Characterization of lipid productivity and fatty acid profile of three fast-growing microalgae isolated from Bengkulu for possible use in health application

Praharyawan, Swastika; Rahman, Delicia Yunita; Susilaningsih, Dwi

doi:10.11594/jtls.06.02.03

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…Kultur sianobakteria ditumbuhkan di dalam botol berukuran 600 mL dengan 500 mL media selama 7 hari (Praharyawan, Rahman, & Susilaningsih, 2016). Kultur diaerasi secara berkesinambungan dengan menggunakan aerator dan diberi penyinaran di bawah lampu neon 18 watt dengan intensitas cahaya 2500 lux pada suhu kamar.…”

Section: Sianobakteria Dan Kondisi Kultivasiunclassified

Peningkatan Kemurnian dan Toksisitas Ekstrak Pigmen C-Fikosianin dari Sianobakteria Laut Jaaginema sp. BTM-11 dengan menggunakan Kitosan dan Arang Aktif

Praharyawan

Setyaningsih

Susilaningsih

et al. 2019

JPBKP

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AbstrakC-fikosianin adalah senyawa pigmen-aksesori fotosintetik berwarna biru yang terkandung dalam sianobakteria. Jaaginema sp. BTM-11 merupakan sianobakteria laut yang memiliki potensi sebagai penghasil pigmen C-fikosianin karena kandungannya yang tinggi. Nilai ekonomis pigmen C-fikosianin sangat ditentukan oleh rasio kemurnian yang dimilikinya. Penelitian ini bertujuan untuk meningkatkan kemurnian pigmen C-fikosianin dari ekstrak sianobakteria laut Jaaginema sp. BTM-11 dengan menggunakan kitosan dan arang aktif. Variabel independen pada penelitian ini adalah konsentrasi kitosan (0,075-3,750 g/L) dan konsentrasi arang aktif (2,5-10 g/L). Toksisitas C-fikosianin diuji dengan menggunakan metode Brine Shrimp Lethality Test (BSLT) untuk mendapatkan nilai Konsentrasi Letal-50 (LC50). Penggunaan kitosan konsentrasi 0,3 g/L yang dilanjutkan dengan penggunaan arang aktif konsentrasi 5,0 g/L berhasil meningkatkan rasio kemurnian pigmen C-fikosianin sebesar 57,5 dan 167,5%, secara berturut-turut. Kapasitas pengikatan kitosan dan kapasitas penjerapan arang aktif secara signifikan berhasil meningkatkan kemurnian C-fikosianin dari awalnya 0,900±0,067 menjadi 2,408±0,171. Peningkatan rasio kemurnian C-fikosianin juga diikuti dengan peningkatan toksisitas fraksi C-fikosianin sianobakteria laut Jaaginema sp. BTM-11. Nilai LC50 ekstrak Jaaginema sp. BTM-11 sebelum pemurnian yang sebesar 15,75 ppm meningkat menjadi 12,06 ppm setelah dimurnikan dengan kitosan dan arang aktif. Enhancement of Purity and Toxicity of C-Phycocyanin Pigment Extracted from Marine Cyanobacteria Jaaginema sp. BTM-11 Using Chitosan and Activated CarbonAbstractC-phycocyanin is a blue colored accessory photosynthetic pigment found in cyanobacteria. Jaaginema sp. BTM-11 is marine cyanobacteria which is potential to be used as C-phycocyanin producer due to its high contents. The economical value of the blue pigment, C-phycocyanin, is determined by its purity. This research aimed to enhancing the purity of C-phycocyanin extracted from marine cyanobacteria of Jaaginema sp. BTM-11 using chitosan and activated carbon. The independent variables of this research were chitosan concentration (0.075-3.750 g/L) and activated carbon concentration (2.50-10.0 g/L). The toxicity test of C-phycocyanin was carried out using Brine Shrimp Lethality Test (BSLT) method to obtain the Lethal Concentration-50 (LC50) value. The application of 0.3 g/L chitosan continued by of 5.0 g/L activated carbon was successfully increased the purity of C-phycocyanin by 57.5 and 167.5%, respectively. Binding capacity of chitosan and adsorption capacity of activated carbon succeed to significantly increase the purity ratio of C-phycocyanin from 0.900±0.067 to 2.408±0.171. The enhancement of purity ratio of C-phycocyanin fraction extracted from Jaaginema sp. BTM-11 was also followed by the enhancement of its toxicity. LC50 of C-phycocyanin extract before purification (15.75 ppm) was lower compared to the C-phycocyanin fraction (12.06 ppm) after being treated with chitosan and activated carbon.

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Section: Sianobakteria Dan Kondisi Kultivasiunclassified

Peningkatan Kemurnian dan Toksisitas Ekstrak Pigmen C-Fikosianin dari Sianobakteria Laut Jaaginema sp. BTM-11 dengan menggunakan Kitosan dan Arang Aktif

Praharyawan

Setyaningsih

Susilaningsih

et al. 2019

JPBKP

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“…Important polysaccharides have also shown health-promoting effects for medical uses, such as reducing blood lipids (Ru et al, 2020). The alternative food, feed, or even fuels become more promising by utilizing microalgae that contain large amounts of lipid (Praharyawan et al, 2016;Zhu et al, 2016). Some microalgae species such as Botryococcus braunii can accumulate lipid > 75% of dry cell weight (Yeesang & Cheirsilp, 2014).…”

Section: Introductionmentioning

confidence: 99%

CO2 Sequestration Using Sodium Hydroxide and Its Utilization for Chlorella sorokiniana Biomass Production

Sadewo

Hidhayati²,

Ambarsari³

et al. 2022

J Bio Bio Edu

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Chlorella is widely used for its fast growth rate and easy cultivation with 14–30% lipid content and 36–59% dry weight. Thus, sodium hydroxide is used to increase carbon consumption, biomass, and metabolites productions in microalgae. This study was conducted to observe the effect of sodium hydroxide addition on biomass and metabolites production in photoautotrophic cultivated Chlorella sorokiniana. Microalgae C.sorokiniana (LIPI12-Al016) was obtained from the culture collection of Microalgae and Bioprocess Engineering Research Group laboratory, National Research and Innovation Agency. Then, the microalgae were cultivated in media with various concentrations of sodium hydroxide. Biomass production was measured by gravimetry, and carbon consumption was measured by acid-alkalimetry. Sodium hydroxide 60 mM gave the best growth, maximizing average carbon consumption to 691.8 mg.L-1 and biomass production to 598.3 mg.L-1. The utilization of NaOH in the medium did not increase the metabolites content, except for protein. Carbohydrate was the dominant metabolite among the others. Fatty acids profile mainly composed of C16 and C18 fatty acids, which are favorable for biodiesel production. These results gave an overview of the potency of microalgae C. sorokiniana as a CO2 mitigation agent and alternative sources of energy and nutrition.

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Production of α-linolenic Acid by an Oleaginous Green Algae Acutodesmus obliquus Isolated from Malaysia

Othman¹,

Jamaluddin²,

Ibrahim³

et al. 2019

J Pure Appl Microbiol

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The main purpose of this study is to provide information on the biochemical composition of the total lipid extract of three different native freshwater microalgae namely Acutodesmus obliquus CN01, Chlorella sp. Carolina-15-2069, and Chlorella vulgaris NIES-1269 at different growth phases, cultured under standardized conditions that will be useful for commercialization purposes due to the content of α-linolenic acid (ALA), a type of omega-3 fatty acids. We monitored growth, biomass accumulation, chlorophyll content, lipid content and fatty acids composition in all three freshwater microalgae. In addition, this study seeks to throw some light on the lipid content of a newly isolated freshwater green alga Acutodesmus obliquus CN01 which have shown to give significant quantities of ALA, an added nutritional value to this microalga oil. Acutodesmus obliquus CN01 exhibited the fastest growth rate among the three microalgae used. Biomass was shown to be enhanced in the nitrogen enrichment medium. The outcomes from this study indicated that newly isolated Acutodesmus obliquus CN01 from Malaysia cultured under normal condition showed fast growth rate with high lipid content and also high ALA content. Therefore, from this study, Acutodesmus obliquus CN01 was identified as species of choice to be further explored for its potential to produce omega-3 fatty acids.

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Characterization of lipid productivity and fatty acid profile of three fast-growing microalgae isolated from Bengkulu for possible use in health application

Cited by 3 publications

References 33 publications

Peningkatan Kemurnian dan Toksisitas Ekstrak Pigmen C-Fikosianin dari Sianobakteria Laut Jaaginema sp. BTM-11 dengan menggunakan Kitosan dan Arang Aktif

Peningkatan Kemurnian dan Toksisitas Ekstrak Pigmen C-Fikosianin dari Sianobakteria Laut Jaaginema sp. BTM-11 dengan menggunakan Kitosan dan Arang Aktif

CO2 Sequestration Using Sodium Hydroxide and Its Utilization for Chlorella sorokiniana Biomass Production

Production of α-linolenic Acid by an Oleaginous Green Algae Acutodesmus obliquus Isolated from Malaysia

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