Characterization of Little millet (Panicum sumatrense) varieties using Morphological descriptors and SSR based DNA fingerprinting

Abstract

Little millet varieties are generally distinguished by morphological descriptors which are being used for seed certification and DUS characterization [1]. But in practical terms, these key differentiation descriptors between varieties of little millet are very fewer and hence difficult to differentiate germplasm accessions. Germplasm registration in NBPGR needs DNA fingerprint to show the uniqueness of germplasm in comparison to existing varieties. DNA fingerprinting is a better option to identify unique markers to differentiate the varieties. Available genomic resources are scarce since little millet is still considered to be an orphan crop. Therefore markers from other cereal genomes such as maize, pearl millet and barnyard millet that are been utilized for DNA fingerprinting purpose with a clue of cereal synteny relationship. Twenty-one morphological descriptors studies revealed that the variety ATL 1 is different from the other varieties for more than 16 morphological characters studied. DNA fingerprinting is attempted in five genotypes of little millets such as BL6, ATL 1, TNPsu 176, Co (Samai) 4, Paiyur 2 using cereal SSR markers. Among the 25 maize SSR markers used two markers viz., phi213984 and phi295450 scored polymorphism by the amplicon size of 310bp and 600bp respectively. From the 25 Pearl millet SSR markers used only one SSR marker found polymorphic at 305bp allele size for ATL 1 and Hence, SSR based DNA fingerprinting helped to differentiate ATL1, the newly released high yielding variety from other genotypes of little millets which can be used for varietal identification purpose.

Characterization of little millet (Panicum sumatrense Roth. ex. Roem. and Schultz) landraces and varieties for genetic diversity and association of traits

“…These qualitative traits could further be evaluated in other trials and could be used as morphological markers for the identification of true to type landraces from the hilly regions. Similar results for qualitative analysis and DUS characterization were also reported by (Natesan et al, 2020) Genotypes with arched inflorescence, compact panicle and round grains were included in the cluster I and cluster III (Table 9). Among these two clusters, cluster III contained genotypes with pigmentation at the leaf sheath while pigmentation was absent in the genotypes grouped under cluster I.…”

LMTdb: A comprehensive transcriptome database for climate-resilient, nutritionally rich little millet (Panicum sumatrense)

“…As a result of that, unlike rice, wheat, and other cereals, the crop has still not achieved breakthroughs in terms of gene discoveries, genetic improvement, and yield enhancement. Also, due to a lack of enough studies, ‘Omics’ information related to the crop has remained scarce ( Natesan et al., 2020 ).…”

Establishment of DNA Molecular Fingerprint of Caladium Core Collections

“…The current methods of fingerprint construction include polyacrylamide gel electrophoresis (PAGE), capillary electrophoresis with fluorescence detection, etc. [35][36][37]. With the rapid development of capillary electrophoresis with fluorescent SSR markers to construct DNA fingerprints, the obtained results can be converted into strings, barcodes, and quick response (QR) codes with the help of fingerprints [38,39].…”