterised, for example, in E. percnurus, where the sperm head is 1.88 µm long and 2.03 µm wide (Dietrich et al, 2014)…”

Section: Sperm Quantity and Qualitymentioning

confidence: 82%

Assessment of male reproductive traits in endangered leuciscids from the Iberian Peninsula: first attempts to store gametes both at short- and long- term

Hernández-Rodríguez

Sousa‐Santos

Gil

et al. 2023

Preprint

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During the spring of 2022 several endangered leuciscid species (Anaecypris hispanica, Squalius aradensis, Anachondrostoma. occidentale and Iberochondrostoma lusitanicum) were sampled both at the Vasco da Gama aquarium facilities and in some rivers of the Algarve region, Portugal. Sperm samples were extracted by gentle abdominal pressure and sperm motion parameters were assessed for the first time in four species, using a computerised analysis system. The results obtained showed that spermatozoa kinetic patterns were similar for all 4 species, with high motility and velocity values after the sperm activation time and with a marked decrease after 20 seconds. On the other hand, sperm longevity was highly variable between species, with short longevities (around 40 s) for A. hispanica and S. aradensis, and longer longevities (100–120 s) for A. occidentale and I. lusitanicum, which could indicate a latitudinal pattern in terms of sperm longevity. At the same time, morphometric analysis was carried out for the four target species, revealing that spermatozoa showed similar sizes and shapes to other external fertilizers belonging to Leuscididae, with small spherical heads, uniflagellate and without acrosomes. In addition, different short-term gamete storage trails were performed by diluting sperm in 1:9 (sperm:extender) and storing them at 4ºC. Although the results obtained were uneven among the species studied, the dilution and extender used generated motilities above 40% up to day 4 of storage in S. aradensis and I. lusitanicum, and up to day 1–2 in A. hispanica and A. occidentale, respectively. Finally, gamete cryopreservation trials were also carried on these threatened species. Although cryopreserved samples showed significantly lower motility than fresh samples, some protocols generate acceptable percentages of viability, DNA integrity, and sperm motility in some species such as I. lusitanicum and A. occidentale. The data revealed that the protocol based on 10% DMSO plus 7.5% egg yolk generated the best results. This study is the first to assess the reproductive traits of wild and captive populations of endangered leuciscids endemic from the Iberian Peninsula, describing the spermatozoa kinetics and developing protocols for managing male gametes both in short- and long- term storage. Outcomes will provide new and useful tools to complement the management and conservation of ex-situ breeding programs that are being developed on these four endangered species.

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Characterization of lake minnow Eupallasella percnurus semen in relation to sperm morphology, regulation of sperm motility and interpopulation diversity

Cited by 7 publications

References 23 publications

Fish sperm motility assessment as a tool for aquaculture research: a historical approach

Fish sperm motility assessment as a tool for aquaculture research: a historical approach

Hormonal treatment with Ovopel increases sperm production in lake minnow, Eupallasella percnurus (Pallas, 1814)

Assessment of male reproductive traits in endangered leuciscids from the Iberian Peninsula: first attempts to store gametes both at short- and long- term

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