Characterization of liposomes and silica nanoparticles using resistive pulse method

Rudzevich, Yauheni; Lin, Youxi; Wearne, Adam; Ordóñez, Antonio; Lupan, Oleg; Chow, Lee

doi:10.1016/j.colsurfa.2014.01.080

Cited by 12 publications

(7 citation statements)

References 26 publications

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“…Values of the coefficient of determination R 2 between labeled size and measured size at Q sh D 3.0 L/min and Q sh D 6.0 L/min were 0.99998 and 0.99990, respectively. Sometimes, particle suspensions labeled as size standards show significant differences between labeled and measured sizes and have wider size distributions (Rudzevich et al 2014), in contrast to the results from this study. Figure 1b shows relative size differences (measured mean size/labeled size) as a function of labeled size.…”

Section: Particle Sizecontrasting

confidence: 99%

“…SiO 2 nanoparticles have also been developed for a wide range of applications in the life sciences (Barahona et al 2015). Commercial silica particles are often polydisperse, with wider size distributions than those of PSL spheres with comparable mean size (Yook et al 2008;Wang et al 2008;Rudzevich et al 2014). To obtain size distributions that are sufficiently narrow for SSIS calibration, the polydispersions can be classified by a differential mobility analyzer (DMA; Liu and Pui 1974;Knutson and Whitby 1975) that is calibrated with NIST traceability (Mulholland et al 1996;Yook et al 2008;Wang et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of nanosized silica size standards

Kimoto

Dick

Hunt

et al. 2017

Aerosol Science and Technology

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Nanosized silica size standards produced with a sol-gel synthesis process were evaluated for particle size, effective density, and refractive index in this study. Particle size and effective density measurements were conducted following protocol from the National Institute of Advanced Industrial Science and Technology (AIST) in Japan. Particle sizes were measured via electrical mobility analysis using a differential mobility analyzer (DMA) at sheath flow rates (Q sh ) of 3.0 and 6.0 L/min and a constant aerosol flow rate (Q a ) of 0.3 L/min. The measured mean and mode diameters agreed well with the labeled sizes in the size range 40-200 nm, with differences ranging from 0.03% to 0.8%, well within the labeled expanded uncertainties (95% confidence intervals) of 1.8%-2.2%. The coefficient of variation (CV) of the size distribution was 0.012-0.027 for 40-200 nm. Particle sizes measured for 20 nm and 30 nm standards showed size differences with respect to the certified sizes of 1.7% and 8.3% at Q sh D 6.0 L/min, but the size distributions were narrow, with CV D 0.047-0.064. The average effective density for the range 40-200 nm measured with an aerosol particle mass analyzer (APM) was 1.9 g/cm 3 . The real component of the refractive index measured with an optical particle counter (OPC) was 1.41 at a wavelength of 633 nm. All properties (size, effective density, and refractive index) were stable and could be measured with good repeatability. From these evaluations, it was found that the nanosized silica size standards have good characteristics for use as size standards and constitute a feasible alternative to PSL particles.

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Section: Particle Sizecontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of nanosized silica size standards

Kimoto

Dick

Hunt

et al. 2017

Aerosol Science and Technology

View full text Add to dashboard Cite

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“…Rudzevich et al [72] counted 100 nm diameter synthetic liposomes and evaluated their size and velocity distributions by resistive-pulse measurements with a 160 nm diameter borosilicate pipette ( figure 6a,b). The current pulses were a few milliseconds long and quite similar to those in Chen et al [73] investigated the translocation dynamics of small unilamellar phospholipid vesicles (SUVs) through significantly smaller pipettes with the orifice diameter ranging from approximately 14 to 72 nm.…”

Section: (B) Liposomes/vesicles/virusesmentioning

confidence: 99%

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Wang

Mirkin

2017

Proc. R. Soc. A.

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Along with more prevalent solid-state nanopores, glass or quartz nanopipettes have found applications in resistive-pulse and rectification sensing. Their advantages include the ease of fabrication, small physical size and needle-like geometry, rendering them useful for local measurements in small spaces and delivery of nanoparticles/biomolecules. Carbon nanopipettes fabricated by depositing a thin carbon layer on the inner wall of a quartz pipette provide additional means for detecting electroactive species and fine-tuning the current rectification properties. In this paper, we discuss the fundamentals of resistivepulse sensing with nanopipettes and our recent studies of current rectification in carbon pipettes.

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“…Resistive-pulse sensing relies on measurements of the ion current flowing through a biological or solid-state nanopore or a nanopipette. − A nanoparticle (NP), a vesicle, − or a large molecule entering the nanopore orifice affects its conductance, causing a transient decrease in the ion current (resistive pulse). Nanopore-based techniques have been widely used to sequence DNA and detect singe molecules, , NPs, , viruses, and nanoparticle-bound species. , Although nanopore-based sensors are powerful tools for detecting and counting single entities, their applications to qualitative and quantitative analysis are more challenging.…”

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confidence: 99%

Electrochemical Resistive-Pulse Sensing

et al. 2019

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Resistive-pulse sensing with biological or solid-state nanopores and nanopipettes has been widely employed in detecting single molecules and nanoparticles. The analytical signal in such experiments is the change in ionic current caused by the molecule/particle translocation through the pipet orifice. This paper describes a new version of the resistive-pulse technique based on the use of carbon nanopipettes (CNP). The measured current is produced by electrochemical oxidation/reduction of redox molecules at the carbon surface and responds to the particle translocation. In addition to counting single entities, this technique enables qualitative and quantitative analysis of the electroactive material they contain. Using liposomes as a model system, we demonstrate the capacity of CNPs for (1) conventional resistive-pulse sensing of single liposomes, (2) electrochemical resistive-pulse sensing, and (3) electrochemical identification and quantitation of redox species (e.g., ferrocyanide, dopamine, and nitrite) contained in a single liposome. The small physical size of a CNP suggests the possibility of single-entity measurements in biological systems.

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Characterization of liposomes and silica nanoparticles using resistive pulse method

Cited by 12 publications

References 26 publications

Characterization of nanosized silica size standards

Characterization of nanosized silica size standards

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Electrochemical Resistive-Pulse Sensing

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