Characterization of Longidorus caespiticola (Nematoda: Longidoridae) from the Czech Republic

Abstract

153 SummaryLongidorus caespiticola was found for the first time from the rhizosphere of apple with two other Longidorus sp. at Žlunice, Czech Republic. Females and males were analyzed morphologically and morphometrically. Four regions (18S, ITS1, ITS2, D2-D3 expansion segments of 28S) of ribosomal DNA and one region (cox1) of mitochondrial DNA were sequenced and analyzed and compared with a population from the Slovak Republic. The partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene showed relatively high genetic variation compared with ribosomal DNA between the Czech and Slovakian populations.

First Reports, Morphological, and Molecular Characterization of Longidorus caespiticola and Longidorus poessneckensis (Nematoda: Longidoridae) from Ukraine

“…During recent nematode surveys in Ukraine, three needle nematode populations were detected, one resembling L. caespiticola Hooper, 1961 and two populations resembling L. poessneckensis Altherr, 1974. Both these species were previously reported from several other European countries Kornobis and Peneva, 2011;Kumari, 2014), but not from Ukraine. Therefore, the objective of the present study was to provide an accurate identification of Longidorus species detected in Ukraine by an integrative approach of morphological and molecular characterization by using the D2-D3 expansion segments of 28S rRNA.…”

“…Ukrainian specimens of L. caespiticola in comparison to Slovenian (Sirka and Urek, 2009) have shorter body length ($: 6.03 (5.22-7.39) vs 7.84 (7.07-8.64) mm; #: 5.95 (5.61-6.52) vs 7.24 (6.24-8.46) mm) and more slender body; lower a ratio $: 82.4 (74. Finally, it differs from Czech population (Kumari, 2014) in a ratio higher in female (82.4 (74.3-90.3) vs 70.1 (63.1-78.3)) but lower in male (80.6 (66.8-95.2) vs 87.0 (76.4-100.0) and more slender body at the level of midbody (diam. $: 73.1 (67-83) vs 92 (83-111); #: 68.4 (58-78) vs 73 (65-88) mm) and anus (diam.…”

“…The main differences between Ukrainian population of L. caespiticola and other populations of this species are slightly shorter odontostyle ($: 93.9 (89-97) vs 110 (109-114) mm (British) (Hooper, 1961); 102.5 (96.1-109.3) mm (Slovenian) (Sirka and Urek, 2009); 96 (90-104) mm (Czech) (Kumari, 2014); #: 95.5 (89-101) vs 105 (99-111) mm (British); 103.8 (94.5-108.7) mm (Slovenian); 98 (93-103) mm (Czech)); and lower number of supplements (9-13 vs 15-20 (British); 12-18 (Czech)).…”

Phylogenetic studies on three Helicotylenchus species based on 28S rDNA and mtCOI sequence data

“…The mitochondrial DNA region encoding for mtCOI gene has been widely explored for barcoding application purposes in many animal organisms (Rach et al, 2017). It was also used in Hoplolaimidae and other nematode groups (Kanzaki and Futai, 2002;Derycke et al, 2010;Riepsamen et al, 2011;Gutiérrez-Gutiérrez et al, 2012;Kumari and Subbotin, 2012;van den Berg et al, 2013;Holguin et al, 2015;van den Berg et al, 2017;Sanchez-Monge et al, 2017;Skwiercz et al, 2017). In this study, to enhance the process of delineation and species identification within genus Helicotylenchus, which was underrepresented in terms of available mtCOI sequences, a new set of mtCOI primers was developed and tested.…”

First report with morphometrics and molecular characterization of phtyonematodes associating mango trees in the tropics of Saudi Arabia

“…Nematodes were then mounted in a fresh formalin solution (2%) on microscopic glass slides for examination (Shurtleff and Averre III, 2000). Stylet-bearing nematodes were morphologically identified either to genera and/or species according to the standardized taxonomical keys (Goodey, 1963, Mai and Lyon, 1975, Siddiqi, 1977, Handoo and Palomares-Rius, 2014, Kumari, 2014).…”