Characterization of Listeria monocytogenes isolates by Southern blot hybridization

Wesley, Irene V.; Wesley, Ronald D.; Heisick, Judy E.; Harrell, Fannie; Wagner, Dean E.

doi:10.1016/0378-1135(90)90182-u

Cited by 16 publications

(8 citation statements)

References 13 publications

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“…Apart from advanced and useful applications, such as fluorescence in situ hybridization (FISH) (Amann and others , ) and microarray technology, which will be described later in this review, several nucleic‐acid hybridization‐based assays, including dot‐blot (Kafatos and others ), Southern‐blot (Southern ) and Northern‐blot (Alwine and others ), colony hybridization (Grunstein and Hogness ), and colorimetric DNA hybridization (Leary and others ) have been developed to detect milk‐ and dairyborne pathogens (Hill and others ; Flowers and others ; King and others ; Curiale and Klatt ; Samadpour and others ; Wesley and others ; Kneifel and others ; Bottari and others ; Herman and others ; Vernozy‐Rozand and others ; Lamoureux and others ; Ng and others ; O'Connor and others ; Villard and others ; Stender and others ; Gómez and others ; Wang and others ; Gunasekera and others ; Schmid and others ; Lira and others ; Oliveira and others ; Stevens and Jaykus ; Amagliani and others ; Fuchizawa and others ; Pradel and others ; Baylis ; Almeida and others , , b; Mozola and others ; Zweifel and others ; Madic and others ). However, the lack of sensitivity of these assays limits their use to culture confirmation rather than being suitable for the direct monitoring of pathogens in milk and dairy products.…”

Section: Current Trend and Future Perspectives In Milk‐ And Dairybornmentioning

confidence: 99%

Culture‐Dependent and Culture‐Independent Nucleic‐Acid‐Based Methods Used in the Microbial Safety Assessment of Milk and Dairy Products

Fusco

Quero

2014

Comp Rev Food Sci Food Safe

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Despite great advances in the diagnostics and better awareness for food safety and security worldwide, significant numbers of foodborne outbreaks have been traced back to the consumption of milk and dairy products contaminated with pathogenic bacteria, such as Listeria monocytogenes, Staphylococcus aureus, Salmonella spp., Campylobacter spp., and pathogenic Escherichia coli. Several culture-dependent and culture-independent nucleic acid-based methods have been proposed to identify, detect, and type milk-and dairyborne pathogenic bacteria. In our review, we will provide an overview on why it is of utmost importance to ascertain the presence of pathogenic microorganisms in milk and milk products; thereafter, we will describe the most commonly used culture-dependent and culture-independent methods, as well as the most attractive ones with regard to their future exploitation, providing the reader with new insights into how and when they can be exploited to ensure the enumeration, and accurate detection at both species and strain level of the most important milk-and dairyborne pathogenic bacteria, even if in a viable but nonculturable state.

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Section: Current Trend and Future Perspectives In Milk‐ And Dairybornmentioning

confidence: 99%

Culture‐Dependent and Culture‐Independent Nucleic‐Acid‐Based Methods Used in the Microbial Safety Assessment of Milk and Dairy Products

Fusco

Quero

2014

Comp Rev Food Sci Food Safe

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“…DNA fingerprints can be further utilized by Southern blotting and hybridization with Listeria-specific probes. Gene probes have been constructed to detect various virulence factors, including hemolysins (5,40) and delayed hypersensitivity factors (23) in addition to other major secreted proteins (8,17). Many of these factors are present as single-copy genes in Listeria species, making it difficult to detect small numbers of organisms.…”

Section: Dmentioning

confidence: 99%

“…Numerous studies have demonstrated the utility of DNA fingerprinting for epidemiological analysis of phenotypically indistinguishable microbial pathogens (1,4,15,18,19,22,36,(38)(39)(40). Ribotyping, based on restriction fragment length polymorphisms in the chromosomal DNA containing rRNA genes, has also been used as a means of detecting interspecies and interstrain differences (13,14,24).…”

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confidence: 99%

Comparison of methods for discrimination between strains of Listeria monocytogenes from epidemiological surveys

Baloga

Harlander

1991

Appl Environ Microbiol

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Total cellular DNA from 28 strains of Listeria monocytogenes isolated from food implicated in food-borne illness and from patients with listeriosis was digested with the restriction endonucleases HindIII, HaeIII, and EcoRI. Following agarose gel electrophoresis, the fragments were subjected to Southern blot hybridization with a digoxigenin-labeled cDNA probe transcribed from Escherichia coli 16S and 23S rRNA. The patterns of bands from genomic (DNA fingerprints) and rDNA fingerprints (ribotypes) were used for classifying L. monocytogenes strains, and the resulting subtypes were compared with serotyping and multilocus enzyme electrophoresis classffication schemes. A total of 15 distinct and identical groups were obtained when genomic DNA was digested with either HindIII or HaeIII. The most discriminating enzyme for ribotyping of strains was EcoRI, which divided the 28 strains of L. monocytogenes into 6 ribotype groups. DNA fingerprinting and ribotyping differentiated L. monocytogenes from other Listeria spp., including L. ivanovii, L. welshimeri, and L. innocua as well as the lactic acid bacteria Lactococcus lactis subsp. lactis and subsp. cremoris. L. monocytogenes strains isolated from four independent food-borne illness incidents were analyzed by all typing methods. Patient and product isolates were not distinguishable by serotyping, ribotyping, or multilocus enzyme electrophoresis. DNA fingerprinting was the only method capable of differentiating these strains, or conversely, of proving relatedness of patient-product pairs of isolates. This method was a relatively simple, sensitive, reproducible, and highly discriminating method for epidemiological tracking of L. monocytogenes implicated in food-borne illness.

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“…(Zuerner and Bolin, 1990), Listeria sp. (Wesley et al, 1990), Mycobacterium paratuberculosis (McFadden et al, 1987(McFadden et al, , 1988, Mycobacterium tuberculosis (Eisenach et al, 1988;McFadden et al, 1988 ), Mycoplasma gallisepticum (Geary et al, 1988 ) and other Mycoplasma spp. (Razin et al, 1987 ).…”

Section: Detection Of Pathogens In Clinical Samplesmentioning

confidence: 99%

Applications of nucleic acid probes in veterinary infectious diseases

Paul

1990

Veterinary Microbiology

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Nucleic acid probe technology is increasingly being used in basic research in veterinary microbiology and in diagnosis of infectious diseases of veterinary importance. This review presents an overview of nucleic acid probe methodology and its applications in veterinary infectious diseases. The major applications of nucleic acid probes include detection of pathogens in clinical samples, especially those organisms which are fastidious and difficult to cultivate, differentiation of virulent from avirulent organisms and vaccine strains from wild type isolates, typing of microorganisms, mapping genes, screening libraries of cloned DNA for specific genes, detection of latently infected or carrier animals, study of mechanisms of pathogenesis, epidemiological studies and food safety.

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Characterization of Listeria monocytogenes isolates by Southern blot hybridization

Cited by 16 publications

References 13 publications

Culture‐Dependent and Culture‐Independent Nucleic‐Acid‐Based Methods Used in the Microbial Safety Assessment of Milk and Dairy Products

Culture‐Dependent and Culture‐Independent Nucleic‐Acid‐Based Methods Used in the Microbial Safety Assessment of Milk and Dairy Products

Comparison of methods for discrimination between strains of Listeria monocytogenes from epidemiological surveys

Applications of nucleic acid probes in veterinary infectious diseases

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