Characterization of Leishmania colombiensis Sp. N (Kinetoplastida: Trypanosomatidae), a New Parasite Infecting Humans, Animals, and Phlebotomine Sand Flies in Colombia and Panama

Kreutzer, Richard D.; Corredor, Augusto; Grimaldi, Gabriel; Grögl, Max; Rowton, Edgar; Young, David W.; Morales, Alberto; McMahon-Pratt, Diane; Guzmán, Hilda; Tesh, Robert B.

doi:10.4269/ajtmh.1991.44.662

Cited by 80 publications

(67 citation statements)

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“…[9][10][11][12] L. colombiensis was isolated from four sand flies and a sloth in Panama. 13 Two other Leishmania species have been reported in Panama but never isolated from humans. One of them, the L. hertigi complex, has as its natural reservoir host the porcupine Coendou rothschildi , with a very high rate of infection (89%).…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of American Tegumentary Leishmaniasis in Panama

Miranda¹,

Carrasco²,

Paz³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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Abstract. American tegumentary leishmaniasis is an increasing public health problem in Panama. This study describes the clinical characteristics and the molecular epidemiology of leishmaniasis in Panama over a 5-year period (2004)(2005)(2006)(2007)(2008). Additionally, we applied a polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) -based assay to identify Leishmania species in clinical isolates, skin scrapings, and sandflies specimens. Whereas 60.3% of cases were detected with conventional parasitologic techniques (smear or in vitro culture), the PCR detected 72% positive patients. Our clinical-epidemiologic data corroborate the high incidence of L. ( Viannia ) panamensis and provide evidence of peridomestic and/or domestic transmission. Mucosal involvement was observed in 4.2% of the patients. The overall natural infection rate with Leishmania in 103 pools of sandflies was 0.46%. Lutzomyia gomezi and Lutzomya panamensis were the prevalent species incriminated as vectors at the capture sites in central Panama. This study contributes to a better knowledge of the current epidemiology of tegumentary leishmaniasis in Panama.

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Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of American Tegumentary Leishmaniasis in Panama

Miranda¹,

Carrasco²,

Paz³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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“…A new species, L. colombiensis, was isolated from four sand flies and a sloth in Panama, and from two humans and a sand fly in Colombia. 3 With regard to the remaining two species indigenous to Panama, L. hertigi appears to be host-specific for the porcupine Coendou rothschildi, and L. aristidesi is known only from a single focus in a coastal forest in eastern Panama from rodents and a single marsupial and has never been isolated from humans. 4,5 Identification of Leishmania species at the GML has been based upon in vitro characterization, pathologic manifestations in the golden hamster, and cellulose acetate isoenzyme electrophoesis.…”

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confidence: 99%

Short report epidemiologic studies on cutaneous leishmaniasis in eastern Panama.

Christensen¹,

Vasquez²,

Petersen³

1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. The Panamanian Ministry of Health, through the Interamerican Development Bank, contracted the Gorgas Memorial Laboratory to conduct epidemiologic studies on leishmaniasis and malaria in eastern Panama from July 1984 through June 1985. Preliminary results of the biomedical and entomologic teams investigating the epidemiology of cutaneous leishmaniasis in the eastern part of the country are presented in this short report. The principal findings of the study revealed 1) a large disparity in the incidence and prevalence of the disease among the five communities investigated; 2) the appearance of self-cures without the benefit of effective treatment; 3) a relatively high percentage of subclinical cases; and 4) determination of the sandfly vector species for each community. Also reported here is a case of a double infection with two distinct species of Leishmania, L. mexicana and L. amazonensis, in a single individual.The present study investigated epidemiologic parameters of cutaneous leishmaniasis among five communities in eastern Panama in which week-long surveys were conducted. This study was reviewed and approved by the Panamanian Ministry of Health. Four of these study sites (San Miguel, Torti, Santa Fe, and Meteti) are adjacent to the Interamerican Highway (Figure 1). The fifth community (Boca de Sabalo) is located in the extreme southeastern part of the country, and was accessed by boat via an extensive river system (Figure 1). Although the primitive working conditions in the communities precluded isolation and characterization of the parasite, it was assumed that all of the cases of leishmaniasis encountered were due to Leishmania panamensis since it has been the predominant species identified from human cases in Panama. However, several months after the termination of the project, one of the investigators (JLP) developed a lesion from which a biopsy specimen was obtained and found to be caused by L. amazonensis. 1 The locality in which the infection occurred could not be determined since this investigator traveled extensively throughout Panama during the study period.In 1977, several U.S. soldiers conducting jungle exercises near the Caribbean entrance to the Panama Canal developed leishmaniasis identified by personnel at Gorgas Memorial Laboratory (GML) as caused by L. amazonensis.1,2 In October 1986, the first indigenous case of infection with L. mexicana in Panama was isolated from one of two lesions from a young mestizo individual involved in hunting and agriculture in the northern and eastern part of the country. 1 Eight subsequent clones of parasites from each lesion revealed a double infection with L. mexicana and L. amazonensis. A new species, L. colombiensis, was isolated from four sand flies and a sloth in Panama, and from two humans and a sand fly in Colombia. 3 With regard to the remaining two species indigenous to Panama, L. hertigi appears to be host-specific for the porcupine Coendou rothschildi, and L. aristidesi is known only from a single focus in a coastal forest in eastern Panam...

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“…The 2 negative Venezuelan VL isolates had been previously identified as L. colombiensis, 7 a parasite included within the Viannia subgenus 18 and suspected of being common in western Venezuela endemic areas (Bonfante R, unpublished data). It is interesting that in one of the endemic areas, which neighbors a L. colombiensis-endemic area, only L. donovani-related parasites were detected.…”

Section: Resultsmentioning

confidence: 99%

Detection of Leishmania causing visceral leishmaniasis in the Old and New Worlds by a polymerase chain reaction assay based on telomeric sequences.

Chiurillo¹,

Sachdeva²,

Dole³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. We present a new polymerase chain reaction assay based on telomeric sequences of Leishmania donovani. When this assay was used in dilutions of purified L. donovani DNA, a strong amplification signal was observed with 1 fg of DNA. In a specificity test that used purified DNA from Old World and New World Leishmania, the assay recognized all parasites isolated from patients with visceral leishmaniasis, except for 2 isolates of Leishmania colombiensis from Venezuela and 1 isolate from Brazil. All Leishmania major and Leishmania tropica isolates tested were negative, except for one isolate in each species. We also used the assay on fresh and archive bone marrow samples recovered from Giemsa-stained slides and from dried blood stains.

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Characterization of Leishmania colombiensis Sp. N (Kinetoplastida: Trypanosomatidae), a New Parasite Infecting Humans, Animals, and Phlebotomine Sand Flies in Colombia and Panama

Cited by 80 publications

References 0 publications

Molecular Epidemiology of American Tegumentary Leishmaniasis in Panama

Molecular Epidemiology of American Tegumentary Leishmaniasis in Panama

Short report epidemiologic studies on cutaneous leishmaniasis in eastern Panama.

Detection of Leishmania causing visceral leishmaniasis in the Old and New Worlds by a polymerase chain reaction assay based on telomeric sequences.

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