Characterization of Ligand Shell for Mixed-Ligand Coated Gold Nanoparticles

Ong, Quy K.; Luo, Zhi; Stellacci, Francesco

doi:10.1021/acs.accounts.7b00165

Cited by 94 publications

(103 citation statements)

References 52 publications

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“…This endeavor is limited by our ability to fully investigate the nanoscale realm: Different characterization techniques are based on different physical properties, therefore only providing a partial picture of the nanoparticle characteristics. Making matters more challenging yet, the characterization methods themselves can directly affect the measured quantities …”

Section: Introductionmentioning

confidence: 99%

Nanoparticle Characterization: What to Measure?

et al. 2019

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What to measure? is a key question in nanoscience, and it is not straightforward to address as different physicochemical properties define a nanoparticle sample. Most prominent among these properties are size, shape, surface charge, and porosity. Today researchers have an unprecedented variety of measurement techniques at their disposal to assign precise numerical values to those parameters. However, methods based on different physical principles probe different aspects, not only of the particles themselves, but also of their preparation history and their environment at the time of measurement. Understanding these connections can be of great value for interpreting characterization results and ultimately controlling the nanoparticle structure–function relationship. Here, the current techniques that enable the precise measurement of these fundamental nanoparticle properties are presented and their practical advantages and disadvantages are discussed. Some recommendations of how the physicochemical parameters of nanoparticles should be investigated and how to fully characterize these properties in different environments according to the intended nanoparticle use are proposed. The intention is to improve comparability of nanoparticle properties and performance to ensure the successful transfer of scientific knowledge to industrial real‐world applications.

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Section: Introductionmentioning

confidence: 99%

Nanoparticle Characterization: What to Measure?

et al. 2019

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“…To determine whether our NPs could decorate fibrils that do not present clear twisted morphologies, we incubated NPs with transactive response DNA--binding protein 43 (TDP-- 43) fibrils, which is implicated in several neurodegenerative conditions including amyotrophic lateral sclerosis (ALS), subsets of frontotemporal dementia (FTLD--TDP) and AD (44). We were able to obtain uniform decoration with MUS:OT A NPs across the entirety of the fibrils ( edges are easily discerned from the background, allowing for immediate determination of the type of morphological polymorph.…”

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confidence: 99%

Unraveling the Complexity of Amyloid Polymorphism Using Gold Nanoparticles and Cryo-EM

Cendrowska

Silva

Ait-Bouziad

et al. 2019

Preprint

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The ability of proteins to self--assemble into different types of fibrils with distinct morphologies has been linked to the pathological and clinical heterogeneity of amyloid diseases such as Alzheimer's and Parkinson's disease. Herein, we describe novel nanoparticles (NPs) that efficiently label amyloid fibrils produced in vitro or isolated from postmortem tissues, under hydrating conditions and in such a way to unmask their polymorphism and morphological features. Using these NPs, we show that pathological aggregates exhibit exceptional morphological homogeneity compared to amyloid fibrils produced in vitro, consistent with the emerging view that the physiologic milieu is a key determinant of amyloid fibril strains. These advances pave the way for elucidating the structural basis of amyloid strains and toxicity. AbstractThe misfolding and self--assembly of proteins into β--sheet--rich amyloid fibrils of various structures and morphologies is a hallmark of several neurodegenerative and systemic diseases. Increasing evidence suggests that amyloid polymorphism gives rise to different strains of amyloids with distinct toxicity and pathology--spreading properties. Validating this hypothesis is challenging due to a lack of tools and methods that allow for the direct characterization of amyloid polymorphism in hydrated and complex biological samples. Here, we report on the use of 11--mercapto--1--undecanesulfonate--coated gold nanoparticles (NPs) to label the edges of synthetic, recombinant and native amyloid fibrils to assess amyloid morphological polymorphism using cryogenic transmission electron microscopy (cryo--EM).The fibrils studied were derived from amyloid proteins involved in disorders of the central nervous system (amyloid--β, tau, α--synuclein) and in systemic amyloidosis (a fragment of an immunoglobulin λ light chain). The labeling efficiency enabled imaging and characterization of amyloid fibrils of different morphologies under hydrated conditions using cryo--EM. These NPs allowed for the visualization of morphological features that are not directly observed using standard imaging techniques, including TEM with use of the negative stain or cryo--EM imaging. We also demonstrate the use of these NPs to label native paired helical filaments (PHFs) from the postmortem brain of an Alzheimer's disease patient, as well as amyloid fibrils extracted from the heart tissue of a patient suffering from systemic amyloid light--chain (AL) amyloidosis. Analysis of the cryo--EM images of amyloids decorated with NPs shows

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“…With MOF as a stabilizing template, UVvisible irradiation can induce reduction without additional or stabilizing ligands that typically degrade device performance. [67][68][69] Figure 1. MOFs as nano-reactors for the growth of Au NPs via photo-reduction.…”

Section: Resultsmentioning

confidence: 99%