2012
DOI: 10.5402/2012/287230
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Characterization of LlaKI, a New Metal Ion-Independent Restriction Endonuclease from Lactococcus lactis KLDS4

Abstract: Requirement of divalent cations for DNA cleavage is a general feature of type II restriction enzymes with the exception of few members of this group. A new type II restriction endonuclease has been partially purified from Lactococcus lactis KLDS4. The enzyme was denoted as LlaKI and showed to recognize and cleave the same site as FokI. The enzyme displayed a denatured molecular weight of 50 kDa and behaved as a dimer in solution as evidenced by the size exclusion chromatography. To investigate the role of diva… Show more

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Cited by 4 publications
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“…This enzyme has been investigated in detail with regard to protein maturation, secretion, catalytic mechanism, and biotechnological applications [2]. Further NSNs, especially metal-ion-independent enzymes, have only been rarely investigated to date [13,16,[18][19][20]23].…”
Section: Discussionmentioning
confidence: 99%
“…This enzyme has been investigated in detail with regard to protein maturation, secretion, catalytic mechanism, and biotechnological applications [2]. Further NSNs, especially metal-ion-independent enzymes, have only been rarely investigated to date [13,16,[18][19][20]23].…”
Section: Discussionmentioning
confidence: 99%