Characterization of Linear and Chemically Cross-linked Hyaluronic acid using Various Analytical Techniques Including FTIR, ESI-MS, H1 NMR, and SEM

Al-Sibani, Mohammed; Al‐Harrasi, Ahmed; Rhh, Neubert

doi:10.16966/2576-5833.115

Cited by 7 publications

(8 citation statements)

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“…3C). The peak observed at around 2900 cm −1 in the HAM represented the C-H stretching after cross-linking 33,34 . Another additional small peak at about 1300 cm −1 in the HAM also confirmed Figure 1.…”

Section: Characterization Of Ham Water Absorption Capacity and Opticmentioning

confidence: 99%

Development of a novel hyaluronic acid membrane for the treatment of ocular surface diseases

Kim

Jung

Pak

et al. 2021

Sci Rep

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Ocular surface diseases (OSD) can cause serious visual deterioration and discomfort. Commercial artificial tear solution containing hyaluronic acid (HA) show excellent biocompatibility and unique viscoelastic characteristics. Here, we developed a novel HA membrane (HAM) by chemical crosslinking using 1,4-butanediol diglycidyl ether for the effective treatment of OSDs. The main purpose of HAMs is to provide sustained release of HA to modulate the wound healing response in OSDs. The safety and efficacy of HAMs were investigated using primary cultured human corneal epithelial cells and various OSD rabbit models. In the dry state, the HAM is firm, transparent, and easy to manipulate. When hydrated, it swells rapidly with high water retention and over 90% transmission of visible light. Human corneal epithelial cells and rabbit eyes showed no toxic response to HAM. Addition of HAMs to the culture medium enhanced human corneal epithelial cell viability and expression of cell proliferation markers. Investigation of HAM wound healing efficacy using mechanical or chemical corneal trauma and conjunctival surgery in rabbits revealed that application of HAMs to the ocular surface enhanced healing of corneal epithelium and reduced corneal limbal vascularization, opacity and conjunctival fibrosis. The therapeutic potential of HAMs in various OSDs was successfully demonstrated.

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Section: Characterization Of Ham Water Absorption Capacity and Opticmentioning

confidence: 99%

Development of a novel hyaluronic acid membrane for the treatment of ocular surface diseases

Kim

Jung

Pak

et al. 2021

Sci Rep

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“…For instance, HA exhibits suboptimal efficacy due to its inability to form physical (Type 2) hydrogels over a wide range of pH conditions. Additionally, it has a short half-life of about 12 h as it undergoes rapid degradation by the hyaluronase enzymes present in body tissues [5,6]. Therefore, cross-linked (Type 1) HA hydrogel films can overcome the above limitations and provide a robust cosmetic platform.…”

Section: Materials Chemicals and Reagentsmentioning

confidence: 99%

“…Key analytical assays for the characterisation of cross-linked hydrogels include swelling ratio and mesh size. In addition, cross-linked polymers are commonly analysed by Fourier Transform Infrared Spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR) [1,5]. FTIR and NMR are useful for identifying chemical changes, structural arrangements, and possible cross-linking mechanisms in hydrogels by comparing the chemical structure of the gel with those of its constituents [1].…”

Section: Preparation Of Hyaluronic Acid Hydrogelsmentioning

confidence: 99%

“…The x value was estimated to be 0.473 based on several assumptions [12,13]. Qv was calculated as Q m , which is the degree of mass swelling, according to Equation (5).…”

Section: Comparisons Of Average Mw Between Crosslinks Crosslinking Dmentioning

confidence: 99%

“…It contains repeated units of a disaccharide of β-1,4-D-glucuronic acid and β-1,3-N-acetyl-D-glucosamine ( Figure 1a). It occurs naturally in vivo as a polyanion in vitreous body fluids of the vitreous humour of the eye, synovial fluid of articular joints, and connective tissue [2,4,5].…”

Section: Introductionmentioning

confidence: 99%

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Studies on Novel Methods for Formulating Novel Cross-Linked Hydrogel Films of Hyaluronic Acid

2019

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Hyaluronic acid (HA) is a natural polysaccharide with promising applications in modern cosmetic and nutricosmetic products due to its high-water affinity, which is essential for skin hydration, as well as its biocompatibility, biodegradability, non-toxicity, and non-immunogenic nature. In this study, we investigated and optimized the method of crosslinking for formulating novel HA hydrogel films. We used Pentaerythritol Tetra-acrylate (PT) as the cross-linking agent over a range of pH values and used different cross-linking methods (Ultraviolet (UV) radiation, microwaving, and oven heating). The efficacy of the cross-linking reaction was evaluated using swelling studies and Fourier transform infrared (FTIR) spectroscopy for the characterization of the xerogel HA-PT film formulations. We found that HA-PT cross-linked hydrogels are produced under alkaline conditions (pH 11) but not under neutral or acidic conditions. Cross-linked HA-PT xerogel films using UV-irradiation showed excessive swelling indicative of inadequate cross-linking. The oven and microwaving methods produced HA-PT films with high cross-linking density. FTIR data suggest formation of ester bond between the carbonyl of the HA and hydroxyl group of the PT acrylate group. Overall, the oven method was considered better and easier than UV-radiation/microwave methods because it is safer, user-friendly and eco-friendly, and can process larger batches.

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Dual Network Electrode Binder toward Practical Lithium–Sulfur Battery Applications

Sun

Gao

et al. 2023

ACS Energy Lett.

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Lithium–sulfur (Li–S) batteries are widely regarded as one of the most promising next-generation energy storage devices due to their high energy density. Nevertheless, fast capacity fade caused by the tremendous volume changes of S-based cathodes and polysulfide shuttling during cycling remains to be effectively tackled prior to practical applications. Inspired by skeletal muscle, here, we develop a responsive network/confinement network blend (RCB) binder to remove the above bottlenecks. The as-developed binder is prepared through simply blending an aqueous hyaluronic acid (HA) solution and an oily solution with a tetrazole group-based copolymer of polyacrylonitrile and poly(ethylene glycol) bisazide. Hydrophobic tetrazole group-containing polymer (denoted as PCP) condenses in the mixture solution forming submicrometer-sized irregular spherical domains as the confinement network similar to myofilament, while water-soluble HA constructs a responsive network mimicking the fascia of skeletal muscle; meanwhile, the formed responsive network can coordinate the force among the confinement network by linking with PCP via ionic bonding. The RCB binder exhibits superior mechanical matching and adhesive properties and thus can effectively accommodate the huge volume change of S-based electrodes. Additionally, abundant polar groups such as carboxylic, amide, cyano, and tetrazole groups allow the RCB binder to effectively capture lithium polysulfide through strong anchoring effect. As a result, Li–S batteries assembled with a limited RCB binder dosage (5 wt % of the whole electrode film weight) exhibit remarkable improvement in both cycling and rate performance, even under high S loadings. Such skeletal muscle-inspired binder design helps boost the advance of S-based cathode binders toward practical battery applications.

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Characterization of Linear and Chemically Cross-linked Hyaluronic acid using Various Analytical Techniques Including FTIR, ESI-MS, H1 NMR, and SEM

Cited by 7 publications

References 0 publications

Development of a novel hyaluronic acid membrane for the treatment of ocular surface diseases

Development of a novel hyaluronic acid membrane for the treatment of ocular surface diseases

Studies on Novel Methods for Formulating Novel Cross-Linked Hydrogel Films of Hyaluronic Acid

Dual Network Electrode Binder toward Practical Lithium–Sulfur Battery Applications

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