Characterization of &lt;i&gt;Pyrenophora tritici-repentis, Parastagonospora nodorum &lt;/i&gt;and &lt;i&gt;Parastagonospora pseudonodorum &lt;/i&gt;populations based on the presence of effector genes in the Tambov Oblast territory

Kovalenko, N. M; Zeleneva, Yu. V; Sudnikova, V. P

doi:10.31857/s2500262723020114

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…В Тамбовской области в последнее время наибольшую экономическую значимость приобретают септориозные и пиренофорозные пятнистости. Распространение их заметно варьирует в зависимости от погодных условий и сортовых особенностей культуры (Zeleneva et al, 2022;Kovalenko et al, 2023).…”

Section: Introductionunclassified

Resistance of spring bread wheat cultivars and lines to Septoria leaf blotch, tan spot, and spot blotch pathogens

Zeleneva,

Sudnikovа,

Kovalenkо

et al. 2023

Trudy po prikladnoy botanike, genetike i selektsii

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Background. Leaf and stem diseases of cereal crops are among the most harmful in the agrocenoses of Tambov Province, significantly reducing grain yield and rapidly progressing in many regions of Russia. The aim of the study was to identify spring bread wheat lines and cultivars developed at the Middle-Russian Branch of the I.V. Michurin Federal Science Center that show resistance to leaf and stem diseases.Materials and methods. The target material was represented by 3 cultivars and 23 breeding lines of spring bread wheat. Disease resistance in seedlings was assessed in the laboratory, while for adult plants the process was carried out in the field in 2020–2022. The presence of the Tsn1 allele controlling susceptibility to the PtrToxA fungal toxin was detected using the Xfcp623 molecular marker.Results and conclusions. Accessions with high levels of resistance to Zymoseptoria tritici were identified: L-8252, L-82/60, L-8107, L-8078(23), L-43-9, L-43-1, L-4, Stb-8/15, Stb-7/15, and Stb-92. Accessions Stb-97, Stb-89, Stb-89(a), Stb-90, Stb-34, and Rl-6-22 were resistant to Parastagonospora nodorum; L-8134, L-8114, L-82/60, L-8107, Stb-10/15, Stb-9/15, Stb-8/15, Stb-7/15, Stb-97, Stb-89, Stb-89(a), Stb-90, Stb-34, and Rl-6-22 to P. pseudonodorum; L-8252, L-8107, L-43-9, L-43-1, L-4, Stb10/15, Stb-9/15, Stb-97, Stb-92, Stb-92(a), Stb-89, Stb-89(a), and Stb-34 to Pyrenophora tritici-repentis; and L-8114, L-82/60, L-43-9, L-43-1, L-4, Stb-90, Stb-34, and Rl-6-22 to Bipolaris sorokiniana. The cultivars and breeding lines showed group resistance to two, three or four pathogens. It was Lines L-8252, L-8134, L-82/60, L-8107, L-8078 (23), L-43-9, L-43-1, L-4, Stb-89, Stb-90, Stb-34, and L-33809-7-3 were observed to carry the recessive allele of the tsn1 gene.

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Section: Introductionunclassified

Resistance of spring bread wheat cultivars and lines to Septoria leaf blotch, tan spot, and spot blotch pathogens

Zeleneva,

Sudnikovа,

Kovalenkо

et al. 2023

Trudy po prikladnoy botanike, genetike i selektsii

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Molecular Identification, Effector genes and Virulence of Isolates of <i>Parastagonospora nodorum</i> from Altai Krai (Russia)

Zeleneva,

Gannibal,

Kazartsev

et al. 2023

Mikologiâ i fitopatologiâ

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Septoria blotches represent some of the most harmful wheat diseases that encompass all grain-producing countries worldwide. They are caused mainly by fungi of the genera Parastagonospora and Zymoseptoria. The aims of the research were to analyze the nucleotide sequences of two phylogenetically informative DNA loci, ITS and tub2, of Parastagonospora nodorum isolates from the Altai Krai, to detect the presence of Tox1, Tox3, ToxA effector genes and to study the virulence of the isolates. Microscopic analysis of fragments of affected plant tissue was used for primary identification of the Septoria blotch. The causative agent from the studied plant samples was identified as P. nodorum. Fungal colonies cultivated on potato-glucose agar displayed a well-developed light brown velvety mycelium with dark periphery (mixed type) and numerous pycnidia exhibiting high sporulation capacity. Microbiological diagnostics were complemented by molecular genetic studies. Sequencing of ITS and tub2 loci isolates revealed complete genetic identity of all eight studied monoconidial isolates obtained from different samples. PCR-based detection of tree Tox-genes demonstrated that the only Tox3 was present in the geno-types of 80 tested P. nodorum monoconidial isolates. The ToxA and Tox1 genes were not found in the studied isolates. The virulence of P. nodorum isolates was evaluated in laboratory conditions using an isolated wheat leaves assay. Mixture of four isolates from spring wheat and one isolate from winter wheat, were characterized as virulent. When spring and winter wheat cultivars were infected with two isolates obtained from oats, pathogenic but avirulent properties were detected. The isolate from triticale was avirulent to winter wheat cultivars and virulent to spring wheat cultivars.

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Characterization of <i>Pyrenophora tritici-repentis, Parastagonospora nodorum </i>and <i>Parastagonospora pseudonodorum </i>populations based on the presence of effector genes in the Tambov Oblast territory

Cited by 2 publications

References 22 publications

Resistance of spring bread wheat cultivars and lines to Septoria leaf blotch, tan spot, and spot blotch pathogens

Resistance of spring bread wheat cultivars and lines to Septoria leaf blotch, tan spot, and spot blotch pathogens

Molecular Identification, Effector genes and Virulence of Isolates of <i>Parastagonospora nodorum</i> from Altai Krai (Russia)

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