Characterization of low temperature-induced plasma membrane lipidome remodeling combined with gene expression analysis reveals mechanisms that regulate membrane lipid desaturation in Carica papaya

Chen, Mingjie; Liu, Ruiming; Huang, Xiaohui; Du, Zhenghua; Heng, Shuangpin; Zeng, Wei

doi:10.1016/j.scienta.2020.109505

Cited by 9 publications

(6 citation statements)

References 37 publications

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“…Cold stress is a kind of abiotic stress that can considerably limit both plant growth and yield and can determine the geographic distribution of plants 1 . Therefore, freezing tolerance acts as an important quantitative trait and has received considerable amounts of attention for the past several decades 2 – 5 . In model plant species, researchers have identified complex signaling networks consisting of cold sensors, secondary messengers, transcription factors (TFs), phytohormones and functional proteins 6 – 9 .…”

Section: Introductionmentioning

confidence: 99%

The AaCBF4-AaBAM3.1 module enhances freezing tolerance of kiwifruit (Actinidia arguta)

Sun

et al. 2021

Hortic Res

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Beta-amylase (BAM) plays an important role in plant resistance to cold stress. However, the specific role of the BAM gene in freezing tolerance is poorly understood. In this study, we demonstrated that a cold-responsive gene module was involved in the freezing tolerance of kiwifruit. In this module, the expression of AaBAM3.1, which encodes a functional protein, was induced by cold stress. AaBAM3.1-overexpressing kiwifruit lines showed increased freezing tolerance, and the heterologous overexpression of AaBAM3.1 in Arabidopsis thaliana resulted in a similar phenotype. The results of promoter GUS activity and cis-element analyses predicted AaCBF4 to be an upstream transcription factor that could regulate AaBAM3.1 expression. Further investigation of protein-DNA interactions by using yeast one-hybrid, GUS coexpression, and dual luciferase reporter assays confirmed that AaCBF4 directly regulated AaBAM3.1 expression. In addition, the expression of both AaBAM3.1 and AaCBF4 in kiwifruit responded positively to cold stress. Hence, we conclude that the AaCBF-AaBAM module is involved in the positive regulation of the freezing tolerance of kiwifruit.

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Section: Introductionmentioning

confidence: 99%

The AaCBF4-AaBAM3.1 module enhances freezing tolerance of kiwifruit (Actinidia arguta)

Sun

et al. 2021

Hortic Res

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“…PG in extraplastidic membranes is nearly exclusively of eukaryotic structure with 18C fatty acids at the sn -2 position [ 35 , 36 ]. PG 34 (16/18) is the dominant PG in plasma membranes [ 36 , 37 ], while trace amounts of PG 36 (18/18) have been reported [ 35 ]. Fritz et al (2007) and Burgo et al (2011) found Δ 3-trans in sn -2 bound cis -unsaturated 18C fatty acids of PG [ 38 , 39 ], which raises the question whether FAD4 is responsible for introducing this trans -double bond.…”

Section: Resultsmentioning

confidence: 99%

Plastid Phosphatidylglycerol Homeostasis Is Required for Plant Growth and Metabolism in Arabidopsis thaliana

et al. 2023

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A unique feature of plastid phosphatidylglycerol (PG) is a trans-double bond specifically at the sn-2 position of 16C fatty acid (16:1t- PG), which is catalyzed by FATTY ACID DESATURASE 4 (FAD4). To offer additional insights about the in vivo roles of FAD4 and its product 16:1t-PG, FAD4 overexpression lines (OX-FAD4s) were generated in Arabidopsis thaliana Columbia ecotype. When grown under continuous light condition, the fad4-2 and OX-FAD4s plants exhibited higher growth rates compared to WT control. Total lipids were isolated from Col, fad4-2, and OX-FAD4_2 plants, and polar lipids quantified by lipidomic profiling. We found that disrupting FAD4 expression altered prokaryotic and eukaryotic PG content and composition. Prokaryotic and eukaryotic monogalactosyl diacylglycerol (MGDG) was up-regulated in OX-FAD4 plants but not in fad4-2 mutant. We propose that 16:1t-PG homeostasis in plastid envelope membranes may coordinate plant growth and stress response by restricting photoassimilate export from the chloroplast.

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“…PA synthesis and decomposition occur via multiple pathways and are therefore highly dynamic and complex [ 42 ]. PA promotes the negative curvature of the membrane bilayer and increases the permeability of membrane vesicles in vitro [ 43 ]. Furthermore, high PA levels can disrupt membrane stability, thereby causing the loss of membrane integrity and membrane protein function during injury, senescence, freezing, and drought [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Lipidomic and transcriptomic profiles of glycerophospholipid metabolism during Hemerocallis citrina Baroni flowering

Yang

Jiang

et al. 2023

BMC Plant Biol

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Background Hemerocallis citrina Baroni (daylily) is a horticultural ornamental plant and vegetable with various applications as a raw material in traditional Chinese medicine and as a flavouring agent. Daylily contains many functional substances and is rich in lecithin, which is mostly composed of glycerophospholipids. To study the comprehensive dynamic changes in glycerophospholipid during daylily flowering and the underlying signalling mechanisms, we performed comprehensive, time-resolved lipidomic and transcriptomic analyses of ‘Datong Huanghua 6’ daylily. Results Labelling with PKH67 fluorescent antibodies clearly and effectively helped visualise lipid changes in daylily, while relative conductivity and malonaldehyde content detection revealed that the early stages of flowering were controllable processes; however, differences became non-significant after 18 h, indicating cellular damage. In addition, phospholipase D (PLD) and lipoxygenase (LOX) activities increased throughout the flowering process, suggesting that lipid hydrolysis and oxidation had intensified. Lipidomics identified 558 lipids that changed during flowering, with the most different lipids found 12 h before and 12 h after flowering. Transcriptome analysis identified 13 key functional genes and enzymes in the glycerophospholipid metabolic pathway. The two-way orthogonal partial least squares analysis showed that diacylglycerol diphosphate phosphatase correlated strongly and positively with phosphatidic acid (PA)(22:0/18:2), PA(34:2), PA(34:4), and diacylglycerol(18:2/21:0) but negatively with phospholipase C. In addition, ethanolamine phosphotransferase gene and phospholipid-N-methyltransferase gene correlated positively with phosphatidylethanolamine (PE)(16:0/18:2), PE(16:0/18:3), PE(33:2), and lysophosphatidylcholine (16:0) but negatively with PE(34:1). Conclusions Overall, this study elucidated changes in the glycerophospholipid metabolism pathway during the daylily flowering process, as well as characteristic genes, thus providing a basis for future studies of glycerophospholipids and signal transduction in daylilies.

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Characterization of low temperature-induced plasma membrane lipidome remodeling combined with gene expression analysis reveals mechanisms that regulate membrane lipid desaturation in Carica papaya

Cited by 9 publications

References 37 publications

The AaCBF4-AaBAM3.1 module enhances freezing tolerance of kiwifruit (Actinidia arguta)

The AaCBF4-AaBAM3.1 module enhances freezing tolerance of kiwifruit (Actinidia arguta)

Plastid Phosphatidylglycerol Homeostasis Is Required for Plant Growth and Metabolism in Arabidopsis thaliana

Lipidomic and transcriptomic profiles of glycerophospholipid metabolism during Hemerocallis citrina Baroni flowering

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